大肠杆菌

However the Espf7 was gene engineering strain of DCase's genes cloned in E.coli.DCase expressed by Espf7 could transform NC-D-pHPG into D-pHPG.But DCase was in cell membrance,Dialysing cell membrance was investigated.

此外还有一菌种Espf7是克隆了DCase基因的大肠杆菌，它能表达DCase活性继续将NC-D-pHPG转化为D-pHPG，可DCase是胞内酶，故需要对其进行细胞破碎才能释放出酶。

However the Espf7 was gene engineering strain of DCases genes cloned in E.coli.DCase expressed by Espf7 could transform NC-D-pHPG into D-pHPG.But DCase was in cell membrance,Dialysing cell membrance was investigated.

此外还有一菌种Espf7是克隆了DCase基因的大肠杆菌，它能表达DCase活性继续将NC-D-pHPG转化为D-pHPG，可DCase是胞内酶，故需要对其进行细胞破碎才能释放出酶。

The fimbriae are the important virulence factors in enterotoxigenic E. coli. To detect and identify the four main fimbriae genes (K88, K99, F41, 987p) of ETEC from diarrhoeal piglets, a multiplex PCR assay was developed.

为了对猪源产肠毒素性大肠杆菌的4种主要菌毛（K88、K99、F41和987p）进行快速检测和分型，建立了检测4种菌毛的多重PCR方法。

PUC19 plasmid were successfully enriched and purified from the E. coli DH5α under the magnetic fields. The obtained plasmid retained native the bioactivity to be used for restriction enzyme digestions and cell transformations.

用这种方法成功地从大肠杆菌DH5α浓缩和纯化得到了pUC19质粒，该质粒具有生物活性，可直接用于限制性酶切和细胞转化等分子生物学下游操作。

METHODS: The rhBMP-2,expressed in Escherichia coli, was washed by Triton X- 100 and further purified by DEAE chromatography.The inclusion bodies were resolved in 8 mol/L urea, and were refolded and dimerized in the redox systems (reduced and oxidized glutathione). Finally, a one - step purification procedure based on the heparin affinity chromatography was implemented. The biological activity of purified rhBMP - 2 were tested by induction of the alkaline phosphatase activity in C2C12 cells.

在大肠杆菌中通过温度诱导表达重组人骨形成蛋白-2经过Triton X-100清洗之后，又通过DEAE离子交换层析纯化包涵体，包涵体在8 mol/L尿素变性溶解，在氧化-还原(还原型和氧化型谷胱甘肽)复性系统中，通过简单的稀释复性，通过肝素亲和层析一步纯化法纯化重组人骨形成蛋白-2，最后通过诱导C2C12细胞产生碱性磷酸酶检测重组人骨形成蛋白-2活性。

Harmless level was evaluated by Bacillus coli MPN and roundworm egg kill ratio.Digest doneness was determined by composting C/N and GI.

以堆肥的大肠杆菌最近似数和蛔虫卵杀灭率等评定堆肥的无害化程度。

Even the simplest life such as AIDS virus, colibacillus, eelworms and mosquitoes has a far more complicated structure than machine like automobile or TV does.

即使最简单的生命，如艾滋病毒，大肠杆菌、线虫、蚊子，它们的构造也是极复杂的，远远超过汽车、电视机等机器，但这些复杂体却能按DNA 中暗藏的指令，自下而上地建造起来。

The results showed that the three disparate treated substances have no visible antibacterial effect to E. coli , but a obvious effect to Staphylococcus aureus with inhibition of about 10 bacteriostasis units; have dissimilar effects to 10 kinds of fungi among the total kinds, and have a good inhibitory effect to Cytospora.coccodesHughes with fungistasis rate above 65%. After the gel filtration and activity detection, 50mM ammonium acetate was screened out as the more suitable eluents, the crudes were separated into strikingly unlike parts with obviously different antimicrobial activity.

结果表明，大蜡螟抗菌物质对大肠杆菌没有明显的抑制作用，而对金黄葡萄球菌抑制作用明显，抑菌活性单位在10左右；对10种植物病原真菌均有不同的抑菌活性，其中对苹果树皮腐烂病菌的抑制率达到65%以上；经过凝胶过滤分离，及对分离组分的抑菌活性测定，筛选出较合适的洗脱液为50mM乙酸铵，各分离组分抑菌活性差异明显，粗提物被分成了两个差异显著的部分。

In the medical field, using genetic engineering has successfully expressed many kinds of cytokines in protokaryon Bacillus coli, eukaryon yeast system and mammalian cells, which will make possibility of large-scale production of recombinant cytokines.

在医学领域，应用基因工程技术已在原核大肠杆菌、真核酵母和哺乳动物细胞成功表达了多种细胞因子，使重组细胞因子蛋白的大量生产和应用成为可能。

Are of four types in eukaryotes and only three types in E.

coli 在真核生物中有四种，而在大肠杆菌中只有三种

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。