子叶

Effect of 6-BA on regeneration of cotyledonary node including germination of seedlings, multiple shoots induction and shoots elongation from soybean cultivars "heinong 35" were studied.

以大豆品种黑农35的子叶节为材料，研究6-BA对子叶节再生三个阶段即无菌苗萌发、丛生芽诱导、丛生芽伸长的影响。

The maturation process of somatic embryos could be divided into four stages: ESM. globular and torpedo embryoid, pre-cotyledonary embryoid, mature cotyledonary embryoid.

体细胞胚的成熟过程大致可分四个时期：胚性胚柄团、球形胚至鱼雷形胚、子叶前期胚和子叶期成熟胚。

Xylem: Chinese P.chinensis Bge 49.15mg/g, Atlantic Ocean 46.58mg/g, UCB 55.28mg/g. 3.In certain condition, Gas-chromatograms and Mass spectrum are use to identifie phenol. Pistacia vera L .leave contain pyrocatechol 0.7640mg/g, pyrogallol 0.0580mg/g, gallic acid 1.03024mg/g, catechin 0.1040mg/g and coumaric acid by different pre-treatment methods.

采用不同方法提取阿月浑子叶片酚类物质，气相色谱-质谱法对其进行定性与定量分析，阿月浑子叶片中含有邻苯二酚0.7640mg/g；邻苯三酚0.0580mg/g；没食子酸1.3024mg/g；儿茶素0.1040mg/g，香豆酸；确定了最佳的前处理方法。

At present, regeneration system of cotyledons, hypocotyls, and stems, protoplasts and cotyledons codes have been established among Cucurbitaceae. There are several methods of transformation, such as agrobacteria -medidated genetic, particle gun, pollen tube passage, DNA soaking, ovary embryonary sac injection. Transgenic is tending to antivirotic and delayed maturity. Selection marker used often is NPT II.

目前，在葫芦科植物中已建立了子叶、茎尖、胚轴、茎段、原生质体和子叶节等的再生体系；用于遗传转化的方法有农杆菌介导法、基因枪法、花粉管通道法、DNA浸泡法等；用于转化的目的基因多为抗病毒病基因和延迟成熟的基因；NPTII基因为最常用的筛选标记基因。

And on the basis of this protocol,protoplast electrofusionof cabbage with radish was studied to find out the condi-tions and parameters affecting electrofusion.

本研究通过对甘蓝子叶、萝卜子叶和下胚轴原生质培养的研究，建立了甘蓝与萝卜原生质体培养再生植株体系。

The normal and abnormal embryoid were distinguished by the rate of normal plantlets induced and the regeneration modes of the cottons varieties were compared to two ways, one with the highest rate of normal inducement was the normal way of regeneration plantlet from callus, embryogenetic callus, global embryoid, heart-shape embryoid, torpedo-shape embryoid, cotyledon embryoid to normal plantlet, etc, the other with the lowest rate of normal inducement was the abnormal way from callus, embryoid callus, global embryoid-like, heart-shape embryoid-like, fish torpedo-shape embryoid-like, cotyledon embryoid-like, abnormal plantlet to normal plantlet, etc.

转化后采用平行实验得到不同品种的不同培养体系的愈伤组织、胚性愈伤组织、胚状体、类胚状体、畸形苗和正常苗，以最终正常苗诱导率高的体系的材料作为正常苗的发生过程，以诱导率低的作为畸形苗的发生过程，即分为正常苗的发生过程，愈伤组织——球型胚状体——心型胚状体——鱼雷型胚状体阶段——子叶型胚状体——正常苗：畸形茁的发生过程，愈伤组织——类球型胚状体——类心型胚状体——类鱼雷型胚状体阶段——类子叶型胚状体——畸形苗。

Cotyledons (3 or)4-8(-12). Germination epigeal. 2 n = 24*.

子叶(3或者)4-8(-12)。发芽时子叶出土 2n = 24×。

Cotyledons 4-9(-15). Germination epigeal. 2 n = 24*.

子叶4-9(-15)。发芽时子叶出土 2n = 24×。

They form the first photosynthetic organ in seeds showing epigeal germination, e.g. sunflower but in others they remain underground.

有些种子的子叶在种子萌发后露出土面，进行短期的光合作用，如向日葵，但在另一些植物中子叶仍然在地下。

Seed germination may be either epigeal, in which the cotyledons appear above ground, or hypogeal, in which the cotyledons remain below ground.

种子的萌发可以在地上—子叶出土萌发，也可以在地下—子叶留土萌发。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。