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Keywords : monocotyledonous crop;dicotyledonous crop;EST-SSR;transferability of primer

中文关键词:单子叶作物;双子叶作物;EST-SSR;引物通用性

(1)29,28,26 of 30 pairs of EST-SSR primers from wheat respectively had amplification products in maize,oilseed rape and soybean,the rate of amplification was 96.7%,93.3%,86.7%,respectively;12 of 30 pairs EST-SSR primers from wheat showed polymorphism in monocotyledons wheat and maize,9 in dicotyledons oilseed rape and soybean,and 4 in both monocotyledonous and dicotyledonous crops.

基金项目:国家生命科学与技术人才培养基地科技创新基金项目(2007088);国家油菜产业化项目科学家岗位"化杀岗位"(nycytx-00505);国家自然科学基金项目(30871578)中文摘要:探讨单、双子叶作物间EST-SSR引物和标记的通用性。选取30对小麦、8对油菜和14对白菜的EST-SSR引物,分别以10个小麦、10个油菜、5个大豆品种和5个玉米自交系的基因组DNA为模板,进行PCR扩增及扩增产物的多态性分析。

Comprising seed plants that produce an embryo with paired cotyledons and net-veined leaves; divided into six subclasses: Magnoliidae and Hamamelidae; Caryophyllidae (an early and distinctive offshoot); and three more or less advanced groups: Dilleniidae; Rosidae; Asteridae.

由生长双子叶胚胎和网状叶脉叶子的种子植物组成;分为六个亚纲;木兰群和金缕梅亚纲等。

The research advance on organogenesis, somatic embryogenesis and protoplast regeneration system of soybean are introduced in this paper.

终于在20世纪80年代初期有了转机,人们选择了幼胚、幼胚子叶或胚轴为外植体,用异常高浓度的植物生长调节物质,建立了大豆的体细胞胚胎发生和器官发生再生系统。

The invention relates to a rapid breeding method which uses MS as the basic culture medium and respectively performs the tissue culture of a xuancheng pawpaw through inducing a sprout and a root.

一种用MS作基本培养基,分别通过芽和根诱导进行宣木瓜组织培养快速繁殖的方法,其特征在于采用宣木瓜种子子叶或种子胚进行组织培养。

Eustele The STELE arrangement found in most gymnosperms and dicotyledons in which the vascular tissue is arranged into a ring of discrete bundles separated by medullary rays, all contained within a single ring of endodermis and pericycle.

真中柱:发现于大多数裸子植物和双子叶植物的中柱类型,其维管组织由不连续的,被髓射线分开维管束排列成环状。所有的微观组织都包含在内皮层和中柱鞘以内。

Are preparatively cultured in the differentiation culture mediums(IBA 1 mg/L, BA0.4 mg/L) for two days, and AS is added in preparative culture medium and MSo bacterial liquid that has been centrifugated. The extents of colouration are not identical.

影响瞬时表达的因素很多,本实验主要对四个因素进行测试:预培养天数(1天、2天)、不同激素配比的分化培养(IBA 1.0mg/L BA 0.4mg/L、IBA 0.2mg/L BA 2mg/L)、是否加入乙酰丁香酮、外植体类型(子叶、真叶、下胚轴、根)。

When the embryonic original cell divided to form multicellur proembryos, the cell wall of the external cell was thickened to form a clear cell boundary to separate from ambient cells that was a phenomenon of isolation The protoderm began to be differentiated in the period of globular embryoid, and the procambium differentiated clearly in period of torpedo embryoid.

正常的球形胚和某些畸形球形胚继续发育,在心形胚或鱼雷胚阶段又出现出畸形胚,可以分为:①无茎端生长点;②原形成层发育不正常;③外形畸形,如有的是连胚,有的呈指状,有的呈叶片状等等。畸形胚有的败育,有的能继续发育,在子叶胚阶段形成畸形的植株。

Seeds 1 or 2, not comose; endosperm bony; cotyledons broadly ovate or subcordate, radicle superior.

种子1或2,不具丛毛;胚乳多骨;子叶宽卵形的或近心形,胚根上位。

If redroot pigweeds are the only weeds the minimum dosage of herbicide can be used based on the recommendation.

在不同高度的麦茬环境中,苗后防除双子叶杂草,应使用大雾滴喷头。

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