大肠杆菌

Using Pseudomonas syringae and Pantoea ananas , the dominant ice nucleation active bacteria strains from Yunnan province, as receptors of genetic engineering recombination, and Escherichia coli S17/pZJ25∶∶Tn5 as donator. By transposon Tn5 induced mutants method, the Tn5 was inserted and integrated into the genome of wild type INA + bacteria. INA + mutant as INA - when the linear continuous of INA + gene destroyed.

以云南省冰核细菌的优势种类丁香假单胞菌Pseudomonassyringae和菠萝泛菌Pantoeaananas为重组基因工程菌的受体菌，采用Tn5转座子诱变技术，以大肠杆菌EscherichiacoliS17/pZJ2 5∶∶Tn5作为转座子诱变的供体菌，使Tn5插入并整合到冰核细菌基因组中，使冰核基因的线性连续性被破坏，失去冰核活性。

Recombinant plasmid pFf-22-Trp operon was successfully constructed, and both the anthranilate synthase and tryptophan synthase activities of expressed product increased. It laid a foundation of construction of recombinant E. coli strain for production of tryptophan in a large scale.

已成功构建了重组表达质粒pET-22b-Trp operon，邻氨基苯甲酸合成酶和色氨酸合成酶的活性在大肠杆菌中得到了提高，为高产色氨酸基因工程菌的构建奠定了基础。

The complex can improve the antibiosis activity to Escherichia coli.

配合物能提高配体对大肠杆菌的抗菌活性。

It appeared that in that country the disease was caused by an antibiotic-resistant strain of E.

在那个国家，此病是由一株抵抗抗菌素的埃希氏大肠杆菌菌株引起，由公猪传播的。

It appeared that in that country the disease was caused by an antibiotic-resistant stra in of E.

在那个国家，此病是由一株抵抗抗菌素的埃希氏大肠杆菌菌株引起，由公猪传播的。

It was found that the fusion protein has good antigenicity by Western-blot.

Western印迹分析结果说明该基因在大肠杆菌中的融合表达产物rSjGcp具有良好的抗原性。

Western blotting showed that the recombinant protein had good antigenicity.

Western印迹分析结果说明该基因在大肠杆菌中的融合表达产物rSj423具有良好的抗原性。

In order to define the function of this protein, P6 was expressed in Escherichia colt cells and antiserum against P6 was successfully prepared.

为进一步研究该蛋白的生物学功能，在大肠杆菌中表达了RBSDV基因组片段6编码的P6蛋白并制备了相应的抗血清。

Results HlyA436 was successfully expressed in prokaryotic expression system, have elicited high titer antiserum and has certain protective efficacy.

结果 HlyAN436成功地在大肠杆菌表达系统表达，动物实验证实其具有良好的免疫原性和反应性以及一定的免疫保护性。

Based on the cloning FeSOD gene of the HZNH, the recombinant prokaryotic expression vector, PQESOa/FeSOD, was constructed and digested with restriction endonuclease BamH I and Pst I to check its construction. The PQE30a/FeSOD was then transformed into E.coli Ml5 and induced with IPTG. The high expression in vitro was obtained and analyzed on SDS-PAGE gel. The*results showed that the target proteins held a 37% portion in whole bacterial proteins and consisted of two parts, the soluble proteins and inclusion bodies. The soluble proteins in the aqueous layer, checked by means of activities of FeSOD enzymes and analyzed by means of activities of isozymogram from PAGE, demonstrated the induced expression proteins had the active nature of FeSOD enzymes.

以克隆的特异种质烟草HZNH的FeSOD基因为基础，构建了原核表达载体PQE30a／FeSOD，经限制性内切酶BamH Ⅰ、Pst Ⅰ双酶切鉴定后，再转化入大肠杆菌M15中，通过IPTG诱导，得到高效体外表达，经SDS-聚丙烯酰胺凝胶电泳检测，表达的目的蛋白占总菌体蛋白的37％，可溶性和包涵体两种形式均有存在，上清中的可溶性蛋白经FeSOD酶活测定和同工酶活性谱带分析，表明诱导表达的上清中的目的蛋白为有活性的FeSOD酶。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失