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大鼠

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When there is 5 minutes after ischemia, acupuncture group is acupunctured and medicine group is injected GM|.

实验选取Wistar大鼠178只为受试对象,通过线栓法造成大鼠左侧大脑中动脉闭塞,建立大鼠急性局灶性脑缺血模型,缺血后5分钟给予针刺组针刺,药物组给予腹腔注射施捷因注射液。

When there is 5 minutes after ischemia, acupuncture group is acupunctured and medicine group is injected GM|. The content of serum calcium, magnesium of models, acupuncture and medicine group have been measured in lh,2h,3h,5h,7h,12h,24h following cerebral ischemia and those of normal group have measured directly.

实验选取Wistar大鼠178只为受试对象,通过线栓法造成大鼠左侧大脑中动脉闭塞,建立大鼠急性局灶性脑缺血模型,缺血后5分钟给予针刺组针刺,药物组给予腹腔注射施捷因注射液。

Methods: One hundred and sixty SD rats were randomly divided into four groups (40 in each group), and orally given the medicine with doses of 50, 25, or 12.5 g/kg of crude drug, or with bean oil of 0.5%CMC-Na daily for 26 weeks.The physical status (body weight,growth rate and appetite), hematological parameters, blood biochemistry, blood cotting, organ index, and tissue pathological changes of rats were checked in the 13th week,and 26th week of the administration and in four weeks after the stop of the administration.

160只SD大鼠,随机分为高、中、低剂量组及空白对照组,雌雄各半,每日灌胃1次,连续给药26周,观察大鼠的一般状况、体重、摄食量和给药13、26周及停药后4周大鼠的血液学、血液生化学、凝血功能、脏器系数、解剖学及病理组织学改变的情况。

The physical status (body weight,growth rate and appetite), hematological parameters, blood biochemistry, blood cotting, organ index, and tissue pathological changes of rats were checked in the 13th week,and 26th week of the administration and in four weeks after the stop of the administration.

160只SD大鼠,随机分为高、中、低剂量组及空白对照组,雌雄各半,每日灌胃1次,连续给药26周,观察大鼠的一般状况、体重、摄食量和给药13、26周及停药后4周大鼠的血液学、血液生化学、凝血功能、脏器系数、解剖学及病理组织学改变的情况。

The results showed that:(1) In COPD group the alveolar walls were broken. The average area of alveolars was 2.46 times as big as the control group, and the average diameter was 1.42 times as compared with the control group. As the diameter of bronchioles was less than 100μm and 100~200μm respectively, the average area of smooth muscle in bronchioles increased to 3.06-fold and 2.13-fold respectively. By electron microscopic, the proportion of Clara cells of respiratory bronchioles in COPD group decreased, the Clara cells were denaturalized, the proportion of Clara cells containing low density secretory granules increased. The airway resistance was 1.84 times while the dynamic compliance was only 70.45% comparing with the control group.

结果表明:(1)光镜下,COPD组大鼠与正常对照组比较,肺泡壁破坏、肺泡腔扩大,平均肺泡面积和平均肺泡直径分别为正常对照组的2.46倍和1.42倍,在直径<100μm及直径100~200μm的小支气管,COPD组大鼠支气管平滑肌面积分别为正常对照组的3.06倍和2.13倍;电镜下可见,在呼吸性细支气管,Clara细胞占上皮细胞的比例下降,细胞变性,含低电子密度颗粒的Clara细胞比例增加;呼吸功能测定可见COPD组大鼠呼气相气道阻力是正常对照组的1.84倍、动态呼吸系统总顺应性为正常对照组的70.45%。

Methods: On the basis of developing rats model of BRG by using method of bile-regurgitational liquid-filling, the rats were douched with different herbs, or normal saline respectively in different groups for 4 weeks, the effect of JJSNS to pathological histology was observed, and the gastrin in serum, molitin in plasma and the mucosal 6-keto-PGFu were measured by radioimmunoassay, and compared with Xiangshayangwei pill.

采用返流液灌胃方法复制大鼠胆汁返流性胃炎模型,以大鼠胃粘膜病理组织学、血清胃泌素含量、血浆胃动素含量及胃粘膜6-酮-前列腺素_(1α)6-keto-PGF_(1α含量为观察指标,观察加减四逆散对胆汁返流性胃炎大鼠的治疗作用,并与中成药香砂养胃丸治疗组进行对比观察。

Methods: The levels of serum sex hormones in diabetic rats, normal rats and streptozotocin rats were detected by radioimmunoassay and their sexual glands were collected and weighed and the histomorphology of testes, prostate glands and epididymides was observed under a microscope.

用放射免疫法检测糖尿病大鼠,正常大鼠和STZ大鼠血清性激素含量,同时称取性腺重量,镜检睾丸、前列腺及附睾的组织形态学改变。

objectiveto study the effects of glycyrrhizin on intestinal absorption of paeoniflorin by the everted rat gut sac model.methodsused a simple rp-hplc method for the determination of paeoniflorin in the krebs fluid.

目的研究甘草酸对芍药苷大鼠肠吸收的影响。方法采用大鼠肠外翻模型研究芍药苷的肠吸收,并用反相高效液相色谱法测定大鼠肠黏膜内外两侧的芍药苷浓度。

Methods The contrast group was the normal adult rats and the ovariectomized rats injected by plant oil,the experiment group was the rats extirpated ovaries who were injected VE in its′ abdomen antrum:5 mg/kg,15 mg/kg,60 mg/kg VE was injected to the experiment group for continuous 9 weeks.

正常成年大鼠和注射植物油的去卵巢大鼠为对照组;腹腔注射VE的去卵巢大鼠为实验组,分别为:5 mg/kg、15 mg/kg(中剂量组、60 mg/kg,连续注射9周。

Objective: This experimental study is based on earlier animal and clinical experiment, drawn thinkings of combinations with two methods and pursued a synthesis method of high-efficiency, cheap and convenient calcium and exercise treatment. We adopt taking natural-clam's casing powder and preconcereted-intensity excises to intervene shape of osteoporosis in gelded rats, than we compare the indexes such as the biomechanics indexes of the right femur and the third lumbar vertebrae, the weight and uterus quotiety of rats before the experiment to these indexes after the experiment. We hope to find better and suitable way to prevent and treat the postmenopausal osteoporosis and offer reference for clinic.

目的 本实验的主要目的是在前期动物实验的基础上,引入两种方式联合干预的思维,立足于探求既高效又经济、方便的补钙剂加上与运动疗法相联合的方式,对切除雌性大鼠卵巢而复制成的骨质疏松症模型进行服用天然贝类蛤蜊壳粉,同时施以预定强度的运动刺激的方法,再通过试验前后大鼠体重、子宫指数的变化及大鼠股骨和椎体生物力学指标的对比变化进行分析,以期探索出防治绝经后骨质疏松症的更好的、更适宜于大众的手段;并为天然钙剂和运动联合作用防治PMOP提供参考。

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