大肠杆菌

Antiviral 〔HBV, HCV and HIV〕, and anti-inflammatory 〔of Shigella dysenteriae, Bacillus anthracis, a, b-type melting Streptococcus pneumoniae, Staphylococcus aureus, E. coli, salmonella have both antibacterial antibacterial activity〕,〔Jiangtang glucose load can significantly lower blood glucose in mice, but also significantly against adrenaline-induced increase in blood glucose response in mice, and it can obviously confrontation Phenformin mice caused by hypoglycemia .

抗病毒〔 HBV ， HCV 与 HIV 〕，，抗炎〔对志贺氏痢疾杆菌，炭疽杆菌， a 、 b 型溶链球菌肺炎双球菌，金黄色球菌，大肠杆菌、沙门氏菌均有抗菌均有抗菌作用〕，降糖〔可使葡萄糖负荷的小鼠血糖明显降低，也能明显对抗肾上腺素引起的小鼠血糖升高反应，而且它还能明显对抗苯乙双胍引起的小鼠实验性低血糖。

The polysaccharide can inhibit the growth of Escherichia coli and Staphyloccocus aureus, and the scavenging effects of polysaccharide on the superoxide anion radical and nitroso were significant. It could be concluded that the polysaccharide displayed obvious bioactivities.

该多糖对埃希氏大肠杆菌和金黄色葡萄球菌均有抑制作用且对超氧阴离子自由基和亚硝基有较强的清除作用，说明该多糖具有明显的生物活性。

This molecule prevents a type of white blood cells, called macrophages, from killing internalised E.

分子阻止这一种白血细胞，称为巨噬细胞，杀死大肠杆菌内部化。

However, the study of genes related to mitochondrion is focused on E.coli, cattle and human, and there has no report on swine.

但是，目前对线粒体相关基因的研究集中于大肠杆菌、牛和人，在猪上还没有进行相关研究的报道。

Methods: One-step trizol had used to get the total RNA of the four animal brain, then PEGS000 to purify the small RNA from the total RNA, after the ligation ofpoly on the 3\' and the adaptor on the 5\', the oligo primer will be used to reverse translation the small RNA, and then used the specific primers to amplification the small RNA sequences. After the gel purition recovery, the small RNA will be ligated to the T-vector, and be transformed to the TOP 10 E. coli, at last, we picked up the masc. clones for sequencing.

通过Trizol一步法提取四种动物脑中的总RNA，接着用PEG纯化其中的小分子RNA，再经过poly连尾反应在小RNA的3'端连接上一段poly，然后在5'端连上adaptor，用Oligoprimer将连接好的小RNA进行反转录，用根据adaptor和Oligoprimer设计的引物来扩增小RNA，扩增后的小RNA进行胶回收后连接到T载体上，转化TOP10大肠杆菌后挑取阳性克隆进行测序，得到的序列再经过生物信息学的分析后确定是否为miRNA。

Each of the three RNA polymerases contains 12 or more subunits, some of which are similar to those of E. coli RNA polymerase.

原来译文：三种RNA聚合酶的每一种含有12个或更多亚基，其中有些亚基和大肠杆菌RNA聚合酶中的相似。

In order to study the expression, subcellular distribution and the function of NS2 protein, the coding region of NS2 was amplified from the hindgut tissue of cockroaches infected with PfDNV by RT-PCR and then the recombinant prokaryotic expression vector pET28a-NS2 was constructed. The recombinant plasmid was transformed into E. coli BL21 (DE3) to express the 6×His fusion protein in the bacteria.

为了对该基因进行深入的功能研究，从感染了黑胸大蠊浓核病毒的蟑螂的后肠组织中通过RT-PCR得到ns2基因编码序列，将其构建于原核表达载体pET-28a，转化大肠杆菌BL21(DE3)获得融合表达产物。

Objective To construct neisseria surface protein recombinants of Neisseria gonorrhoeae from a reference strain and express this protein in E.

目的 构建淋球菌标准株外膜蛋白NspA基因重组子，在大肠杆菌BL21 (DE3)中诱导表达外膜蛋白NspA。

Coli DH5α, the positive recombinants were identified with restriction enzyme analysis and DNA sequencing analysis.

将阳性重组子转入大肠杆菌DH5α，用IPTG诱导重组EBP融合蛋白的表达。

Analysis of the digestion of lysyl endopeptidase and N-terminal amino-acid sequence showed that Thr204 was the phosphorylated site which was consistent with the phosphorylation of Thr199 in DnaK.

赖氨酰肽链内切酶酶切、反相分离、氨基酸测序确定磷酸化位点为204位苏氨酸，与大肠杆菌Hsp70---DnaK的199位苏氨酸磷酸化一致。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失