大鼠

Results: The changes following hydrocortisone intervention of rats could be identified by PCA, and the kidney deficiency syndrome rats entered a state of "hyperfunction", involving a series of changes in metabolic pathway and metabolic network. The response integral area of lactic acid (δ 1.37) increased, indicating the accumulation of metabolites of lactic acid. The amount of dimethylamine (δ 2.72) also increased, indicating that the disorder of kidney function was related to kidney-deficiency syndrome. The response of creatinine (δ 3.87), asparaginic acid (δ 2.83), taurine (δ 3.44, 3.28), and hippurate (δ 7.84, 7.56, 7.64, 3.97) all decreased significantly, indicating that the hydrocortisone might cause adrenal cortex excretion injury. The response integral area of succinate (δ 2.41) and citric acid (δ 2.53, 2.68) decreased, which was usually caused by disorder of the mitochondrial function.

结果：PCA方法处理肾虚证和空白对照大鼠尿液数据显示，两组大鼠的数据可以在得分图实现分类，与对照组比较肾虚组大鼠由于氢化可的松诱导，大鼠机体进入一个过消耗后衰弱的状态，相关代谢发生显著变化，乳酸(δ 1.37)代谢发生堆积；二甲胺(δ 2.72)的含量增加，提示肾虚与肾功能异常是密切相关的；天冬氨酸(δ 2.83)，牛磺酸(δ 3.44， 3.25)，马尿酸(δ 7.84， 7.56， 7.64， 3.97)，肌氨酸(δ 3.87)等的相对积分面积明显下降，这预示糖皮质激素可能引起了肾上腺皮质分泌功能的损害；琥珀酸(δ 2.41)和柠檬酸(δ 2.53， 2.68)是三羧酸循环能量代谢及糖酵解的中间产物，其含量的降低，通常是由于线粒体功能紊乱所引起。

MethodsNonheparinized autohemic blood of the rat was taken out from rat"s femoral artery and injected into its caudate nucleus immediately to establish a model of intracerebral hemorrhage.The changes of edema in the ipsilateral bassal ganglia , ipsilateral cortex, contralateral bassal ganglia and contralateral cortex were measured at 1,2,3,5 and 7 days respectively after intracerebral hemorrhage. The rat"s packed red blood cells, lysed red blood cells and hemoglobins at different concentrations were injected respectively into the rat"s caudate nucleus. The changes of edema in various regions of the brain at different time were measured as above. 5U thrombin was injected into the rat"s caudate nucleus and the changes of edema in various regions of the brain at different time were measured as above.

自体股动脉取血，并采用自体未肝素化新鲜血回注法建立大鼠尾状核出血模型，观察脑出血后1，2，3，5，7天血肿同侧基底节区、血肿同侧对应的皮质区、血肿对侧对应的基底节区及血肿对侧对应的皮质区脑组织水分含量的变化；分别以自体浓缩红细胞(PRBC，50％)、溶解红细胞(LRBC，50％)、不同浓度的大鼠血红蛋白注入大鼠尾状核，并观察不同时间不同部位脑组织水分含量的变化；以5U凝血酶注入大鼠尾状核，观察不同时间不同部位的脑组织水分含量的变化。

A random dorsal skin flap（3cm×9cm）was designed caudally or cranially in every rat of the group two and the group three respectively7days after the operation,and the viability of the flap was assessed byplanimeter.

SD大鼠30只，10只用于研究大鼠背部血管构筑；10只大鼠背部掀起一3cm×9cm的头端蒂随意皮瓣，10只大鼠背部掀起一3cm×9cm的尾端蒂的随意皮瓣，并原位缝合，术后7日，对皮瓣成活面积进行测量。

Methods: 50 normal Wistar male rats were randomly divided into five geoups: the sham operated group, injury model group, shenjindan capsule treat group, high dose and low dose group (duzhongyaotong pill treat, D and E)(n=10each). Autogenous nucleus pulposus was removed from the coccygeal vertebral and placed into the back of left L5~6 nerve root, The modle of the non-compressive nucleus pulposus protrusion was created by transplanting autogenous nucleus pulposus in the back of left L5~6 nerve roots. At tow weeks, the SP in dorsal root ganglion was determined by immunohistochemistry.

