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Objective To make an Achilles tenotomy rat model to observe the change in soleus weight and expression of myosin heavy chain isoform mRNA, and to find some clues for elucidating the mechanisms of soleus disuse.

目的建立大鼠跟腱切断模型,观测并比较跟腱切断与尾部悬吊大鼠比目鱼肌重量与肌球蛋白重链异构体 mRNA表达的变化,为探讨骨骼肌废用性萎缩的机理提供实验线索。

Results Compared with control group,the level of serum testosterone in medol group was increased obviously(P.01),the expressions of TGF-β1 in the endometrium and myometrium were decreased significantly(P.01), the expression of TGF-βRⅠ in myometrium was decreased obviously(P.05),while the expression of TGF-βRⅠin endometrium was increased significantly(P.05). In model group ,myometrium of the uterus was tenuis,endometrium was atrophic and the number of glandular organs was decreased.

结果:与正常组比较,ASR模型组大鼠血清T水平明显增高(P.01;子宫内膜、肌层TGF-β1表达量明显降低(P.01);肌层TGF-βRⅠ表达量明显降低(P.05),而内膜TGF-βRⅠ表达量却明显升高(P.05);与正常组比较,ASR模型组大鼠子宫肌层变薄,内膜萎缩,腺体减少。

Objective To explore the effect on the capability of antioxidizing in testis of male rats.

目的 探讨乙酰甲胺磷对雄性大鼠睾丸组织抗氧化能力的影响。方法 24只健康成年SD雄性大鼠,随机分为4组,每组6只。

Objective To explore the effect on the capability of anti-oxidizing in testis of male rats.

目的 探讨乙酰甲胺磷对雄性大鼠睾丸组织抗氧化能力的影响。方法 24只健康成年SD雄性大鼠,随机分为4组,每组6只。

Experimental research indicates:QZZGX granule can treat alcoholic fatty liver and DL桬thionine aminoacid fatty liver of the mouse by adjusting metabolism of fat, descending the level of TG,Gn in the liver, improving liver function, anti梠xidat ion descending the content of oxyproling hydroxyproling and improving the level of NO in the body.

实验研究表明:该药对酒精性和乙硫氨酸脂肪肝大鼠通过调节脂肪代谢、降低肝内TG、Gn水平、对酒精性脂肪肝大鼠改善肝功能、抗氧化、降低羟脯氨酸含量和改善体内一氧化氮水平等途径达到治疗脂肪肝的目的。

INTERVENTIONS: 4 vessel occlusion(4VO) brain ischemic models in rats stained with thionine staining and GFAP immunohistochemistry staining. were used. Sixty-four rats, of which bilateral vertebral arteries were occluded permanently by electrocautery, were divided into the following 8groups: sham operation group, cerebral ischemic preconditioninggroup, ischemic insult group; BIT group; MTPG + sham operation group;MTPG+BIT group; MTPG+ischemia group and -4C3HPG+BIT coup. All the rats were killed 7 days after the operation or the final ischemic treatment. Cerebral sections were selected and stained with thionine staining and GFAP immunohistochemistry staining.

干预:采用大鼠四血管闭塞全脑缺血模型,应用硫堇染色和胶质纤维酸性蛋白免疫组化法。64只大鼠椎动脉凝闭后分为假手术组、单纯预处理组、单纯缺血组、脑缺血耐受组,MTPG+假手术组、MTPG+脑缺血耐受组、MTPG+缺血组和-4C3HPG+脑缺血耐受组,所有动物均在手术后或末次缺血后7 d处死取材,行脑组织切片,硫堇染色和胶质纤维酸性蛋白免疫组化染色。

The influence of thyroxine on the healing rate of gastric ulcer.The experimental hypothyroidism in rat was achieved by giving 0.1% thiouracil solution as drinking water which led to the retardation of ulcerous healing.

甲状腺素对胃溃疡愈合率的影响:用0.1%硫氧嘧啶水溶液饮喂大鼠,引起大鼠实验性甲状腺功能低下,使胃溃疡愈合速度明显减慢。

Results Hearing threshold of both groups become worse with aging, and the treatment group showed the better auditory sensitivities, the less concentrations of MDA and the less frequency of mtDNA^4834bp deletion compare with the placebo group.

结果 (1)大鼠11月龄时,对照组与实验组的ABR反应阈为(49.3 3.9 )dBSPL与(49.7 4.4) dBSPL(P>0.05),耳蜗中丙二醛含量为(10.8 1.7)与(11.6 2.1) pmol/g,P<0.05;耳蜗内mtDNA4834bp缺失的发生率比较,差异无统计学意义(P>0.05);(2)大鼠24月龄时,对照组与实验组的ABR反应阈、耳蜗中丙二醛含量及耳蜗内mtDNA4834bp缺失的发生率比较,差异有统计学意义(P<0.01、P<0.01和P<0.05)。

MethodsSD rat models by modified Allen s assay for acute spinal cord injury were adopted. Motor nerve function of rat lower extremities in sCR1 group and normal saline group were evaluated by the tiltboard experiment. C3c, C9and CD59 positive expression sites and time phase in injury tissue at 12 h, 1, 3, 7 and 14 d after injury in the two groups were observed and the differences between the two groups were noticed.

方法采用改良Allen重物打击法制成SD大鼠脊髓急性损伤模型,采用斜板实验评定sCR1组与生理盐水组大鼠下肢运动神经功能,观察两组在伤后12 h,1,3,7,14 d时间点损伤组织C3c,C9及CD59阳性表达的部位及时程,并比较组间差异。

Fabrication of tissue section:after spinal cord injury ld,3d,5d,8d,14d,21d,we sacrificed rats in batch.4% formaldehydum polymerisatum were pured into the cardiac muscle and fixed for 24h.Then obtain the specimen of spinal cord from T_5 to T_(13).Establishing slices,HE dyeing,bcl-2 dectation,terminal doxynucleotidyl transferase-mediated dUTP nick end labeling.On the fourteenth day after spinal cord injury,we detect Gale grading score and tiltboard experiment.

组织切片的制备:脊髓损伤后1d,3d,5d,8d,14d,21d共6个时间段分批处死动物。4%多聚甲醛心肌灌注固定24h,切取每只大鼠T_5~T_(13)节段脊髓组织,常规制成切片,给于HE染色,Bcl-2检测,TUNEL原位末端标记以及伤后第14天检测Gale评分和斜板实验,检测大鼠脊髓损伤后细胞凋亡和脊髓神经功能恢复情况。

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