大肠杆菌

Coli. The outer membrane protein were extracted from 38 strains of avian E. coli which were isolated from the dead chickens of chicken breeding farms in 3 areas of Baoding, Qiuhuangdao and Beijing by using ultrasonic cleaving and N-Lauroyl Sarcosine Sodium, and OMP typing was done by SDS-PAGE.

方法］对分离自保定、秦皇岛和北京3个地区规模化养鸡场的病死鸡体内的38株大肠杆菌，采用超声波裂解和N-十二烷基肌胺酸钠进行外膜蛋白提取，利用SDS-PAGE进行Omp分型。

The results showed that 0.01,0.05 and 0.10 g/ml extract of Saxifraga stolonifera Meerb had strong antimicrobial effect on S.aureus,but 0.25 and 0.50 g/ml extract had promotive effect on S.aureus.And the extract of Saxifraga stolonifera Meerb of different concentration had antimicrobial effect on E.coli.

结果表明，0.01、0.05、0.10g/ml 3种较低浓度的提取物对摇床培养的金黄色葡萄球菌生长具有较强的抑制作用，而0.25和0.50g/ml 2种较高浓度的提取物对摇床培养的金黄色葡萄球菌生长有较强的促进作用；这5种浓度的提取物均对摇床培养的大肠杆菌生长表现为抑制作用。

Objective To study the antibacterial function of seleniferous Lycium Barbarum on staphylococcus aureu and escherichia coli.

目的 探讨富硒枸杞对金黄色葡萄球菌和大肠杆菌的抑制作用。

Seleniferous Lycium Barbarum can effectively inhibite the activity of staphylococcus aureu and escherichia coli. With the increase of concentration, bacteriostatic effect increased.

富硒枸杞能明显抑制金黄色葡萄球菌和大肠杆菌活性，而且随着浓度的增加，抑菌效果逐渐增强。

Coli bacteria and seres as a model that scientists use to understand mobile DNA.

那项工作利用 Lamda 病毒来完成，Lamda 病毒可以感染大肠杆菌，它是科学家用来了解机动DNA 的一个模型。

Above all, Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli are the main pathogens causing bovine subclinical mastitis; the bovine clinical mastitis is usually caused by Streptococcus agalactiae. Antibiotics such as Enrofloxacin, Erythromycin and Kita sermonic show effective on treatment; the preparation of Chinese traditional medicine has great potential on bovine mastitis treatment and should be widely spread.

实验结果说明：引起奶牛隐性型乳房炎的主要致病菌类型为无乳链球菌、金黄色葡萄球菌和大肠杆菌，引起临床型乳房炎的主要致病菌是无乳链球菌；抗生素中的恩诺沙星、红霉素、庆大霉素用来治疗奶牛乳房炎效果较理想，中药制剂在治疗乳房炎上应用潜力很大，应大力推广。

The scFv formed inclusion body in E. Coli. 8 mol/L urea was used to solubilize the inclusion body, and the scFv was then purified in two steps , including Ni 2+ chelating affinity chromatography and gel filtration.

大肠杆菌表达的单链抗体包涵体，用8 mol/L尿素裂解，经过Ni离子螯合亲和层析和凝胶过滤两步纯化后，纯度达到97%以上，再通过透析复性除去尿素。

Canine Parvovirns VP2 gene was solubly expressed in E. coli.

犬细小病毒VP2基因在大肠杆菌中的获得了高效可溶性表达。

Objective: To solubly express the canine parvovirus VP2 gene efficiently in Bacillus coli, and lay a foundation for the preparation of monoclonal antibodies against VP2 protein.

目的：犬细小病毒VP2基因在大肠杆菌中高效可溶性表达，进而为其特异单克隆抗体的制备奠定基础。

Here, by introducing C-terminally 6xHis-tagged human Nap1 gene into Escherichia coli and exploiting the nickel-charged sepharose gel system to purify the human Nap1 protein, we show that the Nap1 enzyme function is preserved in human as in lower organisms such as bacteria and plants using succinamic acid as the substrate.

此研究中，我们将已在胺基末端接上6个组胺酸标记的Nap1基因表现在大肠杆菌中，并使用接合上镍原子的琼脂糖凝胶纯化出人类Nap1蛋白，再以丁醯胺酸作为受质，证明人类Nap1蛋白具有酵素活性。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。