大鼠

Itis a great deal of assistment for treatment and prognosis of patients, if we candiagnose hepatic fibrosis in forepart. The aim of this experiment evaluate the dignosisvalue of ultrasound elastic imaging on early hepatic fibrosis.we will supply thefundermental data for reseach henceforth. 2、Materials and MethodThe number of Wister rats is 30 and weight is 180-220g. 30 Wister rats had beenligated on the right hepatic ducts (include middle lobe、right lobe and caudate lobe) tocause partial cholestatic hepafibrosis factitiously and divided in A, B and C groups. Aand B groups had been ligated on the right hepatic ducts both. The different with Aand B is that the time of A group after ligation on the hepatic ducts is 2 weeks and thetime of B group is 1 week.

将实验动物分为A、B、C三组，A组为结扎大鼠右侧胆管(包括中叶、右叶、尾状叶)2周后进行检查。B组为结扎大鼠右侧胆管1周后进行检查，C组行假手术为对照组。A组大鼠麻醉后，腹部备皮，皮肤常规消毒，腹部横行切开，暴露肝脏、胆总管，游离右侧胆管，结扎，然后关腹；C组，仅开腹然后关腹，即假手术组。A、C两组同批实施手术，A、C两组术后1周，B组实施手术，步骤、方法、部位同A组。

According to experimental results and etiopathogenisis and pathology of osteoporosis , and analyzing review investigation on hepcidin、associations of bone metabolism and iron metabolism and relations of ferri ion and calcium ion,we can make conclusions as follows: 1、We applied single manmade intervention approach to induce osteoporosis model in this study, there was no other influential factor. In the termination of test , rats bone density and bone histological anatomy indicated that osteoporosis model was creditable,meanwhile hepatic functional enzyme and hepatic microtome section demonstrated that hepatic function had noci-influence induced by medicinal herbs resource. Accordingly, we could believe that hepatic hepcidin gene expression and serum hepcidin contents can represent internal iron metabolism changes at selected test time. 2、In this investigation, different findings between test group and control group indicated that:according to changes of hepcidin gene expression measured by RT-PCR and changes of serum hepcidin contents determined by ELISA kit , there were correlations between hepcidin and rat osteoporosis model induced by retinoic acid. 3、Under the condition of this investigation,there was a interactive hypothesis as follows: Hepcidin iron metabolism←→ferriion←→calcium ion←→osteoporosis.

根据本次实验结果，对骨质疏松的病因病理的认识及铁代谢与铁调素、骨代谢与铁代谢关系、铁离子与钙离子的关系系列研究的回顾，经分析得出如下结论：1、本研究骨质疏松模型采用单一人工干预方法，没有其他影响因素；实验结束时大鼠骨密度、骨组织学检查表明骨质疏松形成，同时肝脏功能酶和HE切片显示肝脏功能未受药源性产生明显损伤性影响；因此，同期不同时间组大鼠肝脏铁调素基因表达、血清铁调素含量可以代表体内铁代谢不同时间的变化。2、在本研究中，模型组与对照组研究结果的差异表明：铁调素基因RT-PCR变化、血清铁调素ELISA测定含量变化与维甲酸制作的大鼠骨质疏松模型形成有实验相关性。3、在本研究平台下，形成了一个互相影响的假设图：铁调素←→铁代谢←→铁离子←→钙离子骨质疏松。

Methods Using Wistar rats as animal pattem of ADR-induced myocardial injury and sodium selenite as protective agent.The double flourescent staining of apoptosis and expression of transforming growth factor β1 (TGF β1) were examined in liver tissue of animal pattern of ADR-injury by immunohistochemical and TUNEL,and the level of lipid peroxidation in serum was observed.

通过复制大鼠阿霉素性心肌损伤的动物模型，以亚硒酸钠作为保护因素，应用原位末端标记法和免疫组化技术检测大鼠肝细胞凋亡和肝细胞转化生长因子β1表达，并观察了大鼠血清中脂质过氧化含量。

Adult male SD rats were randomly divided into five groups:control group、hypercholesterolemia control group、dosage group with 72.3%,80.5%,96.1% deacetylated flyblow chitosan.The rats were fed with a high cholesterol diet and were administrated flyblow chitosan continuously for 8 weeks at the same time,then the blood lipid and weight were measured.

取成年雄性SD大鼠随机分为5组，分别为正常对照组、高脂模型组、蝇蛆壳聚糖72.3%，80.5%，96.1%脱乙酰度组，给高脂饲料造模的同时连续给予蝇蛆壳聚糖8周，测定大鼠血清总胆固醇、甘油三酯、高密度脂蛋白、低密度脂蛋白含量，同时观察对大鼠体重影响。

The present study explored the effects of morphine on plasticity of spinal cord in three parts as follows: 1. the effects of morphine on dorsal root terminal in spinal lamina Ⅱ of spared root rat---- a quantitative study by FRAP histochemistry; 2. the effects of morpnine on synaptic reinnervation in spinal lamine Ⅱ of spared root rat----an EM quantitative study and 3. the effects of morphine on collateral sprouting of nerve fiber in spared root of rat----an EM quantitative study

本课题从三个方面探讨了吗啡对脊髓可塑性的影响：1、吗啡对备用根大鼠脊髓Ⅱ板层背根终末的影响——抗氟化物酸性磷酸酶组化定量研究；2、吗啡对备用根大鼠脊髓Ⅱ板层突触重建的影响——电镜定量研究；3、吗啡对大鼠备用根神经纤维侧支出芽的影响——电镜定量研究。

M ? min~(-1). All rats were broken the neck after the anaesthesia on the berry to fetch the liver organization, a part was used to test the organization glycogenic content and NO content, another part to prepar paraffin wax section and to measure hepatocyte apoptotic and the expression of Bcl~2 and Bax protein.

