子控制

New strategies for breeding seedless plants have been proposed by using these genes. These include expression of some cytotoxin genes or genes for production of hormones in plants under the control of seed_coat_specific promoter or ovary_specific promoter and "terminator technology" etc. It's clear that these transgenic methods will be introduced into a wider range of fruit and vegetables in the future.

应用这些目的基因，已建立了转基因生产无籽果实的新策略，如在种皮或子房特异性启动子控制下的生长素基因或细胞毒素基因的表达及"终止子"技术的运用等，这将大规模地促进蔬菜和水果生产，提高果蔬产品的市场价值。

To prove that the cloned DNA fragment can express tryptopanase,a new plasmid pET28C-TnaA , in which the cloned DNA fragment was located downstream of T7 promoter on pET28c was constructed and transformed into host BL21(DE3),a BL21 lysogen of bacteriophage DE3 in which the only promoter known to direct transcription of the T7 RNA polymerase gene is the lacuvS promoter ,which is inducible by IPTG.

为了证明质粒上的基因能表达出有活性的色氨酸酶，将这个DNA片段克隆到PET28c质粒的BamHI和HindⅢ位点上，使该片段受T7 RNA聚合酶的启动子控制，然后转化噬菌体DE3的溶源菌BL21(DE3)。

In this paper, the fib-H promoter was choned by PCR method and sequenced. Sequence analysis showed that the cloned fib-H promoter harbored the typical motifs, which resulted in the gene expressing in the silk gland specifically.

为利用其时空调控机制，通过PCR扩增方法克隆fib-H启动子片段，并进行序列分析，进而构建由fib-H启动子控制报告基因DsRed的重组载体pSK-FH-DsRed-PolyA，通过家蚕BmN细胞和丝腺进行瞬时表达。

The research work involving the expression manner of doxA gene in S. lividans TK24 with plasmid pYG57 implied that the promoter PermE may control its expression constitutively, the time for maximum expression emerged from 48 to 60 h after inoculation and cultivation and the expression level was kept relatively stable, furthermore, the recombinant hydroxylase existed mostly in mycelium cell but little in broth.

而对Tm4（pYG57）菌株中doxA基因表达方式的研究表明， dd基因在 PermE启动子控制下可能是一种组成型控制表达，其表达量在接种后培养到 48－60 h左右最高并且维持相对稳定，并且表达的柔红霉素 Cl4羟化酶主要存在于菌丝体细胞内，很少分泌到细胞外。

The SDS-PAGE electrophoresis indicated that all engineered strains containing different expression plasmid could apparently express a 45 kD band specific for daunorubicin C-14 hydroxylase. The expression level of doxA gene was much higher when the doxA gene was under the control of promoter PermE. However, it was found that fd terminator had few effect on the expression of doxA gene.

SDS-PAGE蛋白电泳实验证明，上述五个菌株都能够表达大小约45 kD的柔红霉素C-14羟化酶蛋白，并发现以在PermE启动子控制下的doxA基因表达量相对较高，而终止子对＠中英文摘要 dd基因表达似乎没有影响。

Five plasmids for the expression of doxA gene, which were designated as pYG57, pYG502, pYG503, pYG505 and pYG506 were constructed so that the cloned doxA gene were under the control of either promoter of melanin gene or erythromycin resistant gene or their tandem promoters, and a fd terminator downstream.

构建了五个doxA基因链霉菌表达质粒pYG57、pYG502、pYG503、pYG505和pYG506，使得doxA基因在上游的Pmel启动子、红霉素抗性基因启动子或者两者的串联启动子，以及下游的fd终止子控制之下。

Different plant expression vectors had been respectively transferred into upland cotton cultivars Jinman12, Jinmian7, Xinluzao1 and Jihe321 via Agrobacterium tumfaciens transformation. These vectors carried cryIAc3 gene, which encodes Bacillus thuringiensis δ-endotoxin, under the control of chimeric OM promoter, CaMV 35S promoter, and cotton leaf curl virus RP promoter respectively; snowdrop lectin gene (Galanthus nivals agglutinin, gna) trans-regulated by rolC promoter controlled TGAla factor; and multivalent expression construct of both cryIAc3-cpti fusion gene and gna gene. A large number of transgenic plants and their progenies had been obtained.

为了提高外源基因的表达量，延缓害虫产生耐受性，本文通过将携带有分别在高效复合OM启动子，CaMV35S启动子及棉花曲叶病毒RP启动子控制下的苏云金杆菌杀虫毒蛋白基因cryIAc3；反式调节因子增强韧皮部表达的雪花莲外源凝集素(Galanthus nivals agglutinin，GNA)基因；以及同时含有cryIAc3、豇豆胰蛋白酶抑制剂基因和gna三价抗虫基因的植物表达载体，以根农杆菌介导法分别将这些表达载体导入了陆地棉新陆早1号、晋棉7号、晋棉12号和冀合321等我国西北棉花主要栽培品种，经体细胞胚再生获得了大批转基因再生植株。

The results indicated that pac could be expressed (transcribed, transported and maturated) efficiently under the control of 〓 promoter.

可见，在〓启动子控制表达系统中，采用它自己编码的转录酶转录，不再受大肠杆菌代谢调控网络的控制。

The results showed that OsAgpS1a transcripts and the reporter gene GUS driven by the OsAgpS1a promoter had a similar expression pattern that abundantly expressed in endosperms but very low in leaves, whereas OsAgpS1b transcripts and the reporter gene GUS driven by the OsAgpS1b promoter own other similar expression pattern that expressed at a low level in leaves, roots and early development endosperms of rice.

结果表明，两个启动子与其下游转录本的表达模式完全一致，即OsAgpS1a转录本和OsAgpS1a上游启动子控制的GUS基因主要在胚乳中高水平表达，在叶片中有很低水平的表达；而OsAgpS1b转录本和OsAgpS1b上游启动子控制的GUS基因主要在叶片和胚乳发育早期低水平表达。

These results suggested that the function of each function domain in HIV 1 LTR exhibited distinct characteristics on gag gene expression in vaccinia virus:(1) The function of intact LTR on gag gene expression varied with the upstream poxvirus promoter;(2) NR sequence neither decreased nor increased the Gag protein exprssion level;(3) Enhancer sequence might not be recognized by recombinant vaccinia virus expression system;(4) TAR sequence enhanced Gag protein expression effectively;(5) U5 region and its downstream non translated sequence had little effect on

免疫印迹和免疫酶试验检测均表明，6株重组病毒的Gag蛋白表达量因LTR不同而有明显差异，表明HIV1的LTR及其下游基因置于痘病毒启动子控制下，在痘苗病毒中表达时有下述特点：（1）不同的痘苗病毒启动子与全长LTR相互作用，对gag基因表达有显著不同的调控效果；（2）NR序列对Gag蛋白表达没有明显影响；（3）EN序列不能被重组痘苗病毒表达系统识别；（4）TAR序列可提高Gag蛋白的表达量；（5）U5区及下游非翻译序列不影响Gag蛋白的表达。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了