大鼠

We introduced improved primary mixed glial culture and different-attachment method to isolate and purify the OPCs, the cells were proliferated in serum-free medium, flow cytometry and immunohischemistry methods were employed to estimate the purity of cultured OPCs. Their abilities of differentiation and expression of trophic factors were identified by RT-PCR and immunostaining. Several methods including TUNEL and MTT were adopted to estimate the protective effects of conditioned culture medium from oligodendrocyte lineage cells on the primary cultured cerebellar granule neurons. Intravitreal transplant of OPCs, combined with retrograde fluorescent labeling the superior colliculus and intraorbital optic nerve transection, were used to investigate the protective effects of OPCs on the axotomized RGCs in vivo. Intravitreal transplantof OPCs or NSCs on the newborn rats, and retinal transplant of OPCs on the young rats were performed, to observe the myelin formation in the retina at different stages after cellular transplantation. Optic nerve transection was carried out on some rats with myelinated retinae, to study the influence of myelination on the injuried RGCs.

为此，本研究采用改良的胶质细胞混合培养与差速贴壁方法获得大鼠OPCs，使用无血清培养基进行扩增、培养，用免疫组织化学和流式细胞技术对培养细胞的纯度进行鉴定，对少突胶质系细胞表达部分营养因子的情况进行检测；采用TUNEL、MTT等方法对少突胶质系细胞条件培养基对原代培养小脑颗粒神经元的保护作用进行检测；将OPCs移植入成年SD大鼠玻璃体内，利用上丘逆行荧光标记技术，观察眼内移植的OPCs对眶内视神经切断时的视网膜神经节细胞的保护作用及其持续时间；将OPCs或NSCs移植入新生和幼年SD大鼠玻璃体或视网膜内，观察不同时期视网膜内髓鞘形成与分布特点，分析髓鞘的超微结构，并观察眼内髓鞘形成对损伤神经节细胞的保护作用。

Methods All of rats were randomly divided into seven groups and were intratracheally infused with bleomycin to build the model.

大鼠麻醉后，经气管软骨环间隙向心端穿刺，气管内注入博莱霉素A5(5 mg/kg)建立大鼠肺纤维化模型，假手术组大鼠在同样麻醉条件下气管内注入等量生理盐水。

Sugar water choosed degree have no significant change. It means that Iridoids composition of Valeriana jatamansi Jones has central inhibitory action.2.2 Effection of Iridoids composition of Valeriana jatamansi Jones on weight and food intake of IBS rats induced by chronic stressAnimals were randomly divided into 7 groups:normal group,model group, OSD group,FXT group and Iridoids composition of Valeriana jatamansi Jones groups in quantiy of 24.92mg/kg,12.46mg/kg,6.23mg/kg.

蜘蛛香环烯醚萜可以减缓正常小鼠的胃肠运动和利血平化小鼠的胃肠功能亢进，降低慢性应激所致IBS模型大鼠的内脏敏感性，显著减少了IBS模型大鼠的排便数量，从而改善腹泻型IBS的临床症状。2蜘蛛香环烯醚萜对神经系统功能的影响目的：探讨蜘蛛香环烯醚萜对精神活动的影响2.1蜘蛛香环烯醚萜对慢性应激所致IBS大鼠精神活动的影响方法：动物随机分为对照组、模型组、昂丹司琼组、氟西汀组、蜘蛛香环烯醚萜24.92mg/kg、12.46mg/kg、6.23mg/kg组，除对照组外，其余各组进行慢性应激实验。

It means that Iridoids composition of Valeriana jatamansi Jones has central inhibitory action.2.2 Effection of Iridoids composition of Valeriana jatamansi Jones on weight and food intake of IBS rats induced by chronic stressAnimals were randomly divided into 7 groups:normal group,model group, OSD group,FXT group and Iridoids composition of Valeriana jatamansi Jones groups in quantiy of 24.92mg/kg,12.46mg/kg,6.23mg/kg.

蜘蛛香环烯醚萜可以减缓正常小鼠的胃肠运动和利血平化小鼠的胃肠功能亢进，降低慢性应激所致IBS模型大鼠的内脏敏感性，显著减少了IBS模型大鼠的排便数量，从而改善腹泻型IBS的临床症状。2蜘蛛香环烯醚萜对神经系统功能的影响目的：探讨蜘蛛香环烯醚萜对精神活动的影响2.1蜘蛛香环烯醚萜对慢性应激所致IBS大鼠精神活动的影响方法：动物随机分为对照组、模型组、昂丹司琼组、氟西汀组、蜘蛛香环烯醚萜24.92mg/kg、12.46mg/kg、6.23mg/kg组，除对照组外，其余各组进行慢性应激实验。

Then the index of ulcer was measured and the ulcer inhibitory rate was measured.Results: The weight of ear of mice treated with XSG was reduced compared with that of normal group,and the joint swelling of rat was less than that of normal group. The latence of sole-ache caused by hot-plate and bellyache caused by acetic were both delayed and the times of skewing body within 15 minute were reduced compared with that of the normal group.

