大黄素

Methods: fifteen sd rats were randomly divided into five groups (n=3), and adrenalectomy was performed and anterior pituitary specimens were obtained after a week. anti gap 43 antibody and anti adrenocorticotrophic hormone, thyroid stimulating hormone, luteinizing hormone, prolactin and growth hormone antibodies were used in the double immunohistochemical staining.

将成年雄性sd大鼠15只随机分为5组(n=3)，手术切除大鼠双侧肾上腺，1 wk后取垂体前叶标本分别进行抗gap 43与抗促肾上腺皮质激素、促甲状腺激素、黄体生成素、催乳素、生长激素抗体的免疫组织化学双标染色，观察gap 43 li神经纤维与各腺细胞之间的关系。

To prevent side effects of cantharidin, cantharidin analogues, disodium cantharidate and norcantharidin were discovered.

斑蝥素入中药可追溯至2000年以前，主要的品种是南方大斑蝥及黄黑小斑蝥，入药部位为乾燥虫体。

Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上，开展了如下未见文献报道的创新性的研究工作：(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究，建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法，与以前的报道相比，灵敏度和分析时间均有很大改善；(2)使用荧光素钠作为背景荧光试剂，建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法，扩大了激光诱导荧光检测的应用范围，对难衍生化合物的分离分析提供了一种新思路和新途径；(3)以荧光素钠作为背景荧光试剂，建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法；(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物，为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。

Safrole oil is nowadays and will be still the main raw material for heliotropine manufacture.Because catalyzation method with potassium hydroxide has merits of cheap material and simple technique , it becomes the sole method to produce isosafrole in domestic factories.

由于氢氧化钾催化黄樟油素异构化法具有原料方便、工艺简单特点，故它是国内工业生产异黄樟油素的唯一方法，但存在收率低(收率小于90％)、污水量大的缺陷，目前一直未解决。

By use of AB-8 macroporous resin and with 10％ gardenia yellow as adsorptive solution, selecting proper adsorptive and desorptive conditions, crocin and geniposide can be separated effectively, the purified gardenia yellow whose colority increases from 10 to 320 and OD ratio decreases from 2. 31 to less than 0.4 answers for the standard of gardenia yellow of high quality.

采用AB—8大孔树脂，以10％的栀子黄溶液上柱，选择合适的吸附、解吸条件，栀子黄的色价可从10提高至320，OD值比率也从2.31降至0.4以下，藏花素和栀子甙均达到了有效地分离。

In situ hybridization using biotinylated POMC cRNA probe made it possible to localize POMC mRNA in the rat ovary. POMC mRNA predominantly existed in corpora lutea of diesturs, pregnant and pseudopregnant rat ovaries. POMC containing cells scattered in the ovarian intertitium were seen. The amount of POMC mRNA in granulosa cells was negligible.

采用生物素标记的POMCcRNA作为探针，进行原位杂文，研究POMCmRNA在大鼠卵巢中的分布，实验表明，POMCmRNA主要分布于间期，妊娠和假孕大鼠卵巢黄体中，间质细胞也可见少量POMCmRNA，而卵泡颗粒细胞中POMCmRNA极少。

The result showed that radices puerarire total flavone could adjust the insulin, the glucagon and the excretion of growth hormone, accelerate fat dissolution, raise FFA in blood and muscle, and economize the consumption of glycogen.

研究结果显示：葛根总黄酮可调节运动大鼠胰岛素、胰高血糖素和生长激素的分泌，促进脂肪分解，提高大鼠血液游离脂肪酸和骨骼肌FFA的含量，节省糖原的消耗。

AIM: To review the 10 kinds of macroporous resin＇ s absorption of total flavnoids to screen out the one which could separate the Baicalin, Baicalein, Wogonoside, Wogonin simultaneously.

目的：考察10种型号的大孔吸附树脂对黄芩总黄酮的吸附性，优选出分离黄芩苷、汉黄芩苷、黄芩素、汉黄芩素的大孔吸附树脂。

Green eggs with a shell of the egg is different from other nutritional content and structure of the green shell of the egg yolk than Cao Ji 8% of the large egg yolk, egg white thick, easily digested and absorbed by the body, egg yolk pigment Roche up to 13 About class (ordinary egg yolk pigment Roche for the general level 4 -5), lecithin, a living -A, B, E, amino acid content than the 5-10 times higherthanordinary eggs, the yolk of the egg ball ammonia β White and γ-globulin were higher than other kinds of chicken, the best is good tonic, Green Egg high-quality, nutritious, high-selenium, high zinc, high-PC PE, high Amino acid, high-vitamin A, E, the high non-saturated fatty acid, low-fat, low cholesterol, high Flavin light, the lightpigment αβγ and unknown immune globulin antibodiesanddiseaseresistantfactorisextremely desirable Health food, the scientists as "the ginseng eggs."

绿壳蛋具有不同于其它蛋品的营养结构和含量，绿壳蛋的蛋黄比草鸡蛋黄大8%，蛋清稠，极易被人体消化吸收，蛋黄色素可达罗氏13级左右（普通鸡蛋的蛋黄色素一般为罗氏4级-5级），卵磷脂、维生素A、B、E、氨基酸的含量比普通鸡蛋高出5-10倍，蛋黄中的氨β球蛋白和γ球蛋白均高于其它鸡种，是上等滋补佳品，绿蛋蛋品质高，营养丰富，含高硒、高锌、高卵磷脂脑磷脂、高氨基酸、高维生素A、E、高不饱合脂肪酸，低脂肪、低胆固醇、富含光黄素、光色素αβγ球蛋白和未知免疫抗体和抗病因子，是极为理想的保健食品，被科学家誉为&鸡蛋中的人参&。

Results show that maximum activities of LiP and Lac reach 11.3 u/g at the 12th day and 0.0992 u/g at the 15th day, rapidly degraded during 10～20 d, and the total loss rate of dry corn stalk is 18.94% after cultivation for 25d. The relative contents of celluloses and hemicellulose are decreased from 27.1% to 13.1% and from 20.3% to 11.9%, respectively after enzymolysis for 6 d.

黄孢原毛平革菌培养12 d时木素过氧化物酶酶活达到最大值11.3 u/g，15d时漆酶酶活达到最大值0.0992 u/g，秸秆降解集中在10～20 d，第25d时菌解结束，干基总损失率为18.94%；再经过6 d的酶解，纤维素、半纤维素的相对含量分别从27.1%、20.3%下降到13.1%、11.9%。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。