大肠杆菌

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion.

应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石。。。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

中文题名斜带石斑鱼生长激素分泌及其基因表达调控的研究副题名外文题名 Regulation of growth hormone and its gene expression in orange-spotted grouper，Epinephelus coioides 论文作者冉雪琴导师林浩然学科专业水生生物学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数140页关键词生长激素基因表达石斑鱼馆藏号BSLW /2003 /Q959 /39 应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

7Kb of these isolates were amplified by using RT-PCR The amplified DNA were then cloned and sequenced The analysis of the sequence data further confirmed that IBV was the pathogenic agent of the infectious disease associated with proventricular lesion

利用扩增片段两端的EcoRⅠ和BamHⅠ位点将扩增片段克隆进pBluescripⅡKS载体，转化大肠杆菌JM109，挑选白色菌落并用PCR方法对插入片段进行鉴定。用双脱氧终止法对扩增的片段进行测序并与GenBank公开的序列进行比较分析。结果进一步证实IBV是导致此种野外发生的腺胃相关疾病的病原。

Objective To construct an expression vector for fusion gene of heat-liable enterotoxin B subunit and major outer membrane protein of Chlamydia psittaci and express the fusion gene in prokaryotic cells.

目的构建含大肠杆菌热不稳定肠毒素B亚单位和鹦鹉热衣原体主要外膜蛋白融合基因的表达载体，并在原核细胞中表达融合蛋白。

Chlamydia psittaci ; Escherichia coli heat-liable enterotoxin B subunit ; Major outer membrane protein ; Prokaryotic expression

鹦鹉热衣原体；大肠杆菌热不稳定肠毒素B亚单位；主要外膜蛋白；原核表达

The inhibitory tests in vitro were performed against Escherichia coli, Salmonella pullorum with filter paper method and tube dilution method. And the diameters of inhibition zone, minimal inhibitory concentrations of levofloxacin combined with polymyxin E were measured.

方法］应用纸片法和试管稀释法进行体外抑菌试验，测定左氧氟沙星与多黏菌素联合对鸡大肠杆菌、鸡白痢沙门氏菌的抑菌圈大小和最小抑菌浓度。

The S. pullorum on the surface of shell eggs was completely killed and E. coli O157:H7 logarithm of counts decreased to less than 1.0 log10 by NEW containing 12 mg/L available chlorine for 3 min without viable cells in the washing water after treatment.

当中性电解水清洗消毒被人工污染的鸡蛋表面时，有效氯质量浓度为12 mg/L、处理3 min可将鸡蛋表面的鸡白痢沙门氏菌全部杀灭，大肠杆菌O157：H7菌落数对数降低到1.0 log10 以下，且处理废液中没有残存菌，无二次污染问题。

For the sake of developing an effective, safe and no pollution disinfectant to reduce or eliminate food-borne pathogens on shell eggs, the bactericidal activity of neutral electrolyzed water (NEW, pH 6.0-7.5) generated by electrolysis of diluted hydrochloric acid in a non-membrane electrolytic cell for inactivation of Salmonella pullorum, Escherichia coli O157:H7 and artificial inoculated shell eggs initial logarithm of population of two pathogens on the eggs were approximately 6.19-6.26 log10 (cfu/g and 6.12-6.19 log10 respectively was evaluated.

为寻求一种高效、安全、无污染的禽蛋清洗消毒剂，采用无隔膜电解装置电解稀盐酸溶液制备中性电解水（pH值6.0～7.5）考查不同有效氯浓度、处理时间和温度条件下中性电解水对鸡蛋人工接种鸡白痢沙门氏菌（Salmonella pullorum，鸡蛋表面的初始菌落数对数为6.19～6.26 log10）和大肠杆菌O157：H7（鸡蛋表面的初始菌落数对数为6.12～6.19 log10）的杀灭效果。

Coli lpdA mutant has the potential to produce pyruvic acid from xylose and mannose.

这些结果对构建以大肠杆菌为母体的模式&细胞工厂&有参考价值。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。