子叶的

Results as bellows: AtSIRT1 was located in Mitochondrial as hSIRT4 of human, and maybe take part in respiration and electron transformation chain, AtSIRT2 was located in nucleolus as hSIRT6 of human, maybe play important role in extend lifespan;mutation in AtSIRT1 leaded to cotyledon of plant turn to yellow and caused short life span. Mutation in AtSIRT2 could make the color of leaf turn to purple and accumulate a lot of anthocyanin;Sirtinol, a inhibitor of SIRT which did not cause the same model of the mutation of AtSIRT1 and AtSIRT2 indicated that the mechanism of Sirtinol was different from other organism;the structure of AtSIRT1 and AtSIRT2 were similar to other known Sir2, which indicated that they maybe have the same function;AtSIRT2 was overexpressed and its activity was detected.

结果表明，1，拟南芥AtSIRT1与人的同源蛋白hSIRT4相同，定位于线粒体，可能参与呼吸作用和电子传递，SIRT2与人的同源蛋白hSIRT6相同，定位于细胞核，可能同它的功能类似，在延缓衰老及调节细胞寿命方面起作用。2，AtSIRT1突变，可引起幼苗和植株的子叶变黄和早衰；AtSIRT2突变，可引起叶片发紫，沉积大量花青素。3，SIRT蛋白的抑制剂Sirtinol不能表型模写AtSIRT1和AtSIRT2突变体，说明Sirtinol在拟南芥中的作用机制不同于其他生物。4，AtSIRT1和AtSIRT2蛋白质结构预测表明与已知的Sir2蛋白相似，揭示其功能的相似性。5，在大肠杆菌中过量表达了其中一个基因(AtSIRT2)，可体外检测其酶学活性，进一步证明其功能。

Using nuclear DNA C-values for 539 angiosperms in China, we examined the variation of these values among growth forms and taxonomic groups and the relationship of these values with invasiveness. Mean DNA C-value of the 539 angiosperm species was 4.06 pg. Mean DNA C-value was(1) significantly lower for woody species (1.84 pg) than for herbaceous species(5.02 pg);(2) significantly lower for 360 dicots (2.20 pg) than for 179 monocots (7.80 pg);(3) significantly lower for annuals (2.78 pg) than for perennials(6.65 pg);(4) significantly lower for 134 weed species (1.93 pg) than for herbaceous non-weeds (6.75 pg) and for several families that have an unusually high proportion of weed species;(5) significantly lower for 47 exotic weed species (1.76 pg) than for 134 native weeds (1.93 pg), but significantly lower than that of "non-weedy" herbaceous species (6.75 pg);(6) lower for weeds than for "non-weedy" species in same genus or family; and (7) in herbaceous species, generally lower for weedy compared to "non-weedy" species, with some exceptions such as Avena fatua, whose DNA C-value is as high as 14.15 pg, contrarily, and some "non-weedy" herbaceous species in Cruciferae and Cucurbitaceae with very low values.

统计了中国境内有分布的539种被子植物的DNA C-值，分析了它们在不同分类群、生活型、倍性、生活史类型以及在杂草和非杂草类群中的分布情况，主要结果如下：(1)539种被子植物DNA C-值平均为4.06 pg，其中木本植物的DNA C-值平均为1.84 pg，低于草本植物的平均值(5.02 pg)；(2)双子叶植物(360种)的DNA C-值平均为2.20 pg，极明显地小于单子叶植物(179种)的平均值(7.80 pg)；(3)1年生植物的DNA C-值平均为2.78 pg，明显小于多年植物的平均DNA C-值(6.65 pg)；(4)134种杂草的DNA C-值平均为1.93 pg，明显小于非杂草草本植物的平均值(6.75 pg)，含杂草较多的科，平均DNA C-值相对较小；(5)统计的47种入侵杂草的DNA C-值平均为1.76 pg，略小于134种杂草的平均DNA C-值(1.93 pg)，极显著地小于非杂草性草本植物(6.75 pg)；(6)以科为单位，不同科的DNA C-值存在着极大的差异；(7)DNA C-值与染色体倍性的关系并不明显，但是，随着倍性的增加，基因组变小；(8)在同一科、属中，与非杂草相比，典型杂草的DNA C-值往往偏小；(9)总体上杂草或杂草性强的植物，它们的DNA C-值比非杂草性植物的要小。

Fruit dehiscent siliques or silicles, linear, oblong, ovoid, lanceolate, or ellipsoid, terete or latiseptate, sessile or shortly stipitate; valves with a distinct midvein, smooth or torulose; replum rounded; septum complete, membranous, translucent; style obsolete to 2.5 mm, persistent; stigma capitate, entire or rarely 2-lobed, Seeds uniseriate, wingless, oblong, plump; seed coat reticulate, not mucilaginous when wetted; cotyledons incumbent.

