大鼠

This paper aims at observing the effect of combined use of calcium antagonistwhich acts on the cell membrane and that acts intracellularly on theportal venous pressure of normal rats and that of the rat model of cirrhosis with portalhypertension, Two experiments were included in this study.

本文旨在观察细胞膜和细胞内钙拮抗剂单独与联合使用对正常大鼠和肝硬化门脉高压大鼠门脉压力的影响。实验分两部分进行：钙拮抗剂对钙离子诱导大鼠门脉压力升高的影响；钙拮抗剂预防及阻止鼠肝硬化门脉高压症的研究。

We use microsurgery vascular anastomosis technique, xenogenic scaffoldfrom vessel and xenogenic small vessels were anastomosed with parasitifer arteryby invaginate at proximal, end while the end to end anastomosis were perfomred in the distal end.

将实验动物分为四个组：①正常对照组：Wistar大鼠5只和日本大耳白兔7只；②异种小血管支架组：Wistar大鼠45只；③异种小血管组：Wistar大鼠45只；④异种宿主组：日本大耳白兔60只，左右耳各1例／只，共120例。

It were analyzed DNA polymorphism in baby rats between outbred Wistar and SD rats with 30 microsatellites DNA loci of PCR amplification. And was breeding baby inbred rats using high and middle of Coefficient of similarity with female SD rats of F0. It was indicated that there were hybrid and polymorphism loci, there weren't homo-gene loci of F2. It were monomorphism homo-gene for 27, rate of homo-gene loci for 90%.

利用PCR扩增30个微卫星DNA位点对封闭群SD和Wistar大鼠交配繁殖的仔代鼠进行微卫星DNA多态性分析，仔代中与母代SD大鼠相似系数高的与中的进行定向交配繁殖。F2代大鼠均为杂合多态的位点，没有纯合位点；到F9代时基因纯合位点达27个，纯合基因位点率为90%。

Using Gαq/11 polyclonal antibody as prole and immunoblot analysis, we measured Gαq/11 contents in left ventricle of rats. There was a significant decrease in Gαq/11 content of CA rats compared with values of control animals after 3 days of operation (P＜0.01). Gαq/11 contents were not modified in left ventricle after 10 and 30 days of stenosis.

以兔抗鼠Gαq/11多克隆抗体为探针，用免疫印迹方法测定了大鼠左心室组织中Gαq/11蛋白的相对含量，结果发现术后3天CA组大鼠左室中Gαq/11含量较SO组明显降低(P＜0.01)，术后10天和30天二组大鼠心肌中Gαq/11含量无差异(P＞0.05)。

Results: Neonatal rats in hypoxic-ischemicgroup behaved restlessness, cyanosis, deep and rapid breath, astasia, lethargy, irritation and spasm; There were local pyknosis, fragmentation, dissymmetry, blur or disappearance of nucleoli, raritas of ground substance in left cerebral cortex; but there was no statistic significance in chinese traditional medicine group and sham group. The expression of HIF-1α mRNA in brain tissue of experimental group and chinese traditional medicine group were higher than that in sham group, especially chinese traditional medicine group at 3d; HIF-1α positive cells were found in cerebral cortex and hippocampus. There were more HIF-1α positive cells in experimental group and chinese traditional medicine group and mainly in vascular endothelial cells.

结果 缺氧缺血后大鼠相继表现为烦躁不安、全身发给、呼吸加深加快、站立不稳、嗜睡、激惹或间断发作的痉挛和抽搐；HE染色显示实验组大鼠左侧大脑皮层出现局灶性神经元核固缩，核碎裂，核仁偏位，不清或消失，基质疏松；中药组大鼠脑组织结构基本接近假手术组，无明显水肿和坏死表现；实验组和中药组大鼠脑组织HIF-1α mRNA表达较假手术组明显增加，尤其是中药组实验第3日者；免疫组化显示实验组和中药组各时间点大鼠大脑皮层和海马区均可见不同程度的HIF-1α表达，明显高于假手术组，阳性表达主要在血管内皮细胞，海马和皮层的锥体细胞亦有HIF-1α阳性细胞分布。

METHODS: 40 rats were randomly divided into four groups:normal group, asthenic spleen group, high-dosage group and low-dosage group with 10 rats in each group, the asthenic spleen rat models were established in group B, C group as well as group D. Rats in group B, group C, group D were fed with normal distilled water, high-dosage of Guizhi decoction and low-dosage of Guizhi decoction respectively, NFAT mRNA, IL-4 mRNA, IFN-γmRNA were determined by PCR.

