大肠杆菌

Staphylococcus aureus, Streptococcus agalactiae, Bacillus cereus, Escherichia coli, Salmonella choleraesuis, Salmonella enteritidis and Salmonella typimurium were used as tested bacteria, the bacteriostasis was studied and the minimal inhibitory concentration was measured.

以金黄色葡萄球菌、无乳链球菌、蜡样芽孢杆菌、大肠杆菌、猪霍乱沙门氏菌、肠炎沙门氏菌及鼠伤寒沙门氏菌为供试菌，研究其抑菌活性，并测定其相应最小抑菌浓度。

Edema disease is a common cause of illness and death loss in pigs during the first 2 weeks after weaning. The disease is an enterotoxemia caused by Shiga-like toxin Escherichia coli that colonize the small intestine and produce SLT-IIe.

猪水肿病(Porcine Edema disease，ED)是仔猪常见的一种急性致死性传染病，主要由某些特定血清型产类志贺毒素大肠杆菌(Shiga-like toxin Escherichia coli，SLTEC)引起的肠毒血症。

As an immunomodulator NO may play a role like a double-edged sword since it expresses both in immuonchalleged and immunosspresed states. 2. After received external stimulus the morphology of ependyma of SFO could be changed and varied with time course, particularly its cellular apoptosis do occur and earlier than the ordinary areas of the brain. 3. Astrocytes in SFO could express cFos protein induced by LPS, suggesting the cells may maintain homeostasis in the brain in a longer period and modulate immune state of the brain. 4. As a CVO, SFO may sense changes of blood-bearing immunosignals earlier and instantly response to them, thus as one of windows of the brain SFO do take part in immunomodulation within the brain.

通过我们的实验结果我们可以得出这样一些初步的结论：1、一氧化氮作为免疫调质参与了机体免疫，它既能在免疫激发状态下又可在免疫抑制状态下进行表达提示，NO在脑内免疫调节中的作用可能是双向的。2、穹窿下器的室管膜形态在接受外界刺激后，随着时间的变化而不同，扫描电镜下的凋亡形态虽需进一步证实，但室管膜细胞确实发生了凋亡，并且穹窿下器发生的较早。3、穹窿下器内的星形胶质细胞在给予大肠杆菌后确实可以表达c-fos蛋白，并可能通过自身的激活来较长时间的维持体液的动态平衡，调节脑内免疫状态。4、穹窿下器作为感受性室周器官之一，能够较早的感受血携免疫信息的改变并及时作出反应，可能作为外周免疫信息进入脑内的窗口之一来参与脑的免疫调节。

In the present study changes of NOS positive cells after injection of LPS and apoptotic ependymal cells, the relationship between expression of immediate early gene c-fos and astrocytes, and comparison of changes between SFO and rest regions of the brain in different time-points were investigated with NADPH-d histochemistry, apoptosis in SITU detection of TUNEL, TEM and SEM, fluorescein double labeling of immunohistochemistry, and laser scanning confocal microscope .

为了证实穹窿下器在脑内免疫调节系统中的作用，在本研究当中，应用了NADPH-d的组化方法、TUNEL末端标记法、扫描及透射电镜方法、免疫组织化学的荧光双标法及激光扫描共聚焦显微镜技术，来研究在腹腔内给予大肠杆菌内毒素后，脑内的一氧化氮合酶细胞的变化、室管膜细胞的凋亡、早期即刻基因c-fos的表达与星形胶质细胞之间的关系，以及穹窿下器与其它脑区相比较时的特异性改变。

In the present study changes of NOS positive cells after injection of LPS and apoptotic ependymal cells, the relationship between expression of immediate early gene c-fos and astrocytes, and comparison of changes between SFO and rest regions of the brain in different time-points were investigated with NADPH-d histochemistry, apoptosis in SITU detection of TUNEL, TEM and SEM, fluorescein double labeling of immunohistochemistry, and laser scanning confocal microscope.

为了证实穹窿下器在脑内免疫调节系统中的作用，在本研究当中，应用了NADPH刁的组化方法、TUNEL末端标记法、扫描及透射电镜方法、免疫组织化学的荧光双标法及激光扫描共聚焦显微镜技术，来研究在腹腔内给予大肠杆菌内毒素后，脑内的一氧化氮合酶细胞的变化、室管膜细胞的凋亡、早期即刻基因（immediate early genes沁－fos的表达与星形胶质细胞之间的关系，以及穹窿下器与其它脑区相比较时的特异性改变。

Coli, Shigella sonnei s, Proteus, typhoid and vice Salmonella typhi, Pseudomonas aeruginosa, Vibrio cholerae and other enteric pathogens are effective; its ethanol extract on the number of gram-positive and-negative bacteria, and human-type Mycobacterium significant antibacterial activity; ebony decoction on in vitro to be sore dermatophytes, Epidermophyton floccosum, plaster-like pathogenic microorganisms in the skin of small spores of fungi

广谱抗菌：实验证明，乌梅水煎液对炭疽杆菌、白喉和类白喉杆菌、葡萄球菌、肺炎球菌等皆有抑制作用，对大肠杆菌、宋内氏痢疾杆菌、变形杆菌、伤寒和副伤寒杆菌、绿脓杆菌、霍乱弧菌等肠内致病菌也有效；其乙醇浸液对一些革兰阳性和阴性细菌，及人型结核杆菌均有显著抗菌作用；乌梅水煎液在试管内对须疮癣菌、絮状表皮癣菌、石膏样小芽孢菌等致病皮肤真菌有抑制作用

Methods The SpaA was purified by electroelution from NaOH-extracted antigen of Erysipelothrix rhusiopathiae strain C43311. The rSpaA-N was expressed in E. coli BL21 as a soluble protein by IPTG inducing,and purified with GST affinity chromatography. Mice of each group were subcutaneously immunized three times with 50μg or 100 μg of native SpaA, rSpaA-N or NaOH-extracted antigen with in complete or incomplete Freund adjuvant at 2-week intervals.

将猪丹毒丝菌C43311株SpaA-N以可溶形式表达在大肠杆菌BL21中，用GST Bind Resin纯化试剂盒纯化rSpaA-N，采用电洗脱法从猪丹毒丝菌C43311株NaOH提取抗原中纯化天然SpaA，将rSpaA-N、天然SpaA和NaOH提取抗原制成亚单位疫苗，同时设GST及生理盐水对照组，间隔2周分3次皮下免疫小鼠，第3次免疫后2周用100LD50猪丹毒丝菌C43065株进行腹腔攻毒，采用间接ELISA方法检测免疫组小鼠血清的抗体