大量

In othor way, we use RT-PCR to observe the secretion of mRNA to compare with the balance of TH1/TH2 in spleen, so that we can see if we treat with cystamine can apparently raise the ratio of TH1 and also raise mRNA of CD25/ Foxp3, but reduce mRNA of CD40L. As a result of kidney which is the important secondary immunity organ discusses cystamin whether is the important control action or not to regulate in kidney then to be able to improve illness.

另外，透过了反转录聚合链锁反应(reverse transcription-polymerase chain reaction， RT-PCR)观察spleen中CD8/CD4 maker的mRNA表现量比较TH1/TH2 balance的倾向，发现经过胱胺处理后的确TH1相关的CD8 maker的mRNA比例量有大幅度上升，间接证明SLE倾向於TH2有被反转，而在Treg的特异性 maker方面CD25/ Foxp3之mRNA之比例也有大量上升，T cell的活性化maker CD40L也减少了mRNA的表现量，同时证实了SLE免疫倾向的反转，及发炎情况的减少及Treg cell的大量诱发。

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果：1合成的CS-PAA磁性聚合物微球呈球形，大小均一，TEM测定其干态下粒径为80nm左右，磁化曲线证实具有超顺磁性，具有一定的PH敏感性，固载表柔比星后，水溶液性状稳定，无沉淀物，DLS测定直径约200nm左右，测定载药率为15％，较文献报道高，包封率为20％。2肉眼观察试验组大鼠膀胱表面呈褐色，可见大量的Fe粒子聚集，普鲁士兰染色法显示，试验组大鼠膀胱壁内有大量的Fe粒子，分布至膀胱壁全层，与对照组大鼠相比，试验组大鼠的肝、脾内的Fe粒子聚集量明显降低；HPLC测定结果与Fe染色相同；高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高，其他组大鼠血生化指标没有明显变化。3MTT法发现，高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组，FCM发现试验药物组可引起明显的肿瘤细胞凋亡，试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C，试验组细胞Bcl-2蛋白减少，Bax蛋白变化不明显，Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P＜0.01)，其中高剂量组的抑制率最高。

By the cooperation of 8-MOP/UVA, the photoaging-characteristic biological markers of the dermic fibroblasts in vivo and in intro were changed as follows:① with a permanent switch of mitotic to stably postmitotic phentypes, fibroblasts displayed growth suppression and morphological changes of cell senescence;② increasing expression of SA-β-galactosidase;③ increasing expression of p16 protein;④ continuous up-regulation of mRNA of MMP-1 and MMP-3, while the protein of TIMP-1 was only slightly induced;⑤ high levels of the 4977bp deleted mtDNA accumulated in dermis and cultured fibroblasts, and large accumulation of a A→C base transversion of 414 position of 〓 of human mtDNA control region for replication of cultured fibroblasts also.

2.8-MOP/UVA作用下，体内外真皮成纤维细胞生物学特性出现具有光老化特征性的改变：①细胞由具有分裂活性的分裂表型转化为不具有分裂能力的分裂后表型，形态学出现细胞衰老的相应改变；②SA-β-Gal表达增加；③p16蛋白表达增加；④MMP-1、MMP-3 mRNA持续表达而TIMP蛋白表达仅被轻微诱导；⑤mtDNA 4977bp缺失大量累积，培养成纤维细胞mtDNA复制控制区〓片段414碱基迅速出现大量A→C的点突变。

In C. indicus, the infected mechanism correlated with season. The infusoriform larvae of dicyemid mesozoan dispersal from the host to sea water in the autumn and winter, then entering host in the spring and summer. The change of dicyemid life cycle stage in the C. indicus kidney correlates with the sharp decrease of infection intensity. However, the dicyemid infection in Octopus marginatus correlates with body size, not with season.