40只大鼠随机分为空白对照组、脾虚组、桂枝汤大剂量组和桂枝汤小剂量组等4组，每组10只；用规范的脾气虚证大鼠模型造模方法将B组、C组、D组大鼠造模，B组、C组、D组分别用蒸馏水、桂枝汤大剂量、桂枝汤小剂量灌胃，标本采集后用RT-PCR检测NFAT mRNA，IL-4 mRNA和IFN-γ mRNA表达水平。

Results showed:(1) The airway responsiveness of smoke control group was higher than that of normal control group, the airway responsiveness of smoke + exercise group was lower than that of smoke control group;(2) Plasma level of cortisol determined immediately after exercise was higher than that determined before exercise, no significant difference occurs between that determined next day morning after all exercise finished in contrast to that determined before exercise;(3) HE staining showed, there was severe chronic pulmonary inflammatory response in smoke control group, which was reduced in the smoke + exercise group;(4) chronic cigarette smoke downregulated the protein and mRNA expression of BKca in smooth muscles of bronchi and bronchioli, exercise increased the mRNA expression of BKca;(5) chronic cigarette smoke downregulated the protein and mRNA expression of Kv1. 5 in smooth muscles of bronchi and bronchioli, exercise increased both the protein and mRNA expression of Kv1. 5 in contrast to smoke control group in bronchioli and did not effect the expression of Kv1. 5 in bronchi;(6) chronic cigarette smoke induced upregulation of FIZZ1/RELMα in bronchial smooth muscle cells, exercise decreased these effect.

结果：(1)吸烟对照组的气道反应性明显增高，吸烟加运动应激可明显降低大鼠的气道反应性；(2)吸烟运动组运动后血浆皮质醇浓度明显高于运动前，但全部运动结束后次日晨测定的血浆皮质醇浓度与运动前无明显差异；(3)HE染色显示，吸烟对照组肺组织出现明显的慢性炎症反应，吸烟加运动应激组比吸烟对照组炎症反应轻；(4)慢性吸烟可降低大鼠大气道和小气道BKca mRNA和蛋白表达，而吸烟加运动应激可使BKca mRNA表达上调，但对小气道的蛋白表达无影响；(5)慢性吸烟可降低大鼠大气道和小气道Kv1.5蛋白和mRNA表达，在小气道吸烟加运动应激可减轻这种作用，大气道则无作用；(6)吸烟使支气管平滑肌的FIZZ1/RELMα蛋白表达明显增强，而吸烟加运动组的RELMα蛋白及mRNA均明显减弱。

Methods From pregnancy to parturition, we used different magnetic dosage to expose pregnant rats. 10 weeks later, we measured liver and kidney function of filial generation rats and metabolic biochemical indexes of glucide, fat, protein and nucleic acid, then changes of these indexes were analyzed to estimate the magnetic hazard.

方法］用恒定磁场(0.4T)每日0.5、1、2h暴磁处理SD受孕母鼠至仔鼠出生，仔鼠继续饲养至10周，检测仔鼠的肝功能、肾功能以及糖类、脂类、蛋白质、核酸代谢的相关生化指标，据此分析磁场对大鼠生理有无影响，判断磁场是否对大鼠有害。

Had rarely or never used protective;(11) MiceGrayAuto had realized the automatic processing of video data from the sexual preference observation system of rats, and MiceGrayManu had accomplished the analysis of images of sexual behaviors of rats;(12) Peripheral given of high dose androgen to female rats before its brain sexual differentiation had caused masculinization and defemininization in female rats, therefore led to homosexuality, and based on the results, we had established the female rat homosexual model;(13) Peripheral given of estrogen to male rats before its brain sexual differentiation had little effects on the sexual preference and sexual behavior of adult rats;(14) Peripheral given of estrogen antagonist to male rats before its brain sexual differentiation had markedly reduced masculine sexual behavior and interfered male sexual preference, but had no effect on feminine sexual behavior, and based on such results, we had established the male rat homosexual model, however the model have to be improved for better sensitivity;(15) The volume of sexually dimorphism nucleus of the preoptic area of the high dose androgen-treated female rats was significantly greater, while on the other hand the volume of AVPV of which was significantly lesser, than the female rat control.

并且有17.79％的男性同性恋者在1年内与异性保持着性关系。但在与异性阴道性交时，绝大多数（92.51％）偶然或不使用安全套；(11)MiceGrayAuto软件实现了大鼠性偏爱视频数据自动处理功能，MiceGrayManu软件实验了大鼠性行为图象分析功能；(12)大脑性分化前高剂量的雄激素有促雌鼠雄性化和去雌性化的作用，并表现为同性性倾向，建立了雌鼠同性恋模型；(13)在雄鼠大脑性分化前外周给予雌激素对成年后的性偏爱和性行为未产生明显影响；(14)在雄鼠大脑性分化前外周给予雌激素拮抗剂明显削弱雄性性行为，阻碍了雄性性偏爱，但雌性行为无明显变化，雄鼠的同性恋模型还有待进一步调整和改进：(15)高剂量雄激素处理的雌鼠SDN-POA体积明显大于对照雌鼠组，而AVPV体积明显小于对照雌鼠组。

MATERIALS: Totally 270 healthy unpregnant SD rats were obtained, including 180 males with body mass of 190- 210 g, and 90 female rats with body mass of

材料：健康未生育的sD大鼠270只，雌性大鼠180只，体质量190-210 g，雄性大鼠90只，体质量225～275 g，鼠颗粒饲料喂养，常规饮水，自然光照，室温25℃。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。