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An animal model of pulmonary lesions similar to clinical situation wasdeveloped by succussive intraperitoneal and intravenous injections of disintegratedE.coli in order toget a better model for adult respiratory distress syndromeresearch.

本文摹拟临床情况,先从腹腔然后再从静脉注射大肠杆菌死菌,以复制肺损伤的动物模型。结果发现,腹腔十静注者肺损伤比相同剂量单次静注者严重。

Several substrates of Lon protease have been known in Escherichia coli-including SulA and RcsA. However, the precise position of substrate-recognizing site in Lon protease is still not clear yet.

大肠杆菌中,有已知 Lon 的主要的基质为- SulA、RcsA,而Lon 蛋白酶本身辨识基质的确切位置却仍不甚了解。

However,when Sulfury fluride, Methy1 bromide and Chong-Jun-Wei were used in two drug combination separately, their insecticidal effect could be raised obviously.

结果:硫酰氟、溴甲烷和虫菌畏单一使用,在常用浓度下对大肠杆菌灭菌率在25%以下,对淡色库蚊24h杀灭率在30.13%以下;常用浓度加倍量对淡色库蚊24h杀灭率在55.20%以下;对德国小蠊和美洲大蠊的杀灭率亦仅为39.17%和43.33%以下。

This study is about the production of phage antibody with infection of help phage M13KO7 in a supE suppressor strain (TG1) of E.

本研究是有关噬菌体抗体在大肠杆菌supE抑制性菌株(TG1)中感染辅助噬菌体M13KO7的生产。

This paper analyses the problem of abnormal turbidity in the operation of a small water treatment plant in north Jiangsu,and holds that its main causes are contamination of raw water,abnormal water turbidity before filtration,filter trouble and superstandard of colibacilli.

对苏北某小型水厂运行过程中出现的浊度异常问题进行了分析,认为原水受到污染、滤前水浊度异常、滤池故障、大肠杆菌超标等是引发浊度异常的主要原因。

Prior studies have showed that pigs can be rendered immune to Ascaris suum infection by immunisation with crude antigen such as third stage larvae, and ES. Recently, some Escherichia coli-expressed recombinant antigen from A.

研究表明,猪蛔虫三期幼虫、分泌排泄物等天然抗原对宿主能提供有效的免疫保护,一些猪蛔虫融合大肠杆菌重组表达蛋白也可作为防控猪蛔虫病的候选疫苗。

Three expression vectors containing mutant gfp which were truncated from 3′ termine were constructed in the present paper.The mutation gfp with different length under the control by T 7 promoter were expressed in E. coli BL21 cells.

本文将突变型 gfp3′末端不同程度地截短,构建成表达载体,在大肠杆菌 BL 2 1中表达了不同长度的绿色荧光蛋白,当其生色基因中 65位 Ser点突变成 Thr,则 GFP的激发波由 396nm增至 4 70 nm,不用 UV激发,仅在自然光源下就可看到强烈的绿色荧光,发光强度增加 6倍,若从 3′端截短 12个氨基酸密码,对发光无影响,但截短 75个氨基酸密码,则激发波长降到 360 nm,发光强度减少 12倍。

Here we show, using single-molecule two- and three-colour fluorescence resonance energy transfer, that tetrameric SSB can spontaneously migrate along ssDNA.

允许SSB与ssDNA保持牢固结合,同时允许DNA接触其他蛋白的一个机制,该机制已在对大肠杆菌的这种蛋白所进行的一项单分子荧光共振能量转移研究中被发现。

Although thermostable lipases have great advantages, they are normally produced at low levels.

本课题亦采用此方法,在大肠杆菌中克隆并表达嗜热脂肪地芽孢杆菌脂肪酶基因。

Full-length plasmid DNA was amplified with the above primers with a high-fidelity thermostable DNA polymerase capable of long-rang amplification.

用能扩增长片段的高保真耐热 DNA聚合酶扩增全长的质粒DNA,直接转化大肠杆菌

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