大肠杆菌

YlyA sequence was amplified by PCR and cloned into pETMCSIII, to give pNG252. 6×His-YlyA was induced to express in BL21, overproduced YlyA was checked for its solubility and purified by Ni2+-NTA affinity chromatography. The result suggested that both the sequence and the direction of inserted fragment ylyA were correct.

PCR扩增ylyA序列，将其克隆到pETMCSIII中构建表达载体pNG252，用IPTG诱导6×His-YlyA融合蛋白在大肠杆菌BL21（DE3）中表达，对表达产物进行分析，最后对可溶性YlyA重组蛋白进行Ni2+-NTA亲和层析加以纯化。

Construction of E. coli transformants overproducing S1 and GDHTo express the carbonyl reductase S1 and GDH genes in the same E. coli cells, four expression vectors were constructed Fig.

为ㄋ在同─大肠杆菌细胞中表达碳酰还原酶S1和GDH基因，要构建四个表达型载体。

A California meat company recalled about 3500 pounds of hamburger patties that may be contaminated with E.

加利福尼亚州的一个肉类产品加工厂宣布回收大约3500磅汉堡肉饼，因为这些肉饼里可能带有大肠杆菌。

Amid a growing E. coli scare, Topps Meat Company is expanding its recall of frozen hamburger patties.

在对致病性大肠杆菌不断增长的恐慌中，Topps肉类公司扩大了召回冷冻汉堡小馅饼的数量。

Cephalexin is effective against a wide range of Gram positive and Gram negative bacteria including: Staphylococci, Streptococcus spp., Pneumococci, E. Coli, Klebslella spp, and Salmonella spp.

头孢氨苄是有效的针对范围广泛的革兰氏阳性和革兰氏阴性菌包括：金黄色葡萄球菌，链球菌属。，肺炎球菌，大肠杆菌，Klebslella菌和沙门氏菌。

In this research, B2 gene of HCV was highly expressed as a fusion protein (Trx-B2) by cloning into pThioHisA, and induced specific anti-HCV antibody in higher titer than unmodified B2 protein after immunize mice or rabbit.The results showed antigen Trx-B2 has obvious immunogenicity and induced specific antibody,which might be able to serve as diagnosis HCV. Consequently, this PcAb of rabbit was used to coated Immulon plate, and a method of both antibodys sandwiching antigens was developed to detect 32 HCV positive serum and 32 nomal serum.Results show that ratio of positive are 87.5%and 43.75% respectively.

但缺点是该抗原所诱导的抗体滴度较低，可能是由于其分子量较小，所以本课题将人工合成的HCV复合多表位抗原基因B_2克隆到表达载体pThioHisA中，利用硫氧还蛋白融合方式对该抗原进行修饰，并在大肠杆菌中表达融合蛋白(Trx-B_2)，纯化该蛋白后免疫小鼠及家兔，结果表明用硫氧还蛋白修饰后的融合蛋白免疫小鼠和家兔后，得到的抗体滴度均高于未经修饰过的复合多表位抗原合成肽所诱导的抗体滴度，并从免疫后的兔血清中提取IgG包被酶联板，利用双抗夹心法分别检测了32份HCV阳性血清和32份正常人的血清，结果阳性率分别为87.5%和43.75%，符合率为71.88%。

The observation on AFM indicated that the antibacterial mechanism of the antibacterial stainless steels should be attributed to leaking of the contents and shriveling of the cells in the bacteria .

原子力显微镜的观察表明，抗菌不锈钢对大肠杆菌的杀灭会表现为菌体内大量物质流失，细菌出现干瘪现象。

Results:The maximum of sterilizing rate was 64.90% and 91.57%,respectively.

结果：在经过5 min的循环冲洗后，酸性氧化电位水对花甲和黄瓜上大肠杆菌的去除率达最大值，分别为64.90%和91.57%。

Then the inhibition effects of the produced sulfated glucan on E. coli in vitro and in vivo were studied.

利用得到的硫酸化葡聚糖进行体外和体内抑制大肠杆菌效果研究。

DuPontQualicon also markets the award-winning BAX system, which usesleading-edge technology, including PCR assays, tableted reagents andoptimized media, to detect pathogens in food and the environment.

系统包括PCR系统，药片试剂和最佳的介质，能够检测到沙门氏菌，李斯特氏菌，大肠杆菌O157：H7，弯曲菌，金黄色葡萄球菌等其他致病菌。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。