失神的

OBJECTIVE: To study the influence of the electric stimulation of denervated muscle atrophy.

目的 研究电刺激对失神经支配骨骼肌萎缩的影响。

The 72 special psychological health appraisement systems that constructed perfectly after classify, relationship analysis and structural test. They are: homicide, violence, sin, epilepsy, deliquium, schizophrenia, suicide, accident, danger, substantial, play, paranoia, hysteria, depressibility, cyclothymia, phobia, anxiety, obsessive, sexuality, diet, sleep, domestic, friendship, sociality, examination, study, interesting, illness, cognition, sense, temper, affection, emotion, sensibility, consciousness, will, attention, adaptability, unemptiness, enthusiasm, honesty, straight-out, trust, responsibility, stanchly, devotedly, optimistic, passion, unclouded, brotherliness, gregarious, mild, open, calm, toleration, self-control, self-esteem, continence, self-confidence, ability, efficiency, intellect, independence, mature, life-satisfaction, social- satisfaction, general-satisfaction, behavior-health, emotion-health, happiness, successfully, rhathymia.

通过归类、相关分析、结构方程检验，生成拟合完美的72个专项测评系统：杀人、暴力、犯罪、癫痫、失神、分裂、自杀、意外、遇险、物瘾、游戏、偏执、癔病、抑郁、躁狂、恐惧、焦虑、强迫、性欲、饮食、睡眠、相处、友谊、社交、考试、学习、志趣、病感、认知、感觉、性情、情感、情绪、意识、意志、注意、适应、充实、积极、诚信、坦诚、信赖、负责、坚强、务实、乐观、热情、开朗、友爱、合群、平和、豁达、坦然、宽容、自控、自尊、克制、自信、能为、有效、理智、自立、成熟、生活满意、人际满意、总体满意、行为健康、身体健康、情绪健康、快乐幸福、心想事成、无忧无虑。

The sequence of poems began with the Song of the Torches , which tells in a vision how a spiritual army will liberate men and women whose feelings have been deadened, whose spirits have been confined, and on whose mouths "there is carven a hopeless despair".

下面这个系列的第一首是《火炬之歌》，它描绘了这样的景象：精神的力量终将使那些"情感像冰一样凝固的/血像冰一样冷的/嘴角雕着失神的绝望的"人们，获得自由。

BACKGROUND: Nuclear factor κB/ muscle RING finger protein1 (MuRF1) pathway is one of the most important molecular mechanisms in skeletal muscle atrophy.

摘要背景：在失神经骨骼肌萎缩中，核因子κB /MuRF1通路是最关键的分子机制之一，抑制该通路可以提高失神经骨骼肌的力量，保持肌肉数量和促进肌肉再生。

William looked out of sorts, I said "I love her injury."

威廉望了望失神的我，说道"是我的爱伤害了她。"

The unoperated sides of the treated animals also served as controls. Six normal rats were treated as normal control group. Three different siRNA plasmid solution containing RC2-Ⅰ, MAFbx-Ⅱ, CON (50μl , 0.8μg/μl)was injected and transfected by electroporation as methods mentioned above, respectively. The changes of RC2 and MAFbx mRNA levels and RC2 protein levels after 3 days were determined by real-time quantitative PCR and Western blot, respectively. On postoperative 2, 3 and 4 weeks, the rate of wet muscle weight preservation, mean diameter of muscle fiber and mean cross-section area of muscle fiber and muscle protein content were checked and then compared between group CON and group RC2 or group MAFbx, respectively. The differences between groups were analyzed by one-way ANOVA. Ultrastructural changes of muscle fiber were observed at 2, 3, 4 weeks postoperation.Results GFP plasmid was efficiently deliverd into muscle by electroporation and robust GFP expression in muscle could be observed more than three weeks. Histology shows that injected plasmid DNA diffuses extensively in muscle tissue.

1、健康雌性SD大鼠18只，随机分为电穿孔组和非电穿孔组，每组9只，制作右下肢趾长伸肌失神经支配模型；EP组为将质粒pEGFP-N1溶液50μl(0.8μg／μl)注射入右趾长伸肌后，立即于两侧腱腹交接处给予电穿孔，电穿孔参数为：电场强度为200V／Cm，脉冲100μs，频率1Hz，施加10次脉冲；NEP组仅质粒pEGFP-N1溶液注射；转染后1、2、3周，荧光显微镜下观察趾长伸肌中GFP的表达情况，转染后1周行Western印迹检测趾长伸肌中GFP蛋白的表达情况，检测和优化体内转染效率。2、健康雌性SD大鼠78只，随机分为失神经对照组、RC2基因治疗组(RC2组)，MAFbx基因治疗组，每组24只，制作右下肢趾长伸肌失神经支配模型，余6只为正常组；分别将含CON、RC2、MAFbx基因的siRNA重组质粒注射入趾长伸肌，之后给予电穿孔，方法同上；治疗后3天实时定量PCR和Western印迹检测各组中RC2或MAFbx基因的mRNA和蛋白的表达变化，治疗后2、3、4周检测各组肌湿重维持率、肌细胞直径和肌细胞截面积，肌细胞超微结构变化以及肌纤维中蛋白含量变化。

Result :There was no significant difference between sexes,ages and sides.The fibrillation potential amplitude was maximum at 3 to 4 months after denervation of the posterior cricoarytenoid muscle or at 5 to 6 months after denervation of the thyroarytenoid muscle and still remained at certain level for years in some patients.Significant difference was showed in complete and incomplete nerve injuries groups,but changes of amplitude with time in incomplete nerve injuries group were small.

结果：不同性别、年龄及不同侧的纤颤电位波幅间的差异无显著性，失神经环杓后肌纤颤电位波幅均值在病程2～＜4个月时最高，而失神经甲杓肌纤颤电位波幅均值在病程4～＜6个月时最高，相当一部分患者的纤颤电位波幅晚期仍维持一定水平，并且神经完全损伤者和不完全损伤者各时间纤颤电位波幅均有显著性差异，而神经不完全损伤者各时间幅度变化小。

The morphologic change of muscle fibers: At the early stage of denervation, the area of cross sections decreased, some muscle fibers turned into angular fibers, nuclei congregated around the center of the fibers. After 8 weeks of denervation, atrophic and hypertrophic fibers coexist. Collagen increases under the endomysium and between the fibers.

肌纤维的组织形态学变化：面部肌肉失神经支配早期，肌纤维的横截面面积变小，出现角状纤维，肌细胞核聚集，细胞核从肌纤维边缘移至肌纤维中央附近；失神经支配后期，肿胀与萎缩的肌纤维并存，肿胀的肌纤维的横截面成倍大于正常的肌纤维，而萎缩的肌纤维的横截面则成倍小于正常的肌纤维；有大量的角纤维存在，肌纤维之间有较多的结缔组织增生。

OBJECTIVE: To explore the expression of NF-κB and MuRF1 in denervated skeletal muscle atrophy in rats and the effect of losartan on NF-κB / MuRF1 pathway.

目的：探讨核因子κB、MuRF1在失神经骨骼肌中的表达及氯沙坦对核因子κB/MuRF1通路的影响作用，以期寻找延缓失神经骨骼肌萎缩的新途径。

On his estate at Neverland in southern California, remote from the "normal people" who might grab and scratch him, he lived like a child with blank-eyed mannequins, pet snakes and Ferris wheels.

当他居住在南加州自己的梦幻庄园中时，远离了那些会对他搜刮攫取的&普通人&，象孩童一样地生活着，伴随他的是茫然失神的塑料模特、宠物蛇以及费里斯摩天轮。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。