大肠杆菌病

Protoscoleces of cyst were recovered from the brain of sheep naturally infected with coenurus of Taenia multiceps and total RNA was extracted.T.multiceps thioredoxin peroxidase cDNA fragment was amplified by RT-PCR from the total RNA,ligated into pMD18-T vector and sequenced.

从自然感染的病羊脑内采集多头蚴原头节，提取总RNA，采用RT-PCR技术扩增多头带绦虫硫氧还蛋白过氧化物酶基因片段，PCR产物连接到pMD18-T载体，转化至大肠杆菌DH5α后测序，发现克隆到的TmTPx基因片段开放阅读框为453bp，编码150个氨基酸。

In the animal model of rabbits with Ying-Xue-Fen syndrome caused by the endotoxin of colon bacillus,the number and aggregation rate of platelet are obviously lower.

大肠杆菌内毒素所致的家兔温病营血分证动物模型，其血小板数量和血小板聚集率明显降低。

The technology for purifying triple serum antibody and yolk antibody against Newcastle disease, colibacillosis and paretyphus avium in chicken has been established.

对抗鸡新城疫ND、大肠杆菌病AC、副伤寒PA三联血清抗体和抗鸡新城疫高免卵黄抗体进行了提纯工艺研究。

In an epidemic investigation on some broiler farms in Yanggu , the disease of the ducks was diagnosed as colibacillosis based on the clinic symptoms , pathogenic changes and pathogen isolation and identification.

对山东阳谷县养鸡场疾病进行调查，根据临床症状、病理变化及病原分离鉴定，确诊为大肠杆菌病。

Susceptibility test ,effective dosage test, safety test ,clinical therapeutic and preventive test and comparative therapeutic test with western medicine were all done by using self-made compound recipe of Chinese herbal medicine in colibacillosis of egg chickens.

用自拟中草药复方对蛋雏鸡大肠杆菌病进行了病原菌体外药物敏感试验、中西药疗效对比试验及临床治疗和预防试验。

Key words: antagonistic actinomycete;fermenting liquor;chicken;colibacillosis;efficacy trial

拮抗放线菌；发酵液；鸡；大肠杆菌病；疗效试验

Methods The coding gene of Sj32KD antigen was amplified from the cDNA library of the adult parasite by PCR and cloned into the prokaryotic expression vector pET28a to construct a re- combinant plasmid Sj32-pET28a.

应用PCR方法克隆Sj32KD抗原基因，并在大肠杆菌系统中进行表达，利用His亲和层析方法纯化融合蛋白，并应用重组的Sj32KD抗原检测羊日本血吸虫病。

Result3 strains of endophytic fungi had growth peak when the pH value was 6.5-7.5,and could grow within pH of 4.0-8.5. Their acid-alkali adaptability was strong,in which the P-3 adaptability was the strongest. P-1and P-2 had the stronger inhibition to the Bacillus subtilis,Escherichia coli and Staphylococcus aureus,and P-3 only had the little inhibition to Escherichia coli. The 3 endophytic fungi all had inhibitory effect on Podosphaera leucotrlcha,Alternaria mali,and Gymnosporangium yamadae Miyabe,in which,P-1 had the better inhibitory ability.

结果3株内生真菌在pH值6.5～7.5出现生长最高峰，在pH值4.0～8.5都可以生长，酸碱适应性很强，其中P-3的适应性最强。P-1和P-2对枯草芽孢杆菌、大肠杆菌、金黄色葡萄球菌都有较强的抑制能力，而P-3只对大肠杆菌有很微弱的抑制能力。3株内生真菌对苹果锈病病原菌、苹果白粉病病原菌、苹果落叶病病原菌都有抑制作用，其中P-1的抑制能力较强。

The Escherichia coli is the most common pathogenic bacilli both on the medicineand the clinical veterinarian, the pathogenic can cause young pig"s yellow diarrheaand white diarrhea,and pig"s hydropsy, and so on.

大肠杆菌是医学和兽医临床上最常见的一种病原菌，其致病性株可引起仔猪黄痢、白痢、猪水肿等病。

Coli. The outer membrane protein were extracted from 38 strains of avian E. coli which were isolated from the dead chickens of chicken breeding farms in 3 areas of Baoding, Qiuhuangdao and Beijing by using ultrasonic cleaving and N-Lauroyl Sarcosine Sodium, and OMP typing was done by SDS-PAGE.

方法］对分离自保定、秦皇岛和北京3个地区规模化养鸡场的病死鸡体内的38株大肠杆菌，采用超声波裂解和N-十二烷基肌胺酸钠进行外膜蛋白提取，利用SDS-PAGE进行Omp分型。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失