大肠杆菌

Schistosoma japonica antigen cDNA clones were identified by lysogenic expression,flat lyiic method and PCR amplification. All 8 positive clones immunologically screened could be expressed in E- coli in the form of fusion proteins with the molecular weight being about 140 to 150 kDa. The positive cDNA genes were digested by restriction endonuclease EcoRI,then the agarose gel electrophoresis revealed the size of them being 700 to 900 bp.

利用融源表达、平板裂解法和PCR扩增三种不同方法分别对日本血吸虫抗原cDNA基因进行鉴定和分析，8个免疫筛选阳性克隆均能在大肠杆菌中以融合蛋白的形式表达，表达蛋白分子量为140～150kDa，抗原cDNA基因经限制性内切酶EcoRI酶解后，琼脂糖凝胶电泳显示其大小为700～900bp，PCR能扩增出特异性条带。

Results We obtained a lysogenic bacteriophage MZTP01with clear plaque and 1 mm diameter. Fragment D with 2362bp (Genebank No. AY639599) was obtained after the phage DNA hydrolyzed by HindⅢ/EcoRⅠ. Among fragment D, the pep gene with molecular weight of 47 kDa and length of 1101bp was cloned and expressed. Recombinant M15 (pQE30pep) was built and overexpressed in Escherichia coli with a 47kDa clear band. At the same place a clear band was observed by Western blot. Judging from the time course expression, we could conclude that PEP protein produced at 1 hour after induction and then increased gradually. PEP protein was mainly in the form of inclusion body in the recombinant and slowed the growth speed of host. Homologous comparison of PEP protein from phage MZTP01 with other PEPs from BLAST were that phage MZTP01 PEP protein had 100% homologe with that of Escherichia coli K12, and most of others took the similarity in the range between 37%~84%.

诱导获得的溶原性噬菌体MZTP01斑点清晰，直径约1mm，成斑时间12h；从噬菌体基因组DNA双酶切(HindⅢ/EcoRⅠ)片段中回收长度为2362bp的D片段(Genbank登录号： AY639599)，又从D片段中克隆了长度为1101bp、编码367aa、分子量为47kDa的pep基因，表达载体M15(pQE30pep)在大肠杆菌(Escherichia coli， E.coli)中表达获得了47kDa的清晰表达带，在1h 时开始产生蛋白并有逐步上升的趋势； Western blot 也在47kDa处得到一条清晰的条带；可溶性分析表明PEP蛋白在重组菌株中是以不可溶的包含体形式存在的，该蛋白的产生明显地抑制了宿主的生长速度；噬菌体PEP氨基酸序列之间的同源性比较表明，噬菌体MZTP01 PEP蛋白与来自E.coli K12噬菌体的PEP蛋白的同源性程度最大。

Bacteriostasic activity experiments on anthocyanin extracted from Malva sylvestris inhibiting Staphylococcus aureus, Escherichia coli, Aspergillus niger, were conducted by using solid and liquid culture methods.

本项研究通过采用固体培养和液体培养的试验方法，进行大花葵花色苷对金黄色葡萄球菌、大肠杆菌和黑曲霉的抑菌试验。

Methods use monoclonal antibody against mammiferous hsp70 to recognize the related antigens of Bacillus pyocyaneus,Bacillus proteus,Bacillus coli,Staphylococcus aureus and Klebsiella pneumoniae with modified western blotting.

用抗哺乳类热休克蛋白（heat shock protein，hsp）70抗体识别绿脓杆菌、变形杆菌、大肠杆菌、金黄色葡萄球菌和肺炎克雷白杆菌抗原，进行免疫转印分析，测试正常豚鼠膜迷路中hsp70的表达水平。

Firstly, microbiologic incubation and detection had been carried out to understand the fundamental biologic characters of E. coli O157:H7 and to elementarily identify it by using SMAC culture medium.

首先对其进行微生物学培养和检测，熟悉大肠杆菌O157：H7的基本生物学特性，并掌握用山梨醇麦康凯培养基初步鉴定O157：H7的方法。

The rate of clinical success, defined as cured plus improved patients, was 75% for levofloxacin and 72.8% for ciprofloxacin (95% confidence interval for the difference in the success rates,-13.27 to 8.87). Microbiologic eradication rates were also similar in both groups (75% for levofloxacin and 76.8% for ciprofloxacin; 95% CI for the difference,-8.98 to 12.58). The most common isolates were Enterococcus faecalis and E. coli . Both regimens were well tolerated, with similar rates of adverse events and of relapse by six months.

治疗成功的定义为患者的病情痊愈并且症状获得改善，结果接受levofloxacin治疗的患者成功率为75%，而接受ciprofloxacin治疗的患者则为72.8%（信赖区间为-13.27 ~ 8.87）；两组的微生物根除率相当（levofloxacin组为75%，ciprofloxacin组为76.8%；信赖区间为-8.98 ~ 12.58）；最常被分离出的病菌为乳酸球菌与大肠杆菌，这两种药物的耐受性都很好，副作用与六个月的复发率也都相当。

METHODS:The way of inhibition of Escherichia coli,Bacillus pyocyaneus,Staphylococcus aureus by amicacine and gintamincine,the methods of the plate,the tube and U-type disk (quantitative analysis by microdose and germ MQQA) were compared in nathre.RESULTS:Minimun inhibitory concentration by MQQA was lower than that by other two methods with higher sensitivity.

以大肠杆菌、绿脓杆菌和金葡球菌为实验对象，以硫酸丁胺卡那霉素、庆大霉素2个已知药为工具药，用MQQA测出2个已知药对上述3个菌株的最小抑菌浓度，并与平皿法和试管法进行比较。

Resultsbacteriostatic test in vitro express: it is effective for both of bacterium and fungus (red trichophyton, purple trichophyton, trichophyton mentagrophytes, microsporum gypsen).

结果体外抑菌试验结果表明，该药对细菌(金黄色葡萄球菌、大肠杆菌)和真菌(红色毛癣菌、紫色毛癣菌、须毛癣菌、石膏样小孢子菌)均有抑制作用。

Abstract］objectiveto observe antiseptic effect and bacteriostatic concentration in vitro of tieshanxuanyangping.methodsto determine bacteriostatic concentration in vitro for staphylococcus, bacillus coli, red trichophyton, purple trichophyton, trichophyton mentagrophytes, microsporum gypsen with doubling dilution.

摘要］目的观察&铁扇癣痒平&体外抗菌效果和抑菌浓度。方法采用对倍液体稀释法测定该药对金黄色葡萄球菌、大肠杆菌、红色毛癣菌、紫色毛癣菌、须毛癣菌和石膏样小孢子菌体外抑制作用。

MethodsTo determine bacteriostatic concentration in vitro for staphylococcus, bacillus coli, red trichophyton, purple trichophyton, trichophyton mentagrophytes, microsporum gypsen with doubling dilution.

方法采用对倍液体稀释法测定该药对金黄色葡萄球菌、大肠杆菌、红色毛癣菌、紫色毛癣菌、须毛癣菌和石膏样小孢子菌体外抑制作用。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。