大肠杆菌

API-B has been successfully expressed in both yeast and E. coli system, and the expression products have been purified to homogeneity. By the method of deletion mutagenesis, it was proved that the intact N- and C- terminuses of APIB were necessary for the inhibitory activity, consistent with the tertiary structures of the inhibitors from the STl superfamily.

成功地建立了API-B在酵母及大肠杆菌体系中的表达方法和纯化路线，并通过缺失突变的研究，证明API-B的N-端及C-端附近序列对于维持分子的高级结构和抑制活性非常重要，该结果与STI抑制剂超家族成员的结构特征相一致。

And starting tomorrow, the government will allow food producers to start zapping fresh spinach and iceberg lettuce with just enough radiation to kill E.coli and other dangerous germs.

从明天开始，政府将会允许食品生产者对菠菜和卷心莴苣使用适当的辐射来杀死E大肠杆菌和其他的一些危险的细菌。

The substance extracted showed some antibiotic activity against Staphylococcus aureus, Bacillus subtil, Escherichia coli, Aspergillus niger, penicillium, the minimum inhibitory concentration was 0.0625, 0.0625, 0.0313, 0.125, 0.25 mg/ml, and minimum bactericidal concentrations was 0.25, 0.125, 0.0313, 0.25, 0.5 mg/ml respectively.

该提取液对金色葡萄球菌、枯草杆菌、大肠杆菌、黑曲霉、青霉都有明显的抑菌效果，最低抑菌浓度分别为：0.062 5，0.062 5，0.031 3，0.15，0.25 mg/ml；最小杀菌浓度为0.25，0.125，0.031 3，0.5，0.25 mg/ml。

Homologous analysis of the deduced amino acid sequences were performed by BLAST and subsequently compared with GenBank data.

根据衣藻、矮牵牛花、Pisumsativum等真核生物hsp70a氨基酸高度保守序列设计两对简并引物，以热休克盐藻cDNA为模板进行巢式PCR扩增，产物经T-A克隆转化至JM109大肠杆菌中，经筛选后测序，将测序结果推导成氨基酸序列进行同源性分析，获得了盐藻热休克蛋白70a cDNA片段。

To test this hypothesis, we performed experiments using plasmids containing a luciferase reporter with amber, ochre and opal nonsense mutations within the luxB gene in Escherichia coli.

为验证此假设，我们用几个含有大肠杆菌荧光素酶报告基因的质粒进行了实验，这些质粒中的luxB基因分别包含有琥珀色、赭色和乳白色的无义突变。

In general as long as the temperature reaches 90 ℃, the common E. coli, hepatitis B virus and other bacteria have already perished, but we still continue to boil water, this area is to allow water in the calcium carbonate, magnesium carbonate and other ingredients to fully precipitate impurities, and the other On the one hand, because I am using tap water is treated with chlorine, so a certain degree of residual chlorine in water, in the process of boiling water, chlorine and impurities in the water will generate harmful substances called chloroform.

在一般的长期的温度达90℃，普通大肠杆菌，乙型肝炎病毒和其他细菌已经死亡，但我们仍烧开水，这方面是允许的碳酸钙，碳酸镁和其他水成分，杂质充分沉淀，以及对其他一方面，因为我使用的自来水是用氯处理，因此在水中的余氯，在一定程度上开水，氯和水中杂质的过程中，将生成所谓有害物质氯仿。

The aim is to get 3 green box at e..

其目的是让在大肠杆菌3绿箱。

The average kill rate against natural bacteria on kitchenware, such as table top, chopping block, knife, cupboard, porcelain bowl and the surface of refreshing box of the refrigerator were all above 0.972 0 when cleaning and polishing with vinegar solution for 10 min.

结果 不同浓度的食醋溶液作用不同时间对金黄色葡萄球菌、大肠杆菌和白色念珠菌的杀灭效果不同，浓度越大、作用时间越长杀菌效果越好（F=6.89～156.83，P.05）；对手自然菌作用2 min，平均杀灭率为0.991 5±0.003 2；对厨房内台面、砧板、菜刀、碗柜、瓷碗、冰箱保鲜室表面擦拭消毒10 min，对自然菌的平均杀灭率在0.972 0以上；对室内空气自然菌的杀灭率随熏蒸时间的延长而提高（F=121 2.13、502 4.46，P.05），当室内有人时，杀灭率有所降低（t=5.08～30.48，P.05）；置于54 ℃恒温箱（相对湿度≥0.75）存放14 d，醋酸总含量下降

Results Different concentrations of vinegar with different disinfection duration showed significantly different bactericidal effect on Staphylococcus, Escherichia and Candida albicans (F=6.89~156.83, P.05). The effect was better along with higher concentration and longer duration. The average kill rate against natural bacteria on hands was 0.9915±0.0032 after exposed to vinegar solution for 2 min. The average kill rate against natural bacteria on kitchenware, such as table top, chopping block, knife, cupboard, porcelain bowl and the surface of refreshing box of the refrigerator were all above 0.9720 when cleaning and polishing with vinegar solution for 10min. The bactericidal efficacy of vinegar against natural bacteria in room air was enhanced with fumigating duration prolonged (F=1212.13, 5024.46; P.05), while lowered when there were persons in it (t=5.08-30.48, P.05). The content of acetic acid in vinegar dropped by 8.372 g/L when it was stored at 54℃(relative humidity 0.75) for 14 days.

结果 不同浓度的食醋溶液作用不同时间对金黄色葡萄球菌、大肠杆菌和白色念珠菌的杀灭效果不同，浓度越大、作用时间越长杀菌效果越好(F=6.89~156.83，P.05)；对手自然菌作用2min，平均杀灭率为0.9915±0.0032；对厨房内台面、砧板、菜刀、碗柜、瓷碗、冰箱保鲜室表面擦拭消毒10min，对自然菌的平均杀灭率在0.9720以上；对室内空气自然菌的杀灭率随熏蒸时间的延长而提高(F=1212.13、5024.46，P.05)，当室内有人时，杀灭率有所降低(t=5.08~30.48，P.05)；置于54℃恒温箱（相对湿度≥0.75）存放14d，醋酸总含量下降

The reasons for using E.coli system to express HCV non-structure proteins are as follow:(1) Hepatitis C virus infections are serious in China. Using anti-HCV screening assay to control the quality of blood is the main measure of preventing the transmission of HCV.

本研究利用大肠杆菌系统表达丙型肝炎病毒非结构蛋白主要是基于以下考虑：(1)丙型肝炎在我国流行严重，利用HCV检测试剂控制血源质量是现阶段的主要预防措施。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。