子单元

An expression construct specified in mammary gland with double cistrons of human G-CSF gene and IRES-EGFP gene under control of ovine beta-lactoglobulin gene flanking sequence,has been constructed in two parts (named fragment I and Fragemnt II)that share an overlapping region of 2.2 kb sequence.Two sites of loxp and lox2272 for homologous recombination were inserted into both flanking regions of G-CSF.The lengths of fragment I and Fragment II are 5.9kb and 5.6 kb,respectively.The whole length of the expression vector (β-LG-hG-CSF-IRES-EGFP) is 9.3 kb.

以绵羊β乳球蛋白基因（β-Lactoglobulin，β-LG）为转基因表达框架，将人G-CSF(Human Granulocyte Colony Stimulating Factor， hG-CSF)基因与报告基因-增强型绿色荧光蛋白(Enhanced Green Fluorescenct Protein， EGFP)基因作为双表达单元拼接到β-LG基因的第一外显子处，并在G-CSF基因两侧引入同源重组位点loxP、lox2272，将打靶基因表达构件β-LG-hG-CSF-IRES-EGFP(总长9.3 kb)分为两段进行构建，片段Ⅰ（长：5.9kb）与片段Ⅱ（长5.6 kb）两段重叠部分为2.2 kb。

Full length LMP2A cDNA was firstly incised from pGEM-T-LMP2A with EcoR Ⅰ, Sma Ⅰ digestion, and then inserted into eucaryote. expression plasmid pCIcc controlled by CMV promoter. The CMV-LMP2A-SV40 expression unit was digested by ClaI, and inserted into E1-substituted adenovirus vector pAx1cw. Then the LMP2A recombinant adenovirus vector was cotransfected into 293 cells together with EcoT22I digested Ad5-TPC.

将带有LMP2A cDNA的重组质粒pGEM-T-LMP2A用EcoR Ⅰ、Sma Ⅰ双酶切下LMP2A cDNA，并将其插入含同样酶切位点的真核表达质粒pCIcc中，使其受控于CMV启动子下；用ClaI切下CMV-LMP2A-SV40表达单元，插入E1、E3区替代的腺病毒载体pAX1CW，选择正确的克隆pAX1CW-LMP2A与Ad5 DNA-末端肽复合体共转染293细胞，通过同源重组获得复制缺陷型的重组腺病毒(Ad5-LMP2A)。

"In the early 1970s it was discovered that these particles are made up of several types of even more basic units, named quarks, which, together with several types of leptons, constitute the fundamental building blocks of all matter."

1970年代初，发现这些粒子由几类称为夸克的更基本的单元组成。夸克与几类轻子一起组成了所有物质的基本结构单位。

"In the early 1970s it was discovered that these particle s are made up of several types of even more basic units, named quarks, which, together with several types of leptons, constitute the fundamental building blocks of all matter."

1970年代初，发现这些粒子由几类称为夸克的更基本的单元组成。夸克与几类轻子一起组成了所有物质的基本结构单位。

"In the early 1970s it was discover ed that these particles are made up of several types of even more basic units, named quarks, which, together with several types of leptons, constitute the fundamental building blocks of all matter."

1970年代初，发现这些粒子由几类称为夸克的更基本的单元组成。夸克与几类轻子一起组成了所有物质的基本结构单位。

Second, this paper systematically studies methods for impedance matching between dipole-type RFID tag antennas and tags ICs, which includes parasitical element loading, vee dipole technology and meander line loading technology.

第二，本文系统化研究了偶极子RFID标签天线与标签IC的阻抗匹配方法，包括寄生单元加载技术和弯折线加载技术。

Idlers use stamped bearing house. Using wide clearance bearing and multi-layer labyrinth seal improves service life, reduces rotation resistance and friction coefficient. Cast welding and keyless connection has been used for pulley, Hydraulic coupling and hard surface cone cylinder gear speed reducer for drive unit, angle type structure for head frame, so these improve starting performance, drive efficiency, load capacity and service life, reduce floor area and weight of equipment.

托辊采用了35°槽型前倾结构，辊子为冲压轴承座结构，大游隙轴承和册层迷宫密封，提高了寿命，降低了旋转阻力和摩擦系数，滚筒采用了铸焊结构和无健联接，驱动单元采用了液力耦合器和硬齿面圆锥圆柱齿轮减速机，头架采用角型结构，从而改善了启动性能，传动效率，承载能力和使用寿命，减少了占地面积和设备重量。

Module testing is a process of testing the individual subprograms, subroutines, or procedures in a program.

模块测试，或者说是单元测试，是单独测试子问题，子程序以及程序进程的一个流程。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。