大鼠

Methods: One hundred and sixty SD rats were randomly divided into four groups （40 in each group）, and orally given the medicine with doses of 50, 25, or 12.5 g/kg of crude drug, or with bean oil of 0.5％CMC-Na daily for 26 weeks.

160只SD大鼠，随机分为高、中、低剂量组及空白对照组，雌雄各半，每日灌胃1次，连续给药26周，观察大鼠的一般状况、体重、摄食量和给药13、26周及停药后4周大鼠的血液学、血液生化学、。。。

Methods Intrathymic injection of 2.5×107 Wistar spleen cells in neonatal SD rats was performed followed by mixing lymphocyte culture with Wistar rat spleen cells (20Gy) at 4-6 weeks of age. The levels of IL-4 and IFN-γ were measured by ELISA on the third day.

将2.5×107个Wistar大鼠脾淋巴细胞注入新生SD大鼠胸腺内，4～6周龄时取其脾淋巴细胞与Wistar大鼠脾淋巴细胞(20Gy X射线照射)进行混合培养，于72h测定IL-4和γ干扰素的含量。

Methods: Randomly subgrouping the rat into normal group, model group, paishi granule up, and Yiqi Huoxue Paishi decoction group, except the normal and model group, rats in other groups were all intragastrically administrated with 1.25% Ethylene Alcohol plus 1% ammonia chloride, then to observe the Ca(superscript 2+), Mg(superscript 2+), P(superscript 3+), uric acid of the blood serum and urine, the ethanedioic acid in renal tissue.

随机将大鼠分为正常组、模型组、排石冲剂对照组和益气活血排石饮组，除正常对照组外，以1.25%乙二醇合1%氯化铵灌胃制作大鼠草酸钙肾结石模型，观察各组大鼠血清Ca(上标 2+)、Mg(上标 2+)、P(上标 3+)、尿酸及肾组织草酸以及尿Ca(上标 2+)、Mg(上标 2+)、P(上标 3+)、与尿尿酸的含量。

During the period of making model, the rats in group of ZGF low dosage and group of ZGF high dosage were fed with ZGF at different dosages. The experiment was ended after eight weeks, and the autopsied liver tissues from each rat were checked for PCNA and AFP by immunohistochemistry method.

造模期间，将不同浓度正肝方水剂(0.6g/ml， 0.3g/ml)分别灌喂正肝方预防组大鼠；用无菌蒸馏水灌喂模型组大鼠。8周后处死大鼠，肝组织取材。

The purpose of the present study was to investigate the role of central arginine vasopressin in corticotropin releasing hormone-induced fever in the rat. Guide cannulae were inserted into the third ventricle and placed over the ventral septal area. The content of arginine vasopressin in the VSA of the brain was determined by radioimmunoassay. Colon temperature was monitored in lightly restrained rats by insertion of a catheter-mounted thermistor probe 5 cm in the rectum.

实验对大鼠进行第三脑室和脑腹中隔区插管，用数字体温计测量大鼠的结肠温度，用放射免疫分析法测定脑中隔区精氨酸加压素(arginine vasopressin， AVP)含量，观察脑中隔区AVP在大鼠促肾上腺皮质激素释放激素(corticotrophin releasing hormone， CRH)性发热机制中的作用。

Methods Ten mature Wistar rats were divided into normal control group (5 rats) and adenovirus (E1, E3-Deleted and carried math1 and enhanced green fluorescent protein report gene, Ad-Math1-EGFP) scala vestibuli transfer group (5 rats). Right ears of the Ad-Math1-EGFP transfering group rats were deliveried 5μl Ad-Math1-EGFP (physical tite 2.1×10^11v.p./ml) into cochleas through the way of drilling scala vestibuli of cochlear basal turn. As a control, the normal group received nothing to inner ear. In order to estimate functional condition of vestibule and cochlea, the click-evoked potentials on the surface of the cervical dura mater, auditory brain stem response and swimming time were recorded in all rats at 7 days after treatment, and then histologic and morphologic observation were carried out after animals were sacrificed. Results All animals' morphologic observation showed that inner ear hair cells were normal after transfer.

将10只成年Wistar大鼠分为正常对照组和缺失E1、E3基因片段且构建有Math1基因和绿色荧光蛋白报告基因的复制缺陷型腺病毒(adenovirus-Math1-enhanced green fluorescence protein， Ad-Math1-EGFP)前庭阶导入组，每组5只，实验组大鼠在右耳通过耳蜗底回前庭阶打孔的方法导入物理滴度为2.1×10^11v.p/ml的上述腺病毒5μl，对照组大鼠不做任何处理。7天后对动物进行颈髓硬膜外短声诱发电位(click-evoked potentials on the surface of the cervical dura mater， CDM-CEP)、听性脑干反应阈值检测和游泳试验，评价前庭和耳蜗功能，然后将动物处死进行组织形态学观察。

It was shown that: 1 MMP2 activity reached the highest level during proestrus, and was lowest during diestrus; 2 MM2 acti vity was increased from the first day of pregnancy, peaked on day 4 of pregnancy; but decreased significantly at day 7 of the pregnancy. 3 MMP2 activity was obviously higher on day 1 of pseudopregnancy than on the other days, and its activity decreased gradually, reached the very low level at day 4 of pseudopregnancy and maintained the low level until day 7; 4 MMP2 activity was decreased significantly 24 hours after injection of 17βestradiol ( 01 mg) at metaestrus, while MMP2 activity had no change after progesterone (04 mg) injection.

明胶酶A活性变化如下：1在大鼠的动情前期明胶酶A的活性最高，间情期最低；2在妊娠早期(1～7天)的卵巢中，明胶酶A的活性在妊娠第4天达到最高，第1～3天及第5、6天其活性也较高，但第7天则明显下降；3在假孕(1～7天)大鼠的卵巢中，明胶酶A的活性在假孕第1天最高，第2、3天略下降，从第4天到第7天，其活性都处于较低水平；4动情后期大鼠皮下注射雌二醇(0.1mg/只)24小时后，明胶酶A活性明显上升。

Methods The rats were given with Huanggui enema by enemata and the paw swelling and pain threshold of rats were observed; the rabbits were given with Huanggui enema by enemata and the fever reaction of rabbits was observed.

用黄桂灌肠剂给大鼠灌肠，观察其对角叉菜胶所致大鼠足跖肿胀、大鼠痛阈值的影响。用黄桂灌肠剂给家兔灌肠，观察其对家兔发热的影响。

It is inferred that Gukang can increase mineral contents and bone density in eunuchized male mice,promote the reconstruction of bone and improve the inner structure of bone,so as to improve the mechanical function of bone and prevent from mice fracture.

骨康能提高去睾大鼠骨的骨矿含量及骨密度，促进去睾大鼠骨结构的再建，改善骨的内部构造，从而提高大鼠骨的机械性能，避免骨折的发生。

Methods MID was established by inverse injection of thrombus into external carotid artery to observe the pathologic changes in hippoeampus and cortex region of brain and the ability of spatial learning and memory with Morris Water Maze.

颈外动脉逆行插管注入血栓建立MID大鼠模型，观察大鼠大脑皮质及海马的病理形态变化，同时应用Morris水迷宫检测MID大鼠的空间学习记忆的变化。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。