大鼠

Culture and immunohistochemistry of Schwann cells Schwann cells that are derived from sciatic nerve of Sprague-Dauley rats are successfully isolated, cultured. The Schwann cells are transplanted into the hemisected adult rat spinal cord after purification and condense and enclosed by L I -antibody. Eight weeks later, the experiments of the S- 100 immunohistochemistry indicate that Schwann cells are still alive and array orderly relatively.

本实验的主要研究内容及结果如下： 1、雪旺细胞的培养及免疫组化染色利用新生S-D大鼠的坐骨神经成功地分离、培养获得了纯度较高的雪旺细胞，并将雪旺细胞移植进入大鼠脊髓损伤创面，饲养8周后经过S-100免疫组化ABC染色发现雪旺细胞在大鼠体内成活良好并成簇排列。

The results showed as:(1)Gegen decreases fasting insulin and insulin resistance index and increases insulin sensitive index of rats of insulin resistance induced by dexamethasone significantly;(2)Gegen can improve glucose tolerance of rats of insulin resistance but not to alloxan indued diabetes mice;(3)Gegen increases glucose intake of 3T3-L1 adipocytes and improves the insulin resistance of 3T3-L1 adipocytes induced by dexamethasone significantly;(4)Gegen can activate peroxisome proliferator- activated receptor gamma, make it bind to PPAR response elements;(5)Gegen can up-regulate mRNA expression of PPAR v ,aP2 and adiponectin.

结果表明：①葛根显著降低地塞米松诱导的胰岛素抵抗大鼠血清胰岛素水平和胰岛素抵抗指数，升高胰岛素敏感指数，改善大鼠的胰岛素抵抗；②葛根显著改善胰岛素抵抗大鼠的糖耐量，但对四氧嘧啶糖尿病小鼠的糖耐量没有明显的作用；③葛根显著增加胰岛素抵抗3T3-L1脂肪细胞对葡萄糖的摄取，改善地塞米松诱导的3T3-L1脂肪细胞的胰岛素抵抗；④葛根能促进HepG2肝癌细胞过氧化物酶体增殖物激活受体γ与靶基因的特异DNA序列结合，对PPARγ及其下游基因的转录具有激活作用；⑤葛根能够促进PPARγ及其下游基因脂肪酸结合蛋白(aP2)、脂联素mRNA的表达。

Results: Xuesetong protected the acute myocardial injury in rats by ligaturing the left descending coronary artery and reduced myocardial infarct range. Xuesetong decreased the CPK, LDH and the production of MDA, improved the activity of SOD in myocardium.

结果：血塞通可对抗结扎大鼠冠状动脉左前降支所致的大鼠急性心肌损伤，使心肌缺血大鼠的CPK及LDH降低，并可增加心肌组织SOD的活性，减少MDA生成且能显著缩小心肌梗死的范围。

Methods Fifty F-344 rats were divided into four groups, GM only、PAA+GM、PAA only and saline control. ABR thresholds at different frequencies were measured at different times and hair cell losses were numerated. Two-dimensional diffusion assays in the culture medium were performed to evaluate the effect of PAA on the antimicrobial activity.

选用健康F-344大鼠50只，随机分4组：Ⅰ为GM、Ⅱ为PAA+GM、Ⅲ为PAA、Ⅳ为生理盐水对照组；通过观测4组大鼠不同时期、不同频率听性脑干反应（auditory brainstem respons，ABR）阈值的改变；计数耳蜗毛细胞死亡率，以观察PAA对F-344大鼠GM耳蜗毒性的拮抗作用；用双向扩散血清培养基检测法观察PAA 对 GM 抗菌活性的影响。

The mean arterial pressurewere measured in each rat.the stereological parameters such as the myocardial surface density、the myocardial volume density、the myocardial specific surface and the mean free path etc.were measured and numerated.

建立腹主动脉缩窄性高血压大鼠模型，测定大鼠平均动脉压、大鼠心肌的表面积密度、体积密度、比表面及平均自由程等各项体视学参数并计算。

Study of peripheral nerve lesions and the effects of the recipe of supplementing Qi and activating blood circulation on it in streptozotocin-induced diabetic rats: It was showed that the weight, MNCV and SNCV were significantly reduced, H reflex were significantly prolongated, the level of blood sugar were significantly increased in diabetic rats compared to normal rats at 1 week, 3 week and 5 week after STZ injection (p.05), some changes of the sciatic nerve structure were occurred after 5 weeks of the diabetic progress.

STZ大鼠周围神经功能变化及益气活血方对其影响的实验研究：STZ诱发的糖尿病大鼠，成模1W后体重减轻、血糖升高、SNCV、MNCV减慢、SNCVamp减小、SNCVlat及H反射延迟，以上各项指标与对照组相比差异显著（P均。05）：成模5W后上述改变更为明显。成模5W后大鼠坐骨神经纤维出现髓鞘变性、融合、部分神经纤维变性等形态改变。

The number of BrdU labeling in the nucleus was remarked increasing onbFGF-treated pancreatitis rats than pancreatitis rats without bFGF treatment. And there was a significant difference.(P.01)ConclusionThe result of the study has demonstrated the effective therapeutic effects on acute edematous pancreatitis rats, which induces proliferation of immature acinar cells, facilitates the repair of damaged pancreatic tissues and reconstitutes tissue integrity.

兔疫组化结果显示bFGF治疗组大鼠具有BrdU标记的细胞核胰腺细胞数明显多于非治疗对照组大鼠，两者在统计学上具有显著性差异Oeq.of人结论实验结果表明bFGF对急性水肿性胰腺炎大鼠具有明显的治疗作用，它的治疗作用具体表现在诱导非成熟的胰腺细胞分化增殖，促进受损胰腺组织修复，重建组织完整性。

There were no death rate increase in CRF experimental models established by resecting off 5/6 kidney from male sprague-dawley rats in one stage .

1、我们通过改良背部切口，行同期大鼠一侧肾切除对侧2/3肾切除建立CRF大鼠模型，大鼠死亡率无明显增加，能保证实验完成的要求。

The results indicate that the free radical activity in kidney tissue increases significantly after exercise. As for dynamic changes of LPO, SOD and GSH-Px index based on four time tests, endurance exercise may increase the antioxidative enzyme activity in Kidney tissue, and as a result, it scavenges much more free radical resulting from exercise and enhances the ability for anti-free radical.

大鼠肾脏组织在运动结束后即刻自由基作用明显加强，从其在运动结束后4次测得肾脏组织LPO、SOD、GSH-Px和尿TP排出率指标所形成的动态变化看，耐久性运动可以提高大鼠肾脏组织抗氧化酶活性，从而清除运动时产生的大量自由基，提高大鼠肾脏组织对抗自由基的能力。

Methods SD rats and Wistar rats were used in the experiment. P.c DNA from rat lung tissue and BALF was amplified by PCR.

实验组患卡氏肺孢子虫肺炎的SD大鼠和Wistar大鼠各 2 8只，采用PCR法扩增大鼠肺组织DNA和支气管肺泡灌洗液DNA ，用PCR ELISA检测其扩增产物。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。