大肠杆菌溶素

But we have seldom found the reports about it in China; So writer summarized some literature more than twenty years; name,classification and family of hemolysin have been written, especially three of representative species:α-toxin, Streptolysin O and HlyA; Nowaday, A relationship between the hemolysin and pathopoiesis of bacterium is obvious.

另外，细菌溶血素的英文命名并不统一，检索时容易造成漏检或误检；通过查阅大量英文文献资料，归纳汇总近20年报道的溶血素研究进程；该文详细列出了细菌溶血素名称、分类以及各溶血素家族列表。并且分别介绍了其中三种典型的穿孔毒素：葡萄球菌α-毒素、链球菌溶血素-O、埃希氏大肠杆菌溶血素HlyA。

Objective: To contructre combinant plasmid pET28a/hES and coexpress human Endostatin and predhPK-5 in E. coli. Methods: mRNA from human liver tissue was exracted, and the Endostatin gene was amplified by RT-PCR.

目的构建人内皮抑素原核表达质粒pET28a/hES，并与简化人纤溶酶原饼环区(predigested human PlasminogenKringle5，predhPK-5)在大肠杆菌中实现共表达。

Here, we demonstrated in this work that CsrA performs a negative regulator of the cel expression. cel, encoding lysis protein (LysE7), is located downstream of the colicin E7 structural gene in the ColE7 operon. Lysis protein was essential for colicin release and causes a decline in culture turbidity as well as lethality of the host cell when overproduced. Western blotting analysis of the level of LysE7 in the wild-type and csrA mutant strains was examined.

在本论文中，我们进一步发现到CsrA对於cel 基因的表现具有抑制的现象。cel 基因位在质体ColE7-K317上的E7大肠杆菌操纵子，可以制造出溶菌蛋白质(lysis protein， LysE7)，协助大肠杆菌素及免疫蛋白质的复合体运送至菌体外；但当菌体产生过多的溶菌蛋白质，则会导致宿主细胞的死亡，所以溶菌蛋白质的表现必须受到严密的调控，避免菌体产生过量，而CsrA则是目前第一个被发现到能调控溶菌蛋白质表现的因子。

The most pathogenic isolate, VIB 645, contained two closely related hemolysin genes, which were cloned and sequenced. In this study, vhhA was cloned into an expression plasmid pET-24d.

本研究将哈维氏弧菌的溶血素基因vhhA克隆到大肠杆菌的表达载体pET-24d，VHH溶血素作为一种带六个组氨酸的融合蛋白在大肠杆菌表达菌株BL21(DE3)中得到了过量表达，重组大肠杆菌及其培养上清液在鱼血平板上有很强的溶血活性。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。