大鼠

METHODS: Total 60 male Wistar rats were randomly divided into 6 groups, i.e., normal control group , DMN model group , large dose treatment group , middle dose treatment group, small dose treatment group and Dongbao gantai treatment group. The liver fibrotic model was induced by i.p. dimethylnitrosamine for 4wk. The relative herbs were given after model establishment in each treatment group and the administration lasted for 6 weeks. Decollate to get the blood and The serum hepatofibrosis indexes, hyaluronic acid, collagen IV, pro-collagen type III and laminin were measuredwith radioimmunoassay. SOD and MDA were measured with biochemistry method. The liver pathological changes were observed in each group of rats.

将60只雄性Wistar大鼠随即机分正常对照组、模型对照组、实验大剂量组(20ml/kg/d)、实验中剂量组(10ml/kg/d)、实验小剂量组(5ml/kg/d)及东宝肝泰药物对照组；腹腔注射二甲基亚硝胺制备大鼠肝纤维化模型，连续灌胃6周，断头取血，生化检测血清SOD、MDA；放射免疫法测定血清HA、LN、PCIII及IV-C；取肝左叶做HE染色和Mallory胶原染色，观察肝病理组织学改变。

The aim of this study was an evaluation of the influence of HiLoon aerobic performance and its physical effects. Elite swimmers、 collegestudents major in physical education and rats were exposed to simulatedaltitude in a decompression chamber and the followings were investigated:(1) erythropoiesis and erythropoietin,(2)maximal oxygen uptake,(3)body weight and body composition,(4)serum parameters of metabolism andhormone,(5)erythropoietin gene expression in kidney and vascularendothelium growth factor gene expression in heart.1 Effects of simulated "living high-training low" on aerobic performancein elite swimmersFourteen swimmers (10 men, 4 women) from Shandong swimming team weredivided into two groups: experimental group (living high-training low,n=8,6men,2women),living at a simulated altitude of 2,800m for 13 to 14 hours per day for 3weeks in a decompression chamber and training at sea level; control group(living low-training low, n=6,4 men,2 women),living and training at sea level.

为系统探讨高住低练对机体有氧能力的影响及其机制，本研究利用低压舱来模拟高原气压条件，分别以优秀游泳运动员、体育专业大学生和大鼠为实验对象，从以下几个方面来初步探讨模拟高住低练对机体的影响：(1)红细胞生成和EPO，(2)最大摄氧量和无氧阈，(3)体重和身体成分，(4)血清物质代谢指标及激素水平，(5)大鼠肾脏EPO和心脏VEGF基因表达。1 模拟高住低练对优秀游泳运动员有氧能力的影响 14名山东省游泳队运动员(男10人，女4人)，随机分为两组：实验组(模拟高住低练组，男6，女2)和对照组(低住低练组，男4，女2)。

Objective To observe the effects of berberine and taurine on PAI-1 mRNA expression in epiploon adipose tissue of high fat rats model of insulin resistance. and to study their effects on blood glucose, blood lipids, insulin levels, insulin sensitivity indexand body weight.

目的 观察黄连素、牛磺酸分别与联合应用对高脂饲料诱导的胰岛素抵抗大鼠脂肪组织 PAI-1 mRNA 表达的影响以及对大鼠空腹血糖、胰岛素、血脂、胰岛素敏感指数、大网膜脂肪细胞面积和体重的影响。

Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P＜0.001.

结果：构建了带有HSV-tk基因的反转录病毒载体GINaTK，应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317，成为产病毒细胞PA317TK，用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞，命名为C6TK细胞，对GCV和ACV的敏感性分别高于亲本450倍和10倍；成功地建立了大鼠脑胶质瘤模型，并证实转染细胞C6TK的成瘤效应未改变，存活期约为15天；而经ACV治疗后，含有C6TK细胞的肿瘤生长明显被抑制，大鼠生存期延长为57.8±8.07天，尤其是采用PA317TK细胞混和治疗组和原位治疗组，肿瘤基本消失，大鼠生存期显著延长，混和治疗组存活120天以上，原位治疗组存活至71.4±36.1天。t检验，P值均小于0.001。

Results: GINaTK retroviral vector containing HSV-tk was constructed and transduced into PA317 retrovirus packaging cell by lipofectin (named PA317TK). Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P ＜0.001.

