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METHODS:The distribution changes of N CAM at flatfish muscle neuromuscular junction of rat in its embryon phase,14 day after birth,adulthood and 10,20 and 30 days after its L5 lumber nerve root injury were observed by immunohistochemistry and confocal laser scanning microscopy technologies.

采用免疫组织化学方法和激光共聚焦显微扫描技术,观察了大鼠胚胎期,出生后14d,成年期以及L5神经根受压后10,20,和30d,大鼠比目鱼肌神经肌肉接头部N-CAM的分布变化。

There was no expression of CTGF protein in the pulmonary arteriole of the normal group, whereas, it was highly expressed in the tunica adventitia, slightly expressed in the endangium and rarely expressed in the tunica media of pulmonary arteriole in the hypoxemic group.

正常对照组大鼠肺小动脉壁CTGF蛋白未表达;低氧组大鼠肺小动脉壁外膜CTGF明显表达,内膜略有表达,中膜几乎无表达。

Aims: To establish a model of rat endotoxic pulpitis.

目的:建立大鼠实验性牙髓炎模型;原位观察大鼠正常牙髓和炎症牙髓中Bcl-2和Bax的表达情况,探讨两者在牙髓炎病理变化中的作用。

Spinal cord injury models were made in 60 wistar rats and the dynamic expression of bromodeoxyuridine and polysialylated ependymal cells adhesion molecule were determined by immuno-histochemisty.

方法]Wistar大鼠60只,制作大鼠脊髓全横断损伤模型,根据伤后不同时间点分组,应用免疫组织化学方法动态检测脊髓内5-溴脱氧尿嘧啶、多唾液酸神经细胞黏附分子的表达。

Appetite , expression , hair color ,etc.: Compared with the group of normal rats, from the 2nd week when making the mould, the rats' appetite of each modle group has been reducing in various degree ,the eyes does not sparking any more, the weight dropping , piloerection , epilate , and the fur is owed the gloss , hunchbacked , languid , slow in reaction, the activity reduces, it is apt to irritabling etc. aggravate continuously.

食欲、神态、毛色等:与正常组大鼠相比,从造模后第2周开始,各模型组大鼠食欲均有不同程度的降低,出现了双目无神、体重下降、竖毛、脱毛、及皮毛欠光泽,并有弓背、倦怠、反应迟钝、活动减少,易激惹、急躁等症状出现,并持续加重。

Cauterizing the episcleral vessels to build chronic elevated IOP can result in the decrease of stained grains in RGCs nucleus.

1。采用Akira法,烙闭大鼠右眼上巩膜静脉,制作大鼠持续性高眼压模型可导致RGCs细胞核内银染颗粒减少。

A diode laser with wavelength of 532-nm was used to burn the trabecular meshwork and episcleral veins of right eyes at 45 SD rats, while the left eyes were referred as control.

SD大鼠45只,麻醉后使用532二极管激光行右眼角膜缘360°光凝,角膜缘激光斑为80~100个,大鼠角膜缘颞侧、颞上及颞下巩膜浅层静脉3条,每条静脉光凝3~4个斑点,功率

To investigate the role of gene Gli3 in idiopathic congenital talipes equinovarus, we constructed the Gli3 luciferase reporter gene expression vectors to analyze the promoter activity of the rat gene Gli3. The regulatory element in the promoter region of the rat Gli3 was predicted using P-Match software and further verified by ChIP experiment.

为了探讨人类GLI3基因在单纯性马蹄内翻足(Idiopathic congenital talipes equinovarus, ICTEV)发生时所起的作用,文章构建了大鼠Gli3基因启动子区域荧光素酶报告基因表达载体来分析Gli3基因启动子的活性,并利用P-Match软件预测大鼠Gli3基因启动子区域可能的调控元件,应用ChIP实验加以验证。

Deference was significant. 2. Levels of serum TC, TG and LDL-C in D group were higher than those in C group. Difference was significant. Levels of serum TC, TG and HDL-C in ErD group were higher than C group.

血脂代谢:D组大鼠TC、TG、LDL-C均高于C组,差异具有显著性;ErD组大鼠TC、TG、HDL-C均高于C组,差异具有显著性;LDL-C水平:D组>ErD组>C,三组之间差异均具有显著性。3。

For those in control group (n=14), the animals did not undergo any surgery of the nerve fibers and ganglia. In Vi group (n=9), the animals underwent bilateral resection of the nasociliary and postganglionic parasympathetic nerve fibbers. In SCG group (n=8), the animals underwent bilateral resection of supper cervical ganglia. In V1+SCG group (n=9), the animals underwent both surgeries as Vi and SCG group did. In sham operation group (n=11), the animals underwent carotid manipulation with blunt-tipped forceps as well as dissection of nasociliary and postganglionic parasympathetic nerve fibers around the ethmoidal foramen, without cutting any nerves. SCS was performed on rats and CBF was monitored by laser Doppler flowmeter recordings.

SD大鼠分5组:对照组(n=14,不对神经纤维和神经节作任何手术处理)、V1组(n=9,切断双侧鼻睫状神经及其副交感节后纤维)、SCG组(n=8,切断双侧颈上神经节)、V1+SCG组(n=9,切断双侧鼻睫状神经及其副交感节后纤维和双侧颈上神经节)、假手术组(n=11,手术暴露双侧鼻睫状神经及其副交感节后纤维和双侧颈上神经节,但不切断神经纤维或神经节);给予大鼠SCS,并用激光多普勒实时记录脑血流。

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