大肠杆菌

In this study, the effects of culture conditions on the growth of recombinant E. coli BL21 (DE3) and the expression of subunit protein of Pasteurella multocida toxin 1 (Tox1) were investigated in both shaking flask and fermentor.

摘要 本研究探讨携带有猪萎缩性鼻炎次单位毒素Tox1基因之重组大肠杆菌大量表现重组蛋白质的最适条件，并对Tox1进行部份纯化。

This method could amplify the specific fragment in the reference strains, but not in chicken E. coli, Haemophilus paragallinarum, Salmonella Pullorum and Avian pasteurella multocida, of ORT. A sensitivity test indicated that the assay's lowest detection limit was 90 pg DNA.

该方法能在ORT参考菌株中扩增到特异性片段，而鸡大肠杆菌、副鸡嗜血杆菌、鸡白痢沙门氏菌、禽多杀性巴氏杆菌的扩增结果均为阴性；敏感性试验表明该方法最低检出限量为90 pg DNA。

Secondly, the rate of infection of the Pasteurella multocida reached to 16%(12/75), Riemerella anatipestifer bacilliis also high, the ratio was 12%(9 / 75). More over, hemolytic bus bacteria, Salmonella and Staphylococcus aureus were also showed in the poultry tested.

结果表明：大肠杆菌仍是该部分地区危害家禽的主要致病菌，它的个体感染率高达41.3%（31/75）；其次是多杀性巴氏杆菌，个体感染率为16%(12/75)；鸭疫里默氏杆菌的个体感染率也较高，为12%（9/75）；此外还分离到溶血性巴氏杆菌、沙门氏菌和葡萄球菌。

In addition, Mycoplasma bovis, E. coli, Salmonella, Streptococcus, Staphylococcus and Aspergillus fumigatus was successfully isolated from the lungs and other tissues, however, no Pasteurella was isolated.

从肺等组织中分离到牛支原体以及大肠杆菌、沙门氏菌、链球菌、葡萄球菌和烟曲霉，没有分离到巴氏杆菌。

Coli. The antimicrobial activity of the recombinant fusion protein was evaluated in vitro, which exhibited high antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, and Pasteurella multocida, but low antimicrobial activity against Escherichia coil, and no antimicrobial activity against Salmonell choleraesuis.

对该重组蛋白进行纯化与体外抗菌活性的测定。结果表明，重组鸡AvBD6融合蛋白对多杀性巴氏杆菌、金黄色葡萄球菌、枯草芽胞杆菌有较高抗菌活性，对大肠杆菌的抗菌活性较弱，对猪霍乱沙门氏菌无抗菌活性。

Specific pathogen free chicken ; Escherichia coli ; mildly pathogenic avian influenza virus ; synergistic pathopoiesis effects ; immunosuppression

SPF鸡；大肠杆菌；低致病性禽流感病毒；协同致病；免疫抑制

Tuberculosis H37Rv strain was amplified by PCR and cloned to vector pEASY T1. After sequencing, it was subcloned to fusion expression vector pET28a and then transfected to E coli BL21 (DE3). It was found that the LprG protein was successfully expressed after induction with LPTG.

采用聚合酶链反应从结核分枝杆菌H37Rv基因组中扩增出LprG基因，克隆到pEASY T1中，序列测定正确后，再亚克隆到融合表达载体PET28a中，转化入大肠杆菌BL21(DE3)，经IPTG诱导表达。

At the same time,the signal peptide secreted sweet protein monellin from periplasm to the medium too.

同时，用该信号肽序列还实现了甜蛋白monellin基因在大肠杆菌中的分泌表达。

When protein folding is compromised in the periplasm of E.

当大肠杆菌E.coli周质中蛋白折叠发生阻碍，外膜蛋白OMPs的C端与三聚体DegS蛋白酶的 PDZ区域发生结合，促使ResA裂解。

The results showed that the three scFvs specific for Xac were successfully expressed in E. coli HB2151. The molecular weight of expressed products was about 32.0×103 and the products were mainly located in the periplasm.

结果显示，3株抗柑桔溃疡病菌可溶性单链抗体在大肠杆菌HB2151中均获得成功表达，表达产物分子量约为32.0×10^3，主要集中于细菌周质腔中。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。