大肠杆菌

The invention uses the fermentation liquor of one Escherichia coli generating polysialic acid as material, filters and removes thallus, adds some solvents as sodium chloride and alcohol into supernatant to deposit polysialic acid, dissolves the polysialic acid deposits via deionized water, filters to remove modified protein, uses alkali prolease to treat filtrate, complexes deposits via cetyl pyridinium chloride or cetyltrimethylammonium bromide, and dissociates, uses over alcohol to deposit polysialic acid, dissolves the deposits via ultra-pure water, grades and purifies via chromatography column, collects and dialyzes eluent, cools and dries to obtain polysialic acid.

本发明以一株产聚唾液酸大肠杆菌的发酵液为原料，经过过滤去除菌体，上清液加入适量氯化钠和乙醇等溶剂沉淀出聚唾液酸，聚唾液酸沉淀用去离子水溶解，再用过滤去除变性蛋白质，滤液经过碱性蛋白酶处理、氯代十六烷基吡啶或十六烷基三甲基溴化铵络合沉淀并解离后，再用过量乙醇沉淀出聚唾液酸，聚唾液酸沉淀用超纯水溶解，用层析柱进行分级纯化，收集洗脱液经过透析，冷冻干燥得聚唾液酸产品。

Extravasates from the microcapsules show good antibacterial effect on Staphylococcus and Hay Bacillus, but weak effect on Escherichia coli.

微胶囊渗出液对金黄色葡萄球菌、枯草杆菌有较好的抑制作用，对大肠杆菌的抑菌作用较弱。

The in vitro drug-release tests of the microcapsules were studied and the inhibition toward Staphylococcus, Hay Bacillus and Escherichia coli by their extravasates respectively were also performed via pervasion method.

方法是将头孢唑啉钠溶于海藻酸钠的PBS溶液，用正己烷／Span-85乳化形成微乳溶液，然后用CaCl2单凝聚固化得头孢唑啉钠的微胶囊；利用扩散法研究了该微胶囊渗出液对金黄色葡萄球菌、枯草杆菌和大肠杆菌的抑制作用。

Escherichia coli expressing F4 fimbriae is the major pathogenic bacteria that causes diarrhea in piglets before weaning.

大肠杆菌F4是引起仔猪断奶前腹泻的一种主要细菌，F4黏附于小肠上皮细胞是其致病的前提。

K88 fimbriae is one of the major colonization factors associated with porcine neonatal and post-weaning diarrhea caused by enterotoxigenic Escherichia coli.

K88菌毛介导产肠毒素性大肠杆菌在小肠上皮细胞的粘附，是引起新生仔猪腹泻的主要致病因子之一。

During the 29 days,no microorganisms could be detected in the pickled gingers sterilized by heating.

在29d的检测期间，杀菌保存的泡姜中没有分离到细菌、酵母菌、霉菌和大肠杆菌。

After heating sterilization, the content of Vc in pickled gingers was only 52.29% of the fresh gingers.

在杀菌保存的泡姜中，没有分离到细菌、酵母菌、霉菌和大肠杆菌。

Freeze-injured Escherichia coil at -20℃ can be recovered and the best recovery method is incubating it in NB for 2 h at 37℃.

-20℃冷冻大肠杆菌的损伤是可以修复的，在NB中37℃培养2h是其最佳修复条件。

Coli TG1 is selected for incubating and the antibody level is calculated after each screening.

产生的抗体的阳性克隆用大肠杆菌HB2151在IPTG的诱导下进行表达，提取出E。

It could be useful for the further study of NS1 gene function. Method The NS1 gene of H5N1 subtype AIV was amplified by RT_PCR and cloned to pGEM_T_easy vector. The NS1 gene expressing plasmids was constructed by inserting the target gene fragments into pGEX_4T_1 vectors. The expression proteins were indued by IPTG and analysed by Western blot.

方法采用RT_PCR方法对H5N1亚型AIVNS1基因进行扩增，将PCR产物克隆于pGEM_T_easy载体，将该基因插入pGEX_4T_1中构建NS1基因原核表达载体，转化BL21大肠杆菌后，在IPTG诱导下表达NS1蛋白，Westernblot鉴定表达NS1蛋白。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。