结果：香砂六君颗粒对小鼠二甲苯性耳廓肿有明显对抗作用；对大鼠蛋清性关节肿有一定对抗作用；对热板和醋酸所致小鼠疼痛香砂六君颗粒能明显延长其舔足潜伏期和减少扭体次数；对大鼠利血平型及乙酸型胃溃疡香砂六君颗粒组的溃疡指数从模型组的46.26±1.64mm2和23.02±2.05mm2减少为34.75±0.82mm2和13.84±1.2mm2；对大鼠利血平型及乙酸型胃溃疡香砂六君颗粒的溃疡抑制率分别为51.43%和39.87%。

After HRP application to the rostral area (n=10), quite a number of labelled cells were distributed at the ventrolateral part of the nucleus of trapezoid body, the lateroventral periolivary nucleus and the retrotrapezoid nucleus. After HRP application to the caudal area (n=10), HRP labelled cells were mainly located at the lateral reticular nucleus and the ventral part of at the lateral paragigantocellular nucleus LPGi. After HRP application to the intermediate area (n=6), only 8HRP labelled cells were observed at the in 6rats. After HRP application to the control area (n=4), only 6HRP labelled cells were found at the rostral ventrolateral reticular nucleus.

大鼠延髓腹外侧表面的头端化学感受区表面敷贴辣根过氧化物酶后，在延髓斜方体核、外周橄榄腹外侧核和斜方体后核均有HRP标记细胞存在；于尾端化学感受区表面敷贴HRP后，在延髓的巨细胞旁外侧核、外侧网状核和舌下神经核有HRP标记细胞存在；于中间区表面敷贴HRP后，在6只大鼠的延髓内部区域仅观察到8个HRP标记细胞；于对照区敷贴HRP后，在4只大鼠的延髓内部区域仅观察到6个HRP标记细胞，散在地分布在头端腹外侧网状核。

The effect of F 1C on joint swelling and the immune respones of AA rats were evaluated by lymphocytotic reaction, flow cytometry, and ELISA. Results F 1C by ig administration to AA rats decreased the primary joint swelling of AA rats, stronger than TW and weaker than CsA, promoted the decreased splenocyte proliferation induced by Con A, and inhibited the augmented thymocyte proliferation induced by Con A in AA rats. The evaluation in vitro showed the F 1C inhibited the augmented thymocyte proliferation from AA rats and promoted the deficient IgG produced by splenocyte from AA rats.

结果 ig给予F1C(30mg／kg)对AA大鼠原发性足肿胀具有明显的治疗作用，该作用较TW强，较CsA弱；F-1C对AA大鼠低下的脾细胞增殖反应具有改善作用；对亢进的胸腺细胞增殖反应具有抑制作用。F-1C体外对AA大鼠低下的脾细胞产生IgG水平具有明显的促进作用，并能抑制亢进的胸腺细胞增殖反应。

The expression levels of VEGF and FLT1,FLK1 mRNA in equal area of penumbra were measured with situ hybridization in different times after MACO (1 h,3 h,6 h,12 h,24 h). The difference was compared between DMCI group and no diabetes cerebral infarction group.

建立DMCI大鼠模型，在脑缺血后1 h、3 h、6 h、12 h、24 h，采用原位杂交方法测定大鼠大脑缺血半暗带等值区VEGF、FLT1、FLK1 mRNA的表达水平；并与无糖尿病的脑梗死大鼠比较。

Vascular endotheliocyte fluorescence staining and leukocyte fluorescence tracing method were applied, in combination with microscopic video system and computer imaging analyzing system, to observe in dynamic and quantitatively the influences of acupuncture on pia matral encephali microvascular morphology, density and blood flowing velocity at 3 hours, 6 hours and 24 hours after cerebral occlusion of meddle cerebral artery.morphology, density and blood flowing state at various time divisions observed directly microscopically and vascular endotheliocyte morphology and the state of brain tissue which near the microvascular after infusion with velocity in infarcted area at various time divisions measured in quantity in each group.microvascular endotheliocytes were colored worse and more fluorescent exudation appeared.

采用血管内皮细胞荧光染色及白细胞荧光示踪法，结合显微录像系统和计算机图像分析系统，动态定量地观察了针刺对大脑中动脉阻塞后3，6，24h软脑膜微血管形态、密度、血流速度的影响。主要观察指标：①从显微镜下直接观察各组大鼠不同时段软脑膜微血管的形态、密度、血流状况，以及注入荧光剂后血管内皮细胞形态和周围脑组织状况。②定量测量各组大鼠不同时段梗死区微血管密度、血流速度。结果：120只大鼠均进入结果分析。

Methods: Forty mice were divided into four groups randomly:normal group,model group,ligation group and treatment group.The model was made by the method of partly ligating the recta outside the body.The level of endotoxin in blood plasma was measured by dynamic nephelometric method and the content of TNF-a in blood serum was detected by the method of radioimmunity.(2)After the mice were killed,their left lungs were taken out for bronchi-alveoli fluid, then the content of MDA in BALF was tested by TBA method.(3)The vitality of PAM in BALF was countied on the counting set of erythrocyte,and the blue-dyed cellswere counted, then the percentage of PMN was worked out.(4)The pathological features of the mice\'s lung tissue were observed with routine and ultramicro pathological mechanism.

①将40只大鼠随机等分为正常组、模型组、解扎组、治疗组，以体外直肠不全结扎法造模，以动态浊度法检测各组大鼠血浆内毒素水平，放射免疫分析法检测各组血清TNF-α含量；②大鼠处死后，取左肺进行支气管肺泡灌洗，以TBA法测定支气管肺泡灌洗液中丙二醛含量、白蛋白含量及肺表面活性物质水平；③在红细胞计数盘上作BALF中肺泡巨噬细胞活力计数，计数兰染的细胞数，求出死亡百分率；在光镜下计数BALF沉渣200个细胞，计算中性粒细胞所占百分比。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。