开裂的果长角果或短角果，线形，长圆形，卵球形，，或椭圆形，具宽隔膜的圆柱状或，无梗或具短柄；裂爿具一离生中脉，滤波或；假隔膜绕行；隔膜完全，膜，半透明；对2.5毫米废退，宿存的花柱；柱头头状，2裂的全缘或很少，单列的种子，无翅，长圆形，肥厚；网状，不黏的种皮被弄湿；子叶现任。

When the frequency of codon usage of citrus was compared to Homo sapiens, Drosophila melanogaster, Saccharomyces cerevisiac and Escherichia coli, we found that the codon preference is considerably different between citrus and non-plant species. While compared to Arabidopsis thaliana, Lycopersicon esculentum, Oryza sativa and Musa acuminata, we found that the codon preference was identical in dicotyledons, but was different between citrus and monocotyledon, Oryza sativa and Musa acuminate.

将柑橘的密码子使用频率与人、果蝇、酵母和大肠杆菌等不同种类模式生物比较后发现，柑橘密码子的偏爱性与不同种类生物有不同程度的差异；但将柑橘的密码子使用频率与拟南芥、番茄、水稻和尖叶蕉等不同种类的植物相比，发现柑橘密码子的偏爱性与同为双子叶植物的拟南芥、番茄完全一样，而与水稻、尖叶蕉这2种单子叶植物均有较大的差异。

DNA C-value is of practical value in the forecast of the invasiveness of exotic species. However, such predictions must be made with comparisons within families because the DNA C-values vary greatly between woody and herbaceous species, between monocots and dicots, between annuals and perennials, and especially among families.

DNA C-值在预测外来物种的入侵性方面具有一定的应用价值，但是，由于在木本植物和草本植物之间、单子叶与双子叶植物之间、一年生和多年生植物之间，特别是在不同的科之间，植物的DNA C-值较明显的差异，因此，根据DNA C-值预测外来物种的入侵性，应该严格地限于同一科内的相关物种间的比较。

To examine the hypothesis, we test whether maize beta-amylase can serve as a lipase inhibitor.In order to conduct the interaction assay of beta-amylase and lipase, we adopted a preparative electrophoresis method with a Prep Cell to purify beta-amylase proteins from germinating maize endosperms and soybean cotyledons. We pre-mixed tested proteins with lipid substrate prior to the addition of lipase preparation and assayed the lipid hydrolytic activity.

本实验利用Prep Cell纯化玉米萌芽谷粒和大豆子叶中的beta-淀粉酶，分别将两种不同物种的beta-淀粉酶与胰脂解酶预先反应后，再加入脂解酶反应液中观察活性的变化，结果发现脂解酶的活性未如预期，没有抑制的效果产生，将甘薯beta-淀粉酶以同样方法与胰脂解酶作用，结果同上述两种beta淀粉酶依旧没有抑制的现象。

Honghezi,Dongbi,Shieryuelongyan,etc.The results showed that the method of Calcium-alginate -embedded suspended culture shaking at50r/min was suitable for longan proto plast culture;and the optimal regeneration,with up to5%of microcolony formation,occurred when protoplasts from suspensions were cultured ...

研究结果表明，海藻酸钙包埋悬浮振荡培养(50r/min)是龙眼悬浮细胞原生质体培养的适宜培养方式，在含有多种生长调节剂的改良MS培养基(MP6)上，再生小克隆的形成频率可高达5%；采用龙眼胚性愈伤组织体胚诱导、成熟和萌发的优化方案，原生质体再生小克隆分化子叶形胚状体的频率达100%，萌发植株的频率一般大于45%。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。