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子孢子体

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Alternation of generations can be divided into two basic types, one for the same shape alternation of generations, the sporophyte and the gametophyte in the morphology similar size, only cytology and physiology are different, such as Ulva, etc.; another type of shape alternation of generations, that is, in the sporophyte and gametophyte morphology and size are also significant differences.

世代交替又可分为两种基本类型,一为同形世代交替,即孢子体和配子体在形态大小上类似,仅在细胞学上和生理上有所不同,如石莼等;另一类型为异形世代交替,即孢子体和配子体在形态结构和大小上也有明显差异。

Following 72 h co-culture, desquamate, sporozoites, trophozoites, meronts, microgametocytes, macrogametocytes, zygote, thin-wall oocyst, and thick-wall oocyst appeared orderly. Between the 60th and 72th hour, many oocysts emerged. Inoculated by the C. parvum -infected cell culture supernatant at the 48th hour, the immunosuppressed mice became infected.

在感染后72 h内,隐孢子虫出现连续发育阶段,包括脱囊、子孢子、裂殖子、裂殖体、滋养体、配子体、合子、薄壁卵囊和厚壁卵囊,在60~72 h内形成卵囊;用感染48 h的细胞培养上清接种于免疫抑制小鼠, 10 d后有隐孢子虫卵囊排出。

The author first reported the social phenomenon and synchronizing development incryptosporidia,the dynamic process of the sporozoites or merozoites aparted from theirparent substances and developed into trophozoites,the culture of C.baileyi in chickenembryo,and the excysting process without both the trypsin and the sodium taurocholatein 37℃ water.The high levels of excystation(60%)oceured at 37℃ water showedthat temprature was very important in cryptosporidia excysting process.

在国内首次观察和描述了薄壁型隐孢子虫卵囊;首次报道隐孢子虫具有群体寄生现象和同步发育特征;首次描述了隐孢子虫的裂殖子或子孢子离开母体和发育为滋养体的动态变化过程;首次应用粪便中纯化的C.baileyi卵囊感染鸡胚成功,并用37℃水脱囊,其脱囊率高达60%,表明温度对隐孢子虫的脱囊具有重要作用,无需胰酶和牛胆酸钠的参与。

The spore germination and gametophyte development of 21 ferns are studied. It indicates that :① the development period of prothallium and young sporophyte of the same species is different due to seeding time of spores;② the optimal temperatures of spores germination and gametophyte development of 21 ferns are about 15 -24 ℃;③ the rate of spore germination of rare ferns is much lower than the ferns which occur large populations in nature;④ the treatment with GA 3 can accelerate the germination of the spores;⑤ the prothallium changes from large to small, green to yellow when young sporophyte comes out. The prothalliums of all 21 ferns die away after the appear of the 3rd leaf of sporophytes;⑥ shapes of the 1st and 2nd leaves of the young sporophyte are different from those emerged later;⑦ spores cultured in dark can not germinate;⑧ a prothallium can develop only one young sporophyte though it has many archegoniums, and the spore propagation with leaf mould substrate is an economical method.

摘 要:以腐叶土为培养基质,对 21 种蕨类植物进行了孢子萌发和原叶体发育的研究,结果表明:①不同时期播种的同种蕨类的孢子,发育出原叶体和幼孢子体所历经的时间长短不同;②孢子萌发和配子体生长发育的适宜温度约为 15 ~ 24 ℃;③稀有蕨类的孢子萌发率低,而在野外能形成较大种群的蕨类的孢子萌发率高;④用 GA 3 处理孢子可以促进萌发;⑤当原叶体上长出幼孢子体时,原叶体由大变小,由绿变黄, 21 种蕨类的原叶体都在幼孢子体上长出第 3 片叶时消失;⑥幼孢子体上长出的第 1 、 2 片叶在形态上与以后长出的叶不同;⑦孢子萌发需要光;⑧ 1 片原叶体尽管有多个颈卵器,但仅发育出 1 株幼孢子体;⑨利用腐叶土进行蕨类孢子繁殖是一种经济实用的繁殖方法。

In addition, the results of male gametophytic selection would be effect the phenotype of offspring in sporophyte due to about two-thirds of gene expression proceed during the growth stage of gametophyte and sporophyte.

此外,由于植物基因组中约有2/3的基因表达交错发生在配子体阶段和孢子体阶段,因此雄配子体选择的结果会影响到下一代孢子体的表现型。

The development of a gametophyte directly from a sporophyte without the occurrence of meiosis or spore formation.

无孢子形成,无孢子生殖,无孢子状态没有经过减数分裂或孢子形成而直接由孢子体进行的配子体发育

After 13 months culture, sporophytes began to form. Each gametophyte had several archegonia, and only one sporophyte was produced per gametophyte. The first sporophyte frond was simple and had pinnate venation.

抱子体在经13个月的培养后开始产生,每个配子体虽然有许多的藏卵器,但只产生一株孢子体,第一片孢子体的叶片为单叶羽状脉。

Trophozoite,macrogamout,microgamout, typeⅠmeront,typeⅡmeront and sporozoite in the development of C.andersoni were observed by TEM.The proliferation of C.andersoni was significantly inhibited by 1.25μg/ml NTZ and 0.31μg/ml GAs.Treated with 20μg/ml NTZ and 5μg/ml GAs respectively,the numbers of C.andersoni were only 0.29%and 0.66%compared with the untreated.

透射电镜在HCT-8细胞中观察到安氏隐孢子虫的滋养体、雌配子体、雄配子体、Ⅰ型裂殖体、Ⅱ型裂殖体和子孢子阶段。1.25μg/ml NTZ和0.31μg/ml GAs即可显著抑制HCT-8细胞中安氏隐孢子虫的增殖。20μg/ml NTZ和5μg/ml GAs作用后,虫体数量仅分别为未加药的0.29%和0.66%。

Epiphytic bacteria were isolated from sporophytic thallus collected from Qingdao and Dalian or gametophytic filaments stored in our lab of Laminaria japonica. Identification was done using both traditional physiological and biochemical way and molecular method. Phylogeny tree was established based on 16S rRNA gene sequence analyses, and some sequences of epiphytic strains from both sporophyte and gametophyte were different from all the sequences already exit and could be the new species.

采用生理生化和16S rRNA基因序列比对的方法对从两个海区采集的海带孢子体以及实验室中保存的海带配子体样品的外生菌分别进行组成分析,16S rRNA基因序列揭示了在海带孢子体和配子体的外生菌中都存在一些与现有数据库中序列的遗传关系较远的菌株。

Polysomes change in number as "few-many-few-many" during the development of the egg, early zygote stage, zygote at dormancy, zygote in prophase of mitosis and two-celled proembryo in sugar beet, which indicates that such changes in metabolism as "low-high-low-high" activity may relate to the transformation from gametophyte to sporophyte generation.

根据上述结果可以得出,栽培甜菜从卵细胞成熟→合子初期→合子休眠期→合子分裂前期→二细胞原胚的超微结构变化中多聚核糖体的变化最为显著,表现为&少→多→少→多&的数量变化过程,反映出细胞代谢状态也经历了&弱→强→弱→强&的变化过程,这种变化趋势与配子体世代向孢子体世代转变有关。

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