结果：构建了带有HSV-tk基因的反转录病毒载体GINaTK，应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317，成为产病毒细胞PA317TK，用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞，命名为C6TK细胞，对GCV和ACV的敏感性分别高于亲本450倍和10倍；成功地建立了大鼠脑胶质瘤模型，并证实转染细胞C6TK的成瘤效应未改变，存活期约为15天；而经ACV治疗后，含有C6TK细胞的肿瘤生长明显被抑制，大鼠生存期延长为57.8±8.07天，尤其是采用PA317TK细胞混和治疗组和原位治疗组，肿瘤基本消失，大鼠生存期显著延长，混和治疗组存活120天以上，原位治疗组存活至71.4±36.1天。t检验，P值均小于0.001。

These Fos-immunopositive neurons were intensively distributed in different subnuclei of the vestibular nucleus complex (including medial, superior and spinal nuclei), nucleus of the solitary tract, locus coeruleus, medial and lateral parabrachial nucleus of the brainstem, paraventricular nucleus of the diencephalons and the amygdala of the limbic system;(3) The expression of Fos protein can be scarcely detected around forementioned regions in brains of the rats following complex two-axes rotation stimulation after the bilateral labyrinthectomy.

结果显示：(1)正常对照组和双侧迷路毁损组大鼠的脑内均未检测到Fos样免疫阳性产物；(2)两轴旋转刺激组的大鼠在给予复杂的围绕两轴旋转的加速度刺激后，在大鼠脑和脑干的多个核团内均可检测到Fos样免疫阳性神经元，其阳性产物主要表达于细胞核。

Conclusion］FSC with pedicle omentum transplanted after SCI enhance axon regeneration in the white matter,and can promote the recovery of neurological function.

结论］大鼠胚胎脊髓和大网膜移植后能增强损伤脊髓轴突再生并能促进大鼠后肢神经功能的恢复。

After a crush injury of sciatic nerve with digital electromagnetic pinchers in rats,the 2.5s nerve growth factorobserved under light and transmission electron microscopein different intervals of postoperation.

以坐骨神经损伤的大鼠为动物模型，采用形态学方法，研究了外源性短暂给予较大剂量神经生长因子后，对大鼠周围神经损伤修复的影响。

Methods Experimental ICH rats were established by injection of collagenase Ⅳ into caudate putamen.

通过大鼠尾状核注射胶原酶IV制作脑出血模型大鼠，分离培养胚胎神经干细胞移植入脑出血大鼠脑内。

Results: No significant difference were found befween A and B or C and D group before administration of TMP. In compare with the control group, TMP might increse HR and CO but decrease BP. The most marded changes of BP and CO appeared immediately agter the administration of the drug and refurned to the same leoel as premedication 5-7min later. The ± LVdp/dtmax and Time- tenson- index decreased firstly and increased later, reached the peak at 5min. In AMI group no obvious changes was found in HR. but BP decreased and CO increased.

注药前各监测指标在A、B两组及C、D两组间均无显著性差异，与A组相比，TMP可使健康大鼠出现明显的HR增快，BP下降，以及CO的增加，BP和CO改变的最大值均出现在给药即刻，5～7分钟内逐渐恢复至给药前水平，±LVdp/dtmax和时间一张力指数为先降后升，5分钟时达最高峰；在AMI大鼠组，TMP对HR无明显改变，但出现BP下降和CO增加，对±LV dp/dt max和TTI只有短暂的抑制，l～3分钟后恢复至用药前状态，无增强作用。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。