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ObjectiveTo probe into the hyperplasy effect of different curcumine administered approaches for the splenic lymphocyte of mice.

目的探讨姜黄素3种不同给药途径对小鼠脾淋巴细胞增殖的影响。方法通过噻唑蓝比色法比较姜黄素3种给药途径对小鼠脾淋巴细胞增殖的影响。

MethodsThe effect of curcumine on the proliferation of spleen lymphocytes through three different administration routes were determined with MTT test.

结果姜黄素体内给药和含药血清能够促进小鼠脾淋巴细胞的增殖,体外实验证实姜黄素能够抑制小鼠脾淋巴细

Curcumine ramification has a good effect on the proliferation of K562, even better than curcumine, and might have the multiple inhibitory effect on the p210 initiated signal pathway.

姜黄素衍生物FM17较姜黄素有更强的抗增殖能力,对P210及其激活的信号途径也有较强的抑制作用。

Objective To observe the effects of curcumine on granulocyte macrophage colony stimulating factor expression and airway inflammation in asthmatic rats. Methods 36 male Sprague-Dawley rats were randomly divided into normal control, model group,curcumine group, 12 rats in each group.

目的 通过观察姜黄素对哮喘大鼠肺泡灌洗液总细胞数及嗜酸性粒细胞计数、肺组织粒细胞-巨噬细胞集落刺激因子表达的影响,探讨姜黄素对哮喘的治疗作用。

Curcumine could improve experimental hepatic fibrosis and down-regulated the expression of leptin and leptin receptor in liver, which probably contributed to one of the mechanisms about anti-fibrosis.

姜黄素能明显改善实验性大鼠肝纤维化,同时下调肝纤维化大鼠肝脏中瘦素以及瘦素受体的水平,这可能是姜黄素抗肝纤维化作用的机制之一。

Methods The rats were divided into model group(only CCl4 hypodermic),curcumine treatment group(CCl4 hypodermic and curcumine injected to abdominal cavity in low,medium and high doses)and normal group.The pathologic changes were observed by light and electron microscope.

将大鼠分为模型组(单纯皮下注射CCl4),低、中、高剂量姜黄素治疗组(皮下注射CCl4的同时,给予不同浓度的姜黄素腹腔注射)及正常对照组,应用光镜、电镜观察肝细胞的病理学变化。

Methods MTT assay was used to detect the inhibition of the proliferation of K562 cell in different time and different dose. The effect of curcumine ramification with different dose in different time on P210(superscript bcr/abl) initiated signal pathway was detected by western blotting analysis.

四甲基偶氮唑蓝法检测姜黄素衍生物不同时间点和不同的浓度对K562细胞增殖的抑制作用。western blot的方法分析姜黄素衍生物FM17不同浓度、不同时间点对P210激活的信号的影响。

Based on the changes in the spectrum characters of guest molecules producedthrough inclusion complex formation with cyclodextrins, we have carried out two aspects of investigation:First, the mechanisms for the host-guest recognition reactions have been studied, such as the interactions between 3-CD and curcumin, the main constituent of the rhizomes of the plant Curcuma longa, the recognition of p-CD towards rubidate, the effective component of the Chinese traditional medicine madder, and the reactions of p-CD towards the antileprotic drug dapsone and the antiinflammatory drug nabumetone: The formation of supramolecular complex between P-CD and guest molecule has been discussed and verified in virtue of multitudinous means such asultraviolet-visible absorption, fluorescence emission, infrared spectrogram and so on. The influence of addition of linear alcohols on the supramolecular system has been studied and the reason why the noncovalent interactions in the three-component system could result in a decrease in the apparent association constant of the inclusion complex has been revealed.

本论文基于环糊精对被包结客体光谱性质的改变,开展了两大方面的研究:β-环糊精对姜黄属植物根茎主要成分—姜黄素、中药茜草有效成分—茜草双酯、抗麻风药物氨苯砜及抗炎药物萘丁美酮的主-客体识别反应机制研究:借助超分子包合物的紫外吸收光谱、荧光光谱或红外吸收光谱等手段,通过多种方法探讨并验证了超分子包合物的形成;研究了直链醇的加入对超分子体系的影响,揭示了三元体系中非共价相互作用导致超分子包合物表观结合常数降低的原因:对超分子包结反应进行了热动力学研究,探讨了各热动力学参数的变化趋势并从熵焓互补的角度考察了超分子包结过程;通过荧光猝灭实验进一步揭示了水相中β-环糊精增敏客体荧光的本质原因,从而为超分子化学提供新的理论和研究方法。

Our results revealed that TGF-β1 at 1 ng/ml induced collagen deposition of HSC-T6 cells (145 ± 5 % of controls), and pre-exposure of cells to curcumin (1.25~10 μM) inhibited the stimulatory effect of TGF-β1 in a concentration-dependent manner. Curcumin (10 μM) significantly reduced the TGF-β1-induced collagen deposition (97 ± 7 % of controls) without cytotoxicity in the MTT assay .

我们的结果显示,1 ng/ml 浓度的TGF-β1可以有效地增加星状细胞对胶原蛋白的制造(达控制组的145 ± 5 %),而在预先投予浓度范围为1.25~10 μM的姜黄素后可对TGF-β1引起的刺激反应产生浓度效应的抑制作用;姜黄素在浓度10 μM下可明显地抑制TGF-β1引起的胶原蛋白沉积(达控制组的97 ± 7 %)而不致产生细胞毒性。

From time immemorial, with turmeric root turmeric powder made of orange food has been the main ingredient in curry sauce.

从远古时代开始,用姜黄根制成的橙黄色姜黄粉就一直是食品调料咖喱中的主要成分。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。