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The arthropathy of AA can be efficiently prevented by oral administration of CⅡ.The standard of IL-2 in peripheral lymphoid tissue can be decreased by oral administration of CⅡ.

口服CⅡ可有效减轻佐剂型关节炎大鼠的关节病变。CⅡ能使AA大鼠淋巴器官中IL-2水平降低。

Results: The content of MDA, the expression of Caspase-3 and Bcl-xl were significantly increased in the ASCI control group as compared with the false-operation group.

80只SD大鼠,随机分为假手术组(n=16),ASCI盐水组(n-32)和ASCI治疗组(n-32),采用改良的Allen法,制成中度脊髓损伤大鼠模型;各组按术后3h、6h、24h、48h随机分为4个亚组;检测各亚组脊髓组织MDA的含量;以免疫组化检测各亚组脊髓组织Caspase-3表达和Bcl-xl基因表达。

Objective To establish obesity model of rats and study the effect of astragalus polysaccharides on insulin resistance in vivo.

目的 建立肥胖胰岛素抵抗大鼠模型,观察黄芪多糖对肥胖大鼠胰岛素抵抗的影响。

Objective: Augmenter of liver regeneration cloned from liver of weaning rat is a novel hepatic stimulator. It was reported that ALR mRNA expression was correlated with DNA synthesis of hepatocytes after partial hepatectomy, and could significantly promote the survial rate of intoxicated hepatitis rats.

目的:肝再生增强因子是从新生大鼠肝组织中克隆到的一种肝增殖刺激因子,其特异mRNA的表达与大鼠肝部分切除后肝细胞DNA合成密切相关,是一种特殊的促肝细胞分裂原,能特异地刺激肝细胞增殖,是肝再生的重要调控因子,能提高中毒性肝损伤动物的存活率,在肝损伤修复过程中发挥作用。

Objective It was reported that rALR (rat augmenter of liver regeneration) distribute in most of tissue and organ of rat, no-tissue specility; mRNA expression of rALR was identified variedly in different tissue, strongest in testis. But nowadays there was not some study on ALR in rat testis. Here to confirm the expression and localization of ALR in infancy,adult and old rat testis,comparing the change of ALR expression degree before per 70 PH and after per 70 PH. In order to evaluate expression principle of ALR in rat testis and know if ALR has tight connection with spermatogenesis and development of testis, to explore the relation between ALR and reproduction, to discover the possible expression character and functional methods of ALR.

目的:文献报道rALR分布于各组织器官,无组织特异性,但其mRNA在各组织的表达强度有差异,在睾丸组织表达最强,目前对大鼠睾丸ALR表达尚无深入的研究;本实验研究不同年龄段大鼠睾丸组织中肝再生增强因子的表达水平、表达部位、以及表达的方式,进而探讨睾丸ALR与睾丸发育、精子生成以及生殖活动的关系;同时探究睾丸组织ALR表达是否参与了肝脏的再生过程中,进一步了解睾丸ALR表达的特性及作用方式。

Animal experiment evaluated by functional and histological accessment 12 weeks after operation showed that there was autotomy in both groups.

动物实验结果显示,术后2个月,两组都出现残足情况,A组仅一只大鼠出现残足,而B组6只大鼠出现残足。

Methods: A rat model of endometriosis was established by autotransplant of endometria. Forty-eight endometriosis rats were randomly divided into 6 groups: untreated group, castrate group, mifepristone group (5 mg/kg) and low-(20 g/kg), medium-(40 g/kg) and high-dose (80 g/kg) CSG group.

采用自体移植法建立子宫内膜异位症大鼠模型,48只SD大鼠随机分为模型组、去势组、米非司酮(5mg/kg)组和低(20g/kg)、中(40g/kg)、高(80g/kg)剂量红藤颗粒剂组。

To determine whether a norm baric hypoxia cause's weight loss, 12 male 5-week old Wistar rats raised at an altitude of 2 260m were continuously exposed to a small hypoxic chamber with 10% O2, which is equivalent to 5 000m, for 20 days.

为了确定大鼠的体重是否在标准气压缺氧环境下会减轻,本实验将12只5周龄Wistar大鼠移高至海拔2 260 m并使之持续暴露于含10%O2的低氧小低压舱内(相当于在海拔5000m)20日。

Methods: Adopting pig bile culture medium, the inhibiting or killing effects on pig bladder worm in vitro were observed;by using the methods of xylene-induced ear inflammation in mice and granuloma swelling induced by cotton ball in rats, the effects of anti-inflammation were observed;by using the methods of body twisting and hot-plate in mice, the effects of analgesia were observed. Through the mice auricle microcycle, the rats thrombus law in vitro and bleeding time, clotting time determination to the mice, the function of invigorating the blood were observed;adopting the penicillin abdominal cavity injection method, the mice epilepsy model were prepared, and epilepsy mice brain organization MDA content and SOD, ATPase vigor be measured by using chemical colorimetry.

利用猪胆汁培养基观察该方在体外对猪囊尾蚴的抑制或杀灭作用;采用二甲苯所致小鼠耳肿胀法、大鼠棉球肉芽肿法以观察该方的抗炎作用;采用热板法和化学刺激扭体法观察该方的镇痛效果;通过小鼠耳廓微循环法、大鼠体外血栓法和对小鼠出、凝血时的测定观察该方的活血作用;采用青霉素腹腔注射法制备小鼠癫痫模型,并利用化学比色法检测癫痫小鼠脑组织中MDA的含量及SOD、ATPase的活力。

The results showed: the calcium, phosphate and ALP in blood in the osteoporosis rats induced by DXM were normal, the bone ash wight, bone dense and intestinal Ca2+ absorption were declined compared with these of the normal rats (p.05). Afterwards, the osteoporosis rats were treated with replenishing kideny herbs or calcium.

实验结果发现,地塞米松诱发的骨质疏松大鼠的血钙、磷、碱性磷酸酶及尿羟脯氨酸均在正常水平,骨密度、骨灰重下降,肠钙吸收减少,与正常大鼠比较有统计学差异(P.05),血中雌二醇、1,25-2-D3水平降低(P.01,P.05),PGE2升高(P.05),经过补肾中药或钙剂治疗后,血雌二醇、1,25-2-D3水平回升而PGE2回降,均接近正常水平。

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