按实验设计，所有大鼠均在实施腹腔麻醉术后断头处死取肝脏组织，一部分肝组织用以测定大鼠肝糖原含量和NO含量，另一部分制备肝组织石蜡切片，用以检测大鼠肝凋亡细胞和Bcl-2、Bax的表达。

Purposes: To observe intakes of 〓p-sodium-orthophosphate and 〓P-chromium- orthophosphate at various concentrations by brain gliomas cell; To make thorough inquiry of the inhibition to the SHG44 cell by 〓P-sodium-orthophosphate and 〓P-chromium-orthophosphate at various concentrations; To find out the regularities about the accumulation and elimination in the major organs of rats after inter-brain injection with 〓P-sodium-orthophosphate and 〓P-chromic-orthophosphate; To investigate the therapeutic effects of intert-injection with 〓p-chromium-orthophosphate on the neoplasia transplanted in the brain and hypoderma; To estimate the absorbed dose of the neoplasia body in rats after internal radiation; To study the pathological changes in the rat cells and tissues caused by the radiation exposure from 〓p.

目的：观察人脑胶质瘤SHG44细胞对不同浓度的〓P-磷酸钠和〓P-磷酸铬的摄取；探讨不同浓度〓p-磷酸钠和〓p-磷酸铬对SHG44细胞生长的抑制；揭示〓p-磷酸钠和〓p-磷酸铬在大鼠颅内注射在时全身主要脏器的蓄积与排除规律；观察〓p-磷酸铬大鼠颅内瘤体、皮下瘤体内注射的治疗效果；估算大鼠瘤体组织的吸收剂量；观察〓p照射引起的细胞及组织的病理学改变，为临床脑胶质瘤内照射治疗提供实验依据和吸收剂量计算方法。

Methods: Fifty-five male adult SD rats were randomly divided into a normal control group, a saline control group and a heroin dependence group. Heroin-dependent rat models were set up by subcutaneous injection of heroin and the hypophysis were excised on the 10, 17, 24, 31, and 38 days after models were set up. Immunohistochemical SABC method, imagine analysis, and radioimmunoassay were used in the research.

成年雄性SD大鼠55只，随机分为正常对照组、盐水对照组及海洛因依赖组；皮下注射海洛因建立海洛因依赖大鼠模型，分别于模型建立的第10、17、24、31、38天取垂体组织，应用免疫组织化学SABC法、图像分析法观察大鼠垂体远侧部β-内啡肽表达。

Methods With guinea pig spinal cord homogenate in complete Freund's adjuvant or MBP in complete Freund's adjuvant and Bordetella pertussis vaccine was injected intradermally to induce EAE in Wistar rats. To investigate the suppression of clinical and histological manifestations of the disease when animals were fed with MBP before or after disease induction.

目的 建立实验性自身免疫性脑脊髓炎动物模型，探讨口服自身抗原诱导免疫耐受对大鼠EAE的防治作用方法在普通Wistar大鼠经1次足跖真皮内注射完全弗氏佐剂-豚鼠全脊髓匀浆或完全弗氏佐剂-髓鞘碱性蛋白乳剂加百日咳疫苗诱发EAE疾病；另在大鼠致炎及发病后口服髓鞘碱性蛋白，观察其对EAE的防治作用。

ResultsComparing with gonadectomy Shenyangxu model group, Wenyangyishen Capsules can improve upset excitability of male rat of gonadectomy penis Home News for Overseas Service Department , shorten erection incubation period; The rat increasing gonadectomy male sex attaches weight modulus of sexual organ; Can urge the hypophysis , adrenal gland weight modulus to increase by, weight adjusting the thymus gland , spleen; Elevate rat serum testosterone、Cortisol contents obviously; Be caused by messenger Cortisol the various Shenyangxu model small rat symptom gets laxation disappear even.

结果 与去势肾阳虚模型组比较，温阳益肾胶囊能提高去势雄性大鼠阴茎对外部刺激的兴奋性，缩短勃起潜伏期；增加去势雄性大鼠附性器官的重量系数；能促使垂体、肾上腺重量系数增加，调节胸腺、脾脏的重量；明显升高大鼠血清睾酮、皮质醇含量；使氢化可的松所致阳虚小鼠各项症状得到缓解甚至消失。结论温阳益肾胶囊具有益肾壮阳的功效。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。