取健康Wistar大鼠50只，随机分为假手术组、模型组、对照组、高剂量组（D组、腰腿理痛散高剂量组）、低剂量组（E组、腰腿理痛散低剂量组），每组10只，将大鼠自身的尾椎髓核取出移植于左侧硬膜外腔L5、L6神经根背侧，造成非压迫性大鼠自体髓核移植硬膜外模型，通过免疫组化的方法测定大鼠2周时DRG中CGRP的变化。

Methods The rat models of heart failure were established by left anterior descending coronary artery deligation. After 6 w, 24 surviving rats were divided into sham,model and Ramipril groups at random,8 rats in each,and were treated with medicines by intragastric administration respectively. At 10 w, left ventricular enddiastolic pressure, left ventricular systolic pressure,+dp/dtmax anddp/ dtmaxof left ventricular pressure were measured.

结扎大鼠左冠状动脉前降支并饲养6 w的16只存活大鼠，随机分为模型组及雷米普利组，每组8只，另取8只大鼠为假手术组，连续灌胃给药4 w后测定大鼠血流动力学参数，ELISA方法检测血清及左心室非梗死区AngⅡ的含量，RTPCR 法测定左心室非梗死区心肌组织AT1R mRNA表达水平，Masson染色观察非梗死区心肌胶原的沉积。

Experimental study indicated: the flavone of rhizome drynaria can improve the cytoactive of marrow stroma cell of rats, promote its multiplicative division, and protect the marrow stroma cells' apotosis with lipidsion.The flavone of rhizome drynaria's serum can extently protect the osteoblast apoptosis of rats induced by TNF-α and the osteoblast senescence of rats induced by t-BHP.

实验研究表明：骨碎补总黄酮能够提高大鼠骨髓基质细胞的活性，促进其增殖分裂，并对脂质化大鼠骨髓基质细胞凋亡具有保护作用，而且骨碎补总黄酮含药血清对TNF-α诱导的大鼠成骨细胞凋亡和t-BHP诱导的大鼠成骨细胞衰老均具有一定的保护作用。

Methods Wistar rats of 6 months old were randomly divided into normal control groupand IR group. Each group had eight rats. Euglycemic insulin clamp technique was used to determine IR and glycose infusion rate was used to IR. Cardiocyte apoptosis was evaluated by TUNEL. Heart weight and body weight were measured to calculate HW/BW. Fine structure of heart and ultramicrostructure of cardiocyte were observed.

6月龄Wistar大鼠，分为正常对照组和IR组，正常血糖胰岛素钳夹技术测定各组大鼠IR，葡萄糖输注速率表示IR情况；测量大鼠心脏重量和体重并计算心脏重量与体重之比；原位末端酶标记法检测各组大鼠心肌细胞凋亡情况；观察心脏微细结构和心肌细胞超微结构；免疫组化检测心脏间质Ⅰ、Ⅲ型胶原水平。

Methods Both a rat model with habenular lesion and a hypnotic-treated rat model were established by passing a DC current or by infusing with zolpidem into the stomach and then the rats were divided randomly into habenular sham group,habenular lesion group,vehicle group and zolpidem group.The mRNA levels of adenosine and 5-HT-related genes were examined by RT-PCR in the PO/AH.

以30只雄性Wistar大鼠为实验动物，直流电损毁缰核或灌胃给予催眠药唑吡坦建立缰核损毁大鼠(n=15)和唑吡坦处理大鼠(n=15)两种动物模型，随后随机将大鼠分为缰核假手术组(n=7)、缰核损毁组(n=8)、生理盐水对照组(n=7)和唑吡坦处理组(n=8)。

It was found by means of protein fluorescence detection that the number of MAP-2 masculine neurone in the hippocamp of AD rat in model group and zinc reinforce post-AD group decreased distinctly. There was not distinct difference in the MAP-2 expression and the number of MAP-2 masculine neurone between normal group and zinc reinforce pro-AD group.

模型组和AD后补锌组大鼠海马区MAP-2蛋白表达与正常组大鼠相比显著下降，MAP-2蛋白荧光检测发现模型组和AD后补锌组大鼠海马区MAP-2阳性神经元数量明显减少，AD前补锌组大鼠海马区MAP-2蛋白表达和MAP-2阳性神经元数量与正常组相比差异无显著性。

Methods 25 Wistar rats were divided into control group (n = 5) and lidocaine group (n = 20),and rats in lidocaine group which received intratracheal injection of lidocaine were sacrificed at 2,24,48,and 72h (5 rats at each time point),then the samples of 1 / 3 lower parts of trachea were collected and observed by scanning electron microscope.

将25只Wistar大鼠随机分为正常对照组5只、利多卡因组20只，经大鼠气管穿刺注入利多卡因，并在2、24、48、72h4个时间点处死大鼠5只。取气管后1/3段的腹侧部，做扫描电镜观察大鼠气道黏膜表面结构。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。