大肠杆菌

Results: The results showed that both the wild type of recombinant MLCK and the ATP binding site mutagenesis of MLCK expressed in E.coli quantifiably with a large amount and exist as a soluble form.

结果：实验结果显示重组MLCK和MLCK/△ATP在大肠杆菌中以可溶的形式大量表达；经CaM-Sepharose 4B和Superose 6 HR纯化，SDS-PAGE鉴定得到单一的表达条带。

For the analysis of the non-kinase activity and the relationship between kinase activity and non-kinase activity, the recombinant full length MLCK was expressed and purified quantifiably with the E.coli expression system. The ATP binding site site-directed mutants of MLCK which lost the kinase activity were acquired.

为了更好地研究MLCK分子的非激酶作用以及激酶与非激酶两者的关系，本实验利用大肠杆菌表达系统大量表达并纯化出完整的MLCK分子，并对其ATP结合位点进行定点突变，获得无激酶活性的MLCK突变体。

To optimize the expression of recomb inant human prothymosinα in TG-1 induced by IPTG in 15L NBS fermento r.

在15 L的生物反应器内，对重组人胸腺素原α在大肠杆菌TG-1中经IPTG 诱导后的表达条件进行优化；应用NBS-MICROS 15 L.T。

Object In this study we framed the refusion gene antibacterial peptide[ Cecropin A(l ~ 8)- Magainin (1 - 12),CA(1 ~ 8)-MA(l ~12),CAM] vetor and expressed it in the Escherichia.coli. Thenpurified the recombinded peptide in the Intein Mediated Purificationwith an Affinity Chitin-binding Tag-IMPACT system.

目的 构建重组基因抗菌肽[Cecropin A(1～8)-Magainin(1～12)，CA(1～8)-MA(1～12)，CAM]质粒载体并在大肠杆菌(Escherichia.coli)中表达，应用几丁质自切割分离纯化系统(InteinMediated Purification with an Affinity Chitin-binding Tag，IMPACT)分离纯化抗菌肽，并测定其抗菌活性。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，将溶酶体的靶向信号肽KFERQ连接在RTA的羧基端；将构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受态大肠杆菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

To study the effect of specific electromagnetic wave on the growth of microorganism,this paper uses TDP and TDP without radiation board to irradiate growing vintage saccharomycete and E.

为了研究特定电磁波对微生物生长的影响，用TDP和取下辐射板的TDP照射正在生长中的酿酒酵母菌菌液和大肠杆菌菌液，并检测菌液的OD值。

The antimicrobial activities were determined with paper disc diffusion method. The result showed that of Sapindus mukorossi extracts only inhibited Candida albicans and no antimicrobial activities to Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Pityrosporum ovale and Propionibacterium acnes.

配制无患子溶液进行抗菌试验，以圆盘滤纸扩散法分析，结果显示甲、乙、丙、丁醇溶液样品，唯对白色念珠菌具抑制效果，而对另外5种菌种(大肠杆菌、金黄色葡萄球菌、绿脓杆菌、皮屑芽孢菌、痤疮杆菌)无明显抗菌效果。

Results The results showed that the material with the antibacterial activity in sapium root bark primarily was in the part of large polarity, but there was antibacterial activity in the material of the methanol elution with larger polarity; and the inhibitory components of the soluble material of ethyl acetate was complex and the different parts had the variable inhibition to different bacteria and in general these materials had a certain inhibition to Staphylococcus aureus and Escherichia coli.

结果］乌桕根抑菌活性物质主要存在极性较大的部分，但极性很大的甲醇洗脱部分也没有抑菌活性；乙酸乙酯可溶部抑菌成分较复杂，不同部分时不同细菌的抑制作用不同，总体来说对金黄色葡萄球菌和大肠杆菌有一定的抑制作用。

The result expressed that under the concentration of 0.2mg/mL, the 6 compounds are all active to Sarcina lutea, ME-1, MT-6, MT-land MT-7 are active to Bacillus megaterium, to Escherichia coli, only MT-2 is active. The dimeric natural products are intensively existed in botany, most of them have the obvious biological activities. They have been concentrated on in recent years, and they are one of the compounds to which are enjoyed great popularity in natural product.

结果表明，在浓度为0.2mg/mL的条件下，六种化合物对藤黄八叠球菌均具有明显抑制作用，ME-1、MT-6、MT-1、MT-7对巨大芽孢杆菌有抑制作用，MT-2对大肠杆菌有抑制作用，对绿脓杆菌和枯草芽孢杆菌六种成分均无抑制作用；二聚体化合物在植物界广泛存在，多数二聚体天然产物具有显著的生物活性，引起人们的重视，是天然产物化学界研究的热门化合物之一。

Additionally, 75 volatile chemical constituents have been identified from the petroleum ether extracted part by capillary GC-MS, and the relative percentages of them are determined by GC method. The result showed that the volatile constituents of Musella lasiocarpa mainly includes advanced alkane, aliphatic acid and ester, aldehyde, ketone, aromatic and its heterocyclic compounds. With the compounds that have been isolated and identified from Musella lasiocarpa, the biogenensis pathway of new compound MT-3 is approached. Furthermore, the bacteriostasis of the 6 compounds to Escherichia coil, Pseudomonas aerusinosa, Bacillus megaterium, Sarcina lutea and Bacillus subtilic are determined by flat dilution method.

此外，从其石油醚萃取部分用毛细管色谱法分析鉴定出75种挥发性化学成分，并测定了各组分的相对百分含量，结果表明，地涌金莲挥发性化学成分以高级烷烃、高级脂肪酸酯及醛、酮、芳香和芳香杂环化合物为主；根据从地涌金莲中鉴出和分离得到的化合物，探讨了新结构化合物MT-3的生源代谢途径；采用平板稀释法测定了地涌金莲中六种化学成分对大肠杆菌、绿脓杆菌、巨大芽孢杆菌、藤黄八叠球菌和枯草芽孢杆菌的抑菌作用。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。