大肠杆菌

The proteolytic domain has a unique fold and assembles into hexameric rings, and the substrate recognition site locates in the N domain.

虽然在本研究中，是使用大肠杆菌 K12 作为研究的材料。

Then the fusion protein was primary purified by affinity chromatography. The GST tail of the fusion protein was cleaved by Thrombin. Nucleotide sequence analysis showed that the gdh gene of S.suis possesses the highly conserved motifs typical of family I hexameric GDHs.

猪链球菌2型谷氨酸脱氢酶编码基因克隆、序列分析及原核表达根据已发表的猪链球菌2型的gdh基因设计引物，扩增海安病人分离株Habb中的目的基因，并进行序列测定和分析；构建重组表达载体pGEX4T-2-gdh，在大肠杆菌中表达，并纯化重组蛋白。

All countries scholar have been looking for new cavitation to replace the ultrasonic cavitation technique, the hydrodynamical technique have lower cost and equipment simpler characteristics than the light or particle cavitation , so it was valuable of

本文所做的主要工作及所取得结论： 1 设计并组装了一套水动力空化反应循环系统； 2 设计了3种不同型号(孔径分别为1毫米、2毫米、4毫米)的多孔板； 3 比较了不同板在不同条件下的空化效应强弱； 4 利用该装置对大肠杆菌菌液进行消毒杀菌的实验研究；结论：(1)在孔径较小时，空化效应更强

Secondly, although protein sequence hydropathy prediction result showed ZmDWF1 is an integrated membrane protein, we still successfully expressed the full-length protein fused into the plasmid pET30a , then purified the 70 KD fusion proteins by Ni-NTA agarose affinity chromatography column.

其次，尽管蛋白氨基酸序列的疏水性分析表明，ZmDWF1是一种整合膜蛋白，我们依然将ZmDWF1全长序列克隆到原核表达载体pET30a中，并成功的在大肠杆菌BL21（DE3）中表达了这种预测的膜蛋白全长。

The Escherichia coli is the most common pathogenic bacilli both on the medicineand the clinical veterinarian, the pathogenic can cause young pig"s yellow diarrheaand white diarrhea,and pig"s hydropsy, and so on.

大肠杆菌是医学和兽医临床上最常见的一种病原菌，其致病性株可引起仔猪黄痢、白痢、猪水肿等病。

The N gene ORF was then subcloned into pET-30a vector and the recombinant plasmid was transformed into E.coli BL21 (DE3) and induced with IPTG. The protein expression was determined by SDS-PAGE. The expressed protein had a molecular weight of 54.4 Ku that existed as inclusion body. Thin-layer scanning showed that the expression product accounted for 30.5% of the total bacterial proteins. The recombinant protein possessed native biological activity and could react with anti-PEDV hyperimmune serum in Western blot.

以阳性质粒为模板，用分别含有BamH Ⅰ和Xho Ⅰ酶切位点的上、下游引物扩增得到ORF，其PCR产物经BamH Ⅰ和Xho Ⅰ双酶切后定向克隆到pET-30a载体，构建的重组质粒命名为pET-30a-PN；将pET-30a-PN转化到大肠杆菌BL21 (DF3)中，在IPTG诱导下进行表达；SDS-PAGE结果表明表达出与预期大小相符的约54.4 Ku的重组蛋白，重组蛋白以包涵体形式存在；薄层扫描结果表明表达产物占菌体总蛋白的30.5%；Western blot分析表明表达的重组蛋白能与抗PEDV高免血清反应，说明该重组蛋白具有免疫学活性。

In this study, we refer to the NCBI database to design primers which are used for amplification of the gene fragment of hepatopancreatic parvovirus、infectious hypodermal and hematopoietic necrosis virus and taura syndrome virus.

本研究从NCBI database资料中，分别在肝胰小病毒(hepatopancreatic parvovirus， HPV)、传染性皮下及造血组织坏死病毒(infectious hypodermal and hematopoietic necrosis virus， IHHNV)和桃拉病毒(taura syndrome virus， TSV)等三种常见之虾病毒中，各选出一段壳蛋白基因，利用重组蛋白的方式，在大肠杆菌中大量表现。

The recombinant immunotoxin BDI-PE38 was expressed mainly in soluble form and can be purified by Ni(superscript 2+)-NTA agarose.

在基因工程的实践中，包含体的形成是人们利用大肠杆菌表达外源蛋白的一大难题。

We also found the time when the glucose was added into the medium greatly affected the expression level of the recombinant immunotoxin.

本文报导了利用山梨醇、甜菜碱等相容性溶质，在高盐胁迫下，实现了重组免疫毒素在大肠杆菌周质腔中的可溶性表达。

Day-old specific pathogen free chickens were inoculated intratracheally with lowly virulent E.coli and /or mildly pathogenic avian influenza virus.

SPF鸡经不同顺序、不同时间间隔人工感染MPAIV和E.coli173株(O4)后，对试验鸡的病死率和针对大肠杆菌不同抗原体液免疫应答水平等进行研究。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。