大鼠

During this years, TCM has a prominent effect on Dysfunctional Dyspepsia,so we study the effect of SiNiSan of soup on the Dysfunctional Dyspepsia sharply from the ability of stomach emptying and the ability of intestines pussing on the normal Kunming rats by the coloring matter,and the chang of the nerve fiber, nerve qualitative, on the stomach organize of normal SD ratsMethods:In the experiment, the thirty Kunming rats, whose avoirdupois are about twenty grammes,and thirty S. D rats, whose avoirdupois are about two hundred grammes,were randomly divided into three groups, 0. 9yS group, SiNiSan of soup group and Mosapride Citrate group.

本课题通过对正常小鼠胃内葡聚糖蓝-2000残留量、小肠内葡聚糖蓝-2000推进率，来研究四逆散对小鼠胃排空、小肠推进的影响；通过采用正常大鼠肠道气囊法，记录正常大鼠用药后肠收缩幅度和频率的变化；采用医学彩色图象全自动分析系统，分析用药后正常大鼠胃窦组织中NOS阳性神经元分布面积和酶活性的变化；采用硝酸酶还原法和比色法，检测用药后正常大鼠胃窦组织中NO和TchE的含量变化，通过以上几个方面，研究四逆散的促胃肠动力作用及其作用机制。

METHODS: Adjuvant-induced arthritis rats were treated with acupuncture therapy, Chinese herb treatment or both in combination for one week, then the density of TMPH in heart, liver, kidney, and hind paw was determined by HPLC. The cAMP level in the heart and liver was also measured by radioimmunoassay. The blood changes were detected through the test of blood rheometer.

以佐剂性关节炎大鼠为模型，观察单纯针刺法、单纯药物法及针药结合法分别连续治疗一周后，用HPLC法测定各组大鼠心脏、肝脏、肾脏及病灶局部组织中盐酸川芎嗪的含量；用放射免疫法测定各组大鼠心脏、肝脏中cAMP水平；用血液流变仪测定各组大鼠的血液流变学变化。

Rresults from those studies can be summariazed as follow:(1) A preliminary study no correlationship between the UV-VIS reflection spectrum of tomato fruit surface and the lycopene amount of tomato fruit was undertaken. Based on observation from this study, a damage-free and in-situ detection technique of lycopene amount from tomato was developed with UV-VIS reflection spectroscopy;(2) The geometrical isomers of lycopene were separated successfully on C30-HPLC-PDA-ELSD. The absorption coefficients of Z-isomers were consequently dedermined. The geometrical isomer composition of lycopene could therefore be assessed. Those efforts formed a solid base to identify the natural product from synthetic compound of lycopene;(3) Lycopene resource from the fruit of Autumn oliver was proved to be available for industrial application. An extraction methos of lycopene from the fruit of Autumn oliver by supercritical carbon dioxide was developed at laboratory scale;(4) A method to extract lycopene by supercritical liquid with improved efficiency was developed; Data from this investigation suggested that a proper raw material pre-extraction process was important for a better extraction efficiency;(5) Variation in the geometrical isomer composition of lycopene during its metabolish in rat was assessed. A large amount of Z-isomers were observed. This variation took place in serum;(6) The absorption rate and accumulation in serum of natural lycopene in rat were assessed;(7) Ability to quench singlet oxygen by different geometrical isomers of lycopene was compared;(8) It was detected that whether natural lycopene had functions to regule blood-lipids and LDL antioxidation;(9) A C31 degradation piece of lycopene was found in the prostate gland of rat.

研究结果包括：[1]应用UV-VIS反射光谱法建立了番茄果实中番茄红素含量的无损伤、现场检测技术；[2]应用C30-HPLC-PDA-ELSD技术实现了对番茄红素几何异构体的分离，测定了不同顺式异构体的吸光系数，建立了天然番茄红素几何异构体组成的检测方法，为区分和鉴定人工合成品和天然产物奠定了基础；[3]证实了秋橄榄果实中的番茄红素资源具有工业开发价值，并建立了超临界CO_2萃取的实验室方法；[4]探索了提高番茄红素超临界流体萃取效率的方法，证明了物料前处理对提高萃取效率起了极其重要的作用；[5]观察了番茄红素在大鼠体内几何异构体组成的变化情况，发现了大量顺式异构体的存在，并证实了几何异构体组成在血清中发生了显著变化；[6]测定了天然番茄红素在大鼠体内的吸收率和在大鼠血清中的动态积累规律；[7]比较了不同番茄红素几何异构体淬灭单线态氧的能力；[8]检测了天然番茄红素是否具有调节血脂作用和抗低密度脂蛋白氧化的功能；[9]首次发现大鼠前列腺内存在番茄红素C31降解片段，并证实其仍具有淬灭单线态氧的能力。

They were implanted thecannulas into the right striatums of rats according to the followingcoordinates:A:+0.2mm,L:+3mm,V:3.5mm and the microdialysates were collected andmeasured by HPLC-ECD 24h later. At the second step, 10 rats were divided intocontrol and model groups randomly.

第2批10大鼠在BMD和HPLC-ECD实验方法建立的基础上，随机分为对照组和模型组各5只，按照前述坐标定位至大鼠右侧纹状体，然后向模型组大鼠右侧纹状体内注入6-OHDA 12μg/4μl(用含0.1％抗坏血酸的0.9％氯化钠溶液配制)制作急性PD模型，对照组大鼠同一部位注射等量生理盐水。

All rats were administered by free drinking, the normal control group were treated by drinking water in 3 months, 45 d group was performed drinking water in 1.5 months and then revival liquid of quantum in 1.5 months, and the 90 d group was administrated the revival liquid of quantum in 3 months.

