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WOX3 specifies the cell fate at the margins of cotyledonary primordia and regulates the rate of cell division in the margins of meristem in Arabidopsis.

阿拉伯芥的PRS/WOX3基因参与子叶原基边缘细胞专一性的决定,及调控分生组织边缘细胞分裂的速度。

Two-dimensional electrophoresis (2-DE) was used to study the variation expression of proteins of cucumber cotyledonary nodes induced with PP333. The characteristics and functions of differentially expressed proteins were analyzed with MALDI-TOF-TOF/MS and Protein Data Bank.

应用双向凝胶电泳技术研究PP333处理后黄瓜离体子叶节培养物差异蛋白的表达,结合质谱分析和蛋白质数据库检索,确定所检出差异蛋白质的性质和功能。

The different explants, hypocotyl, terminal bud, cotyledonary node, seedling were studied to induce bud or callus with different concentrations of 6-BA and to establish a better system for transformation.

利用大豆不同品种的下胚轴、顶芽、子叶节、原位再生等几种外植体进行诱导植株再生的实验,并通过添加不同浓度的6-BA来观察诱导愈伤组织和出芽的情况,为下一步的转化实验建立一个良好的组培平台。

C Cotyledonary somatic embryos had more morphological variation than zygotic ones. We achieved somatic embryogenesis of Manchurian ash for the first time.

体细胞胚与合子胚的发生程序基本相同,都经历了原胚、心形胚、鱼雷形胚和子叶胚这几个典型发育时期。

It was observed that a number of different polypeptides are phosphorylated in the five tested development stages including non-embryogenic callus,embryogenic callus,globular embryos, torpedo-shaped embryos,and cotyledonary and mature embryos.

在测试的体细胞胚发生的5个阶段(非胚性愈伤组织、胚性愈伤组织、球形胚、鱼雷胚和子叶成熟胚)观察到许多不同的多肽被磷酸化。

When the embryogenic calli were transferred to medium MS+5mg/L ABA+50g/L PEG+5mg/L AgNO〓 for one month, a lot of normal cotyledonary somatic embryos were produced.

当胚性愈伤组织转到MS+5mg/LABA+50g/L PEG+5mg/L AgNO〓的分化培养基上,1个月后可产生大量正常的子叶期成熟体细胞胚。

The expression profiles analysis of 25 genes belonging to 8 types in different development periods of Y35 somatic embry showed, genes involved in transcription, signal transduction, cell fate, substance transport, cell and development regulation were active at early and middle stages of somatic embryogenesis, while at the stage of cotyledonary embryo maturation, the high level-expressed genes were mainly those related to storage protein, maturation and defense.

通过半定量和Real-Time PCR技术,对WRKY和MAPKK等8类25个差异表达基因在Y35体细胞胚不同发育时期的表达分析显示,转录、信号转导、细胞生长分裂、细胞命运、物质转运和多数发育调节相关基因在体细胞胚胎发生的早期或中期比较活跃,而进入子叶胚成熟后期则主要是储藏蛋白、成熟和防御相关基因大量表达。

There were 2 peaks of starch accumulation in the process of somatic embryogenesis,the first time was at the stage of embryogenic callus,the second time was at the stage of globular embryoids;and a small raise appeared at the stage of torpedo-shaped embryoids,and the content of starch was slightly higher than those of heart-shaped embryoids and cotyledonary embryoids.With the development of embryos,the tendency of the changes of the content of DNA,RNA and the total nucleic acids was approximately similar,and the peak values were all at the stage of cotyledonary embryoids,and the change of the content of RNA was more remarkable than those of DNA and the total nucleic acids.The tendency of change of the content of proteins almost took on the S-shaped curve.

结果表明:龙眼胚性愈伤组织体胚发生过程中淀粉有2个累积峰值,第1次出现在胚性愈伤组织阶段,第2次出现在球形胚阶段,在鱼雷形胚阶段又有一个小回升,这时淀粉积累较心形胚和子叶形胚阶段高一些;随着胚胎的发育,DNA、RNA、总核酸含量的变化趋势大致相同,最高点均出现在心形胚阶段,其中RNA含量的变化较DNA、总核酸含量明显,蛋白质含量的变化曲线呈&S&形。

The results of changes of endogenous hormone contents at early stage of floral differentiation in cotyledonary nodes of cucumber seedlings after decapitation were as follows: The contents of IAA and ZT in cotyledonary nodes of the cucumber seedlings within 0~4 d after decapitation decreases while the level of GA3 first increases then declines within 1~3 d, after that it increases again.

对花芽分化初期子叶节中内源激素质量分数的测定表明,IAA质量分数在花芽分化初期处於较低水准,ZT质量分数呈下降趋势,GA3质量分数则出现升-降-升的波动状态。

Cotyledonary node explants were prepared from 5-7 day old seedlings, infected and co-cultivated with Agrobacterium tumefaciens. Resistant adventitious buds emerged after 4 weeks on selective medium.

从发芽5-7天的大豆无菌苗切取子叶节外植体,经农杆菌感染和共培养后,在选择培养基上4周左右出现抗性不定芽。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。