大鼠

Results: During the development of spinal cord. NIDD mRNA was highly expressed at embryo 16 d and at postnatal 1 d, and co-localized with nNOS in the undifferenti ated ventral horn, as well in white matter. And then it decreased significantly till postnatal 12w. nNOS mRNA was highly expressed from postnatal 1 d to 3 d. The high expression of NIDD mRNA appeared at the 8 hour after spinal cord injury, and co-localized with nNOS in neurons of ventral horn, intermedial zone, the area around central canal and dorsal horn, and recovered to the normal at dry 7 day after SCI.

结果：大鼠发育过程中，胚胎16 d的大鼠脊髓中可见NIDD mRNA的高表达，在生后1d，与nNOS共表达于尚未分化成熟的前角，在白质也见NIDD的阳性信号成年后呈低表达；nNOS mRNA于生后1~3 d出现表达高峰；脊髓损伤后NIDD mRNA表达明显增多，在8 h到达高峰，分布于脊髓前角，中间带、中央管周围及后角nNOS阳性的神经元，7 d恢复至正常水平；nNOS mRNA在损伤后8 h达到高峰，1 d降低至正常水平。

It was observed that, at the doses of 50 mg/kg and 100 mg/kg, geniposide could significantly increase the biliary secretion after intraduodenal administration, while crocins showed no effect.

结果显示西红花苷50和100 mg/kg剂量均不增加大鼠的胆汁流量，而京尼平苷50和100 mg/kg剂量均可显著增加大鼠胆汁流量，降低胆汁内胆固醇含量，增加胆汁内HCO－3浓度，但对胆汁酸，胆红素，〔Ca2＋〕含量无显著影响。

2Compared with PN group,following microcirculatory changes were observed in EEN group in vivo status:interlobular artery and vein structures disappeared,the vessel course was distorted and deformed;intralobular vessels were twisted into nidus,there was patchy bleeding around the vessel combined with necrosis;microcirculatory structures of pancreatic island disappeared and pancreatic tissue necrotized;capillary network had bleeding and necrosis.

2活体状态下观测大鼠胰腺微循环情况显示与PN组相比，接受EEN治疗大鼠急性胰腺炎微循环小叶间动、静脉伴行结构消失，血管走行扭曲变形更加明显；小叶内血管扭曲变形成团，血管周围出现斑片状出血，并伴有坏死；胰岛微循环结构消失，组织坏死；毛细血管网大片状出血、坏死。

Methods Wistar rats were injected intramuscularly continually with dexamethasone to establish the rat model of PCP.

方法用地塞米松连续肌肉注射Wistar大鼠，诱导建立PCP大鼠模型。

Methods Seventy-eight adult female PVGrats were divided into experimental group (n=60) and control group (n=18), intraneural injection technique was used to supply three kinds of NO donors with different half-life(DETA NONOate, DPTANONOate and Spermine NONOate) or saline into the rat sciatic nerve subperineurally under light microscope. At the 1th,2th,3th,7th,and 14th day of duration after sciatic nerve injection, a segment of injected sciatic nerve was taken out and embedded it in TAAB resin after tissue processing. Then it was cut into thin layers (1μm) and the histological changes were observed under high power microscope, meantime the number of degenerated axons were counted.

选择78只雌性PVG大鼠，分为实验组和对照组，以在体神经内微注射实验方法，将三种半衰期不同的NO供体(DETA NONOate，DPTA NONOate和Spermine NONOate)及生理盐水分别注入大鼠左侧坐骨神经的神经束膜下，于注射后的不同时间(1天、2天、3天、7天和14天)取出注射部位的坐骨神经，经组织学处理后做成树脂包埋块，以超薄切片机切片，然后在高倍显微镜下观察并计数神经横断面的变性轴突数目。

The anti-VEGF antibody group was treated with anti-VEGF polyclone antibody 300ng per 3 days by intrapelvic cavity. The LuoShiNeiYiFang group was filled with LuoShiNeiYiFang decoction per day, the concentration of this decoction is four times to human being.

种植学说是目前内异症形成的主要学说，采用大鼠内膜移植模型，我们观察活血化瘀中药干预对大鼠模型全血N0、iNOS和内膜组织VEGF表达的影响。

CaN Aβ gene silencing can reduce myocardial hypertrophy in cultured cells, si1280 (21bp) of CaN Aβ gene is the most effective target site for siRNA. The method of intrapericardial injection of plasmid, microbubbles and erzymes can improve transfection efficiency of non-viral plasmid with satisfying targeted transfection. But the scope of transfected myocytes is still limited. CaN Aβ shRNA expressing plasmid transfection in vivo by pericardial injection results in decreased CaN Aβ protein expression of small part of myocytes, and CaN Aβ mRNA only shows decreased trend. The dosage of non-viral vector and the parameters of ultrasound energy should be optimized in further study.

结论RNAi技术抑制CaN Aβ基因表达可有效减轻Ald诱导的心肌细胞肥大程度；CaN Aβ基因中si1280(21bp)片段为实现RNAi的更有效片段；微泡+酶类心包腔内注射超声导入的方法可有效改善非病毒载体在体心肌的转染效率，同时具有一定的靶向性，但总的转染范围仍然有限；采用这一方法进一步进行&一肾一夹&心肌肥大模型大鼠在体心肌细胞的CaNAβ的RNAi干预，发现心肌肥大大鼠心外膜下局部心肌细胞CaN Aβ蛋白水平降低，CaN AβmRNA水平虽有下降趋势，但无统计学差异，提示质粒的用量及超声导入的参数有待进一步研究使其优化。

Methods: The experimental silicosis model in rats was established by intratracheal instillation of silica.

建立大鼠矽肺模型，分别在染尘后第1、8、15、22、29、36、43、50、57和64d各处死3只试验组和1只对照组大鼠。

In normal rats,the pharmacological bioavailability reached 6.0% by inhalation and 11.4% by intratracheal delivery.

其药理生物利用度在正常大鼠体内分别为 6 。0 %和 11.4%，在糖尿病大鼠体内分别为 5 。6 %和 8.7%。

Except for control group, rats in the other groups were intratracheally administered with bleomycin. Animals in pulmonary fibrosis model groups were sacrificed on day 3, 7, 14, 28 and 56. The sections of the right lung were stained by HE, Masson and sirius red.

除N组外，其余各组采用气管内注入BLM致大鼠肺纤维化模型，分别于3、7、14、28、56 d处死各组大鼠，右肺行苏木精-依红、Masson胶原及天狼猩红染色，测定左肺羟脯氨酸的含量。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。