在C。 indicus之分析中可发现，中生虫之感染与季节有关，有性幼体於秋、冬季自寄主章鱼体内扩散至外界，并於春、夏季大量进入寄主，接著在寄主体内发展，每一次生活史之时期转变，伴随著感染强度之骤降，直至成熟之菱形体时期，大量有性幼体出现肾腔中，至秋季有性幼体随寄主尿液排至外界，下一个生活史之循环接著开始。

The soil infiltration system for sewage treatment consists of septic tank and soil infiltration system and has the advantages of single equipment,less investemt,convenient operation and management,low energy consumption and better purifying effect,being specially suitable for the region where sewage pipeline is incomplete and domestic sewage discharges dispersively.

现代传统广泛应用的污水处理系统是污水处理厂，在一个复杂和大量耗能的机械设备内通过物理、化学和生物的过程对污水进行处理。然而，这种处理系统不仅耗资巨大而且在消耗大量化石燃料的同时，还会产生新的固体废物和污泥[1]。

After finish the digestion, then flow back to the blood.also, during the period of liver and gallbladder concertrate in the hepatoenteral circulation,the bile acid can drain massively with the excrement.and more seriously after adding the fat feed stuff.if this condition go on, the fat cannot be absorbed in time and the energy will be wasted and cause some disease and etc.but after adding the appropriate exogenetic emulsifier,the question can be solved.

作为正常的机体，胆汁酸由肝脏分泌，进入肠道，在完成消化，回流入血液，又在肝脏和胆囊重新富集的肝肠循环的过程中，会经过粪便大量流失。在脂肪含量较高的饲料配方下，流失更为严重。日肝脏分泌量不足弥补流失量，会造成脂肪消化的阻滞，能量的浪费，且易诱发肝胆代谢紊乱等各种疾病。得不到消化吸收的油脂，在肠道内被噬油微生物降解，使噬油微生物类群大量增殖，打破原来肠道内微生态平衡，产生腹泻等菌群失调症，以上的问题，都是由于脂肪乳化作用不足和脂肪代谢失调造成的。在补充了合适本物种须求的外源性乳化剂后，问题可得到解决。

There was large amount of ferrous sulfide deposited on the top of the reactor. The strong exothermal effect during the oxidation of the deposited carbon and ferrous sulfide caused 'hot spot' near the top of the reactor, which makes the temperature runaway easily.

结果表明，由于加氢脱硫反应器底床顶部沉积了大量的硫化亚铁，氧化再生过程中，催化剂上的积炭，沉积的硫化亚铁等均放出大量的热量，使反应器顶部产生热点，并可能造成飞温。

Crossing seems to be a bit floaty and the shooting has completely changed again so it needs a good practise again. Shooting like PES 2008 will result in you scoring 0. This isn't so much a bad thing but a nuisance.

首先是觉得传中有点飘，而且射门方式与2008完全不同，假如你还是采纳2008的方式去射门那你将会吃个鸭蛋，这需要玩家重新进行大量练习好好了解一番才行，尽管这个改变是好的，可是毕竟大量的练习会让人厌烦，而在一球成著名模特式中还是没方法创建守门员或后卫，这让我感到遗憾。

Crossing seems to be a bit floaty and the shooting has completely changed again so it needs a good practise again. Shooting like PES 2008 will result in you scoring 0. This isn't so much a bad thing but a nuisance.

首先是觉得传中有点飘，而且射门方式与2008完全不同，如果你还是采用2008的方式去射门那你将会吃个鸭蛋，这需要玩家重新进行大量练习好好熟悉一番才行，虽然这个改变是好的，但是毕竟大量的练习会让人厌烦，而在一球成名模式中还是没办法创建守门员或后卫，这让我感到遗憾。

Crossing seems to be a bit floaty and the shooting has completely changed again so it needs a good practise again. Shooting like PES 2008 will result in you scoring 0. This isn't so much a bad thing but a nuisance.I am disappointed that Bayern have gone and the master league hasn't changed - even the menus are the same.

首先是觉得传中有点飘，而且射门方式与2008完全不同，如果你还是采用2008的方式去射门那你将会吃个鸭蛋，这需要玩家重新进行大量练习好好熟悉一番才行，虽然这个改变是好的，但是毕竟大量的练习会让人厌烦，而在一球成名模式中还是没办法创建守门员或后卫，这让我感到遗憾。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。