方法］ 取雌性Wistar大鼠36只，按体重随机分为对照组、量子复活液45 d组和90 d组；以自由饮用方式给药，给予对照组大鼠普通自来水喂养90 d，给予量子复活液45 d组大鼠普通自来水45 d后，再给予量子复活液喂养45 d，给予量子复活液90 d组大鼠量子复活液90 d。

METHODS Thirty six Wistar female rats used in experiment were randomly divided into three groups: the normal control group, 45 d group and 90 d group with the revival liquid of quantum. All rats were administered by free drinking, the normal control group were treated by drinking water in 3 months, 45 d group was performed drinking water in 1.5 months and then revival liquid of quantum in 1.5 months, and the 90 d group was administrated the revival liquid of quantum in 3 months.

方法］ 取雌性Wistar大鼠36只，按体重随机分为对照组、量子复活液45 d组和90 d组；以自由饮用方式给药，给予对照组大鼠普通自来水喂养90 d，给予量子复活液45 d组大鼠普通自来水45 d后，再给予量子复活液喂养45 d，给予量子复活液90 d组大鼠量子复活液90 d。

The native type Ⅰ collagen was extracted from rat-tail tendons.

实验材料：H460细胞系由解放军总医院惠赠；Wistar大鼠，体质量150～200g，实验中的胶原仅从大鼠的尾部肌腱提取，该大鼠来源为其他科室实验过程中未做任何处理的大鼠。

Experimental Center of National Level One, Hubei Traditional Chinese Medica l College, Wuhan 430061）Abstract To investigate the protective effects of Jiaweisinisa n on acute liver injury induced by intraperitoneal injection of D-ga lac-tosamise, serum superoxide dismutase, malondialdehyde , glutathioe contents were measured. The pathological changes of liver wer e observed simultaneously. The results showed that JWSNS could decrease serum MD A content, but increase serum SOD and GSH activities in acute liver injury induc ed by D-GalN. It was also found that JWSNS could alleviate the degeneration and necrosis of the hypatocytes.

为研讨加味四逆散防治肝损伤的作用机理，通过动物试验，先用加味四逆散预防给药，再采用D-氨基半乳糖造成大鼠急性肝损伤模型，然后检测大鼠血清超氧化物岐化酶、丙二醛、还原型谷胱甘肽水平，同步观察该方对急性肝损伤大鼠体内脂质过氧化反应及肝脏病理改变的影响，结果表明该方可明显升高大鼠血清SOD、GSH水平，降低MDA水平，肝脏病理学检查亦表明该方使肝细胞变性坏死程度减轻。

The results showed that (1) COX-2 mRNA and protein existed in the mature male rat testis. COX-2 was localized in spermatocytes by immunohistochemistry.(2) COX-2 also existed in adult man testis. COX-2 protein was positively stained in spermatocytes and Leydig cells.(3) 2 weeks after administration of rofecoxib, the level of testosterone in the whole testis reached its lower values, being only 50% of control values. However, testosterone level recovered during 4 weeks. After such treatment, histologic examination of these testes showed atrophy of the seminiferous tubules and maturation arrest of spermatogenesis.(4) 2 weeks post-EDS, expression of COX-2 decreased significantly (P.005), in comparison with vehicle-treatment control. 4 weeks after treatment, a new generation of fetal like Leydig cells repopulated in the testicular interstitium resulting in COX-2 expression partially recovered. Although the expression of COX-2 mRNA and protein are enhanced at 8th week after using external testosterone, it wasn't significant higher than control group.

实验研究证明：(1)正常成年雄性大鼠睾丸组织中COX-2在mRNA和蛋白质水平均存在表达，免疫组织化学结果显示COX-2定位于曲细精管内的生精细胞；正常成年男性睾丸组织中同样存在COX-2表达，免疫组织化学结果显示COX-2定位于曲细精管内的生精细胞和Leydig细胞；(2)服用特异性COX-2酶抑制剂rofecoxib 2周后，实验组大鼠睾丸组织内睾酮的含量减少，为正常对照组的50％；持续用药4周后睾丸组织内睾酮浓度逐渐恢复至正常水平；COX-2酶活性降低后病理组织切片显示睾丸内曲细精管萎缩，生精紊乱，持续用药4周时影响最明显；(3)注射特异性Leydig细胞杀灭剂EDS 2周后，实验组大鼠睾丸组织内睾酮浓度降至极低水平时，COX-2的蛋白和基因表达水平也显著低于正常空白对照组（P.005）；使用EDS后第4周，睾丸组织中的睾酮浓度逐渐回升，同样组织中COX-2蛋白表达和mRNA表达水平也相应提高接近正常水平；使用EDS后4周开始给予外源性睾酮，6周和8周时COX-2表达水平的绝对值虽有提高，但与正常大鼠空白对照组比较并无显著性差异，说明外源性雄激素刺激睾丸合成COX-2的作用并不明显。

Methods Total RNA was isolated from normal choroid and choroid tissues with neovascularization induced by krypton laser photocoagulation in Brown Norway rat.

对氪激光诱发BN大鼠脉络膜新生血管的脉络膜组织和正常BN大鼠的脉络膜组织进行总RNA的提取，将纯化后的mRNA进行逆转录制备杂交探针，应用含有5705条OligoDNA的表达谱芯片对氪激光诱发BN大鼠脉络膜新生血管的脉络膜组织和正常BN大鼠的脉络膜组织进行差异表达谱分析。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。