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After 2, 4, 6, and 8 weeks of feeding, 2% fluorescein sodium was dropped into rat conjunctival saccus and breakup time of tear film was observed by cobalt blue light scanning under slit lamp microscope. Then the rats were executed after anesthesia.

分别饲养2,4,6,8周后,用2%荧光素钠滴入大鼠结膜囊内,瞬目后在裂隙灯显微镜下用钴蓝光扫描照射观测大鼠的泪膜破裂时间,然后麻醉下颈椎脱臼法处死大鼠

The amount of middle molecule substance in plasma of normal rats and experimental schizophreniac rats changed without obvious rhythm, but during spring and summer.

正常大鼠、精神分裂症模型大鼠血浆中分子总量一年四季无明显节律变化,SP模型大鼠中分子组分有春夏季病理组分增高的趋势。

Methods 24 SD rats were randomly divided into normal group,model group,aminophylline treatment group(35 mg/kg), 8 rats in each group. The bronchial asthma model in rats was built by egg protein sensibilization and longterm inhalation provocation. The rats in treatment group were lavaged each day from the first time of provocation.After 4week treatment, the rats were killed to obtain lung tissue for staining with HE.

24只SD大鼠随机分为正常组、模型组、治疗组,每组8只,除正常组外以卵蛋白致敏并吸入激发法制备大鼠哮喘模型,治疗组、模型组从第1次哮喘激发开始(造模第3周)分别给予氨茶碱、0.9%氯化钠溶液灌胃给药,1次/d,用药4周处死大鼠,取肺组织HE染色,用彩色图像分析仪测量支气管壁面积、支气管平滑肌面积,用ELISA双抗体夹心法测定肺组织MMP9和TIMP1含量。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞(non-hematopoietic adult stem cells,NASC)体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同,在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓,用Ficoll-hypague分离液分离出单个核细胞后,种植于含10%胎牛血清的DMEM/LG培养液内。收获的NASC传代培养,用流式细胞仪检测细胞的免疫表型。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞(non-hematopoietic adult stem cells,NASC)体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同,在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓,用Ficoll-hypague分离液分离出单个核细胞后,种植于含10%胎牛血清的DMEM/LG培养液内。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum. The acquired NASCs were subcultured for passage.

为了比较大鼠胎血和骨髓中非造血成体干细胞(non-hematopoietic adult stem cells,NASC)体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同,在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓,用Ficoll-hypague分离液分离出单个核细胞后,种植于含10%胎牛血清的DMEM/LG培养液内。

Methods Eighty Class Ⅱ isogeneic F344 rats, weighing 250-350 g, were randomly divided into 2 groups(n=40). The control group adopted the Strome model. The allograft used for the experimental group preserved ascending pharyngeal artery, which formed a complex allograft together with tongue base, larynx and pharyngolarynx. End-to-end anastomosis was performed between both allograft common carotid arteries and anterior neck veins. The patency rate and survival rate of the allograft of the 2 groups were compared.

80只Ⅱ级近交系F344大白鼠,体质量250~350 g,随机分为2组,每组40只,共20对对照组采用Strome喉移植模型,实验组在切取供体喉时保留咽升动脉,将舌根、喉、喉咽作为复合移植体,供体大鼠双侧颈蓉动脉分别与受体大鼠颈总动脉、颈前静脉进行端端吻合重建移植喉血液循环,比较两组术后动静脉通畅率及移植喉存活率。

The subacutely aging rats were made by injecting Dgal into the rats' abdominal cavity continuosly. The learning and memory ability of rats in each group were detected with Morris water maze. The monoamine transmitter contents in hypothalamus and hippocampus were detected with HPLCECD.

D半乳糖连续腹腔注射制作亚急性衰老的大鼠模型,采用Morris水迷宫检测各组大鼠的空间学习记忆能力及采用高效液相色谱电化学法检测各组大鼠下丘脑、海马NE,DA,5HT的含量。

With computer image analysis system, the expressions of NF-κB and ICAM-1 and subepithelial collagen deposition were checked.

将40只大鼠随机分为对照组、哮喘组(以卵蛋白激发的大鼠哮喘模型)、布地耐德早期治疗组、布地耐德晚期治疗组,应用免疫组化技术结合计算机病理图像分析系统测定大鼠气道上皮胶原的沉积和气道上皮细胞NF-κB、ICAM-1的相对含量。

Middle and less dose suppling of Ginkgo biloba cause low expression of c-fos in each area of hypothalamus but for dentate gyrus.

大鼠给予银杏叶片后,炎性致痛大鼠海马区及下丘脑区的c-fos揭示了银杏叶片在疼痛大鼠的中枢神经系统对c-fos有一定的影响作用。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

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There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。