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METHODS The animal model was induced by injecting suspension of yeast hypodermically.

方法用酵母混悬液复制发热大鼠模型,采用实时荧光定量聚合酶链反应检测大鼠下丘脑中COX-1、COX-2的mRNA表达的变化。

To explore the influence on the expression of orexin A in the hypothalamus and orexin type 1 receptor (OX1R) in the medulla of neonatal rats after prenatal nicotine exposure, the 20 pregnant rats were divided into 2 groups randomly. Either nicotine (6 mg/kg per day) or normal saline solution (as same volume as nicotine group) was administered into the pregnant rats hypodermically from the fifth day to the end of gestation.

为了观察出生前尼古丁暴露对新生大鼠下丘脑orexin A及延髓内orexin 1型受体(OX1R)表达的影响,本实验将20只雌性成年大鼠随机均分为二组,怀孕后第5 d开始每天分别皮下注射尼古丁6 mg/kg或等量的生理盐水,直至分娩。

The model of rat liver fibrosis was induced with compound factors of 40%CCl4-olive oil liquor injection hypodermically(2 time per week,six weeks),high fatty and low protein foods.Meanwhile the rats were given equivalent dosage of correspondent drug(1 time per day,six weeks).At the end of six weeks.All rats were executed and left liver tissue and serum.

用40%CCl4橄榄油溶液和高脂低蛋白饮食复制肝纤维化大鼠模型造模,同时予等效剂量健脾软肝方及拆方灌胃,并以鳖甲软肝片为对照,每天一次,6周末处死,留取大鼠血清、肝组织。

Hematocrit and blood viscosity at shear rate in 150/s, 30/s, 5/s, 1/s, renal fibrosis serum markers, serum creatinine and blood urea nitrogen were detected respectively in sham operation and model group; microcirculation microscope was used to observe the thread of hypoglottis and auricle in rats.

建立单侧输尿管梗阻致大鼠肾间质纤维化模型,观察肾间质纤维化大鼠全血粘度150/s切变率、30/s切变率、5/s切变率、1/s切变率、红细胞压积、舌下及耳廓的脉络变化,同时检测肾纤维化指标、血清肌酐及尿素氮。

Exhausted exercise does harms to our immunologic function, and people can not benefit from it.

在这些指标的变化上,雌性大鼠和雄性大鼠未见显著性差异。

METHODS: Thirty six SD female rats were randomly separated into 3 groups (n=12 in each one) according to the infarction duration (1, 3, 28 d), which were randomly and evenly again separated into 2 subgroups: operated group and sham operated group. The levels of mRNA expression of ACE2 in the noninfarct and infract area of left ventricle 1, 3, 28 d after AMI were detected by RT PCR and analyzed semi quantitatively by image analysis system.

将36只SD雌性大鼠按时间段随机分为3组(每组12只),每组再随机分为假手术组、手术组2个亚组(每组6只),应用RT PCR方法检测大鼠AMI后1,3,28 d左心室梗死区及非梗死区心肌ACE2 mRNA的表达,并以图像分析系统对其进行半定量分析。

METHODS: Thirtysix SD female rats were randomly separated into 3 groups (n=12 in each one) according to the infarction duration (1, 3, 28 d), which were randomly and evenly again separated into 2 subgroups: operated group and sham operated group. The levels of mRNA expression of ACE2 in the noninfarct and infract area of left ventricle 1, 3, 28 d after AMI were detected by RTPCR and analyzed semiquantitatively by imageanalysis system.

要领:将36只SD雌性大鼠定时间段随机分为3组(每组12只),每组再随机分为假手术组、手术组2个亚组(每组6只),应用RTPCR要领检测大鼠AMI后1,3,28 d左心室梗死区及非梗死区心肌ACE2 mRNA的表达,并以图像阐发体系对其举行半定量阐发。

Their different part of denture were ground artificially to establish an animal model of infraocclusion.

采用人工磨低大鼠不同牙列段牙齿的方法建立低位咬合动物模型,于干预后1,2,4wk后将各组分别处死大鼠4只。

Methods The new method made use of suprahepatic inferior vena cava instead of pulmonary artery of the donor heart for the anastomosis to infrarenal vena cava of the recipient.

本方法利用大鼠供体大鼠肝脏以上下腔静脉代替肺动脉与受体肾以下腔静脉吻合。

In addition, MSCs from rat bone marrow marked with Hoechst 33342 were injected intocaudal vein, abdominal cavity and inguen of rats for observing the safety of rat bone marrowmesenchymal stem cells in vivo transplant.

另外,用Hoechst 33342标记的大鼠骨髓MSCs(剂量为:10~6个细胞/只)分别对大鼠进行全身注射、腹腔和腹股沟接种,对MSCs体内接种的安全性进行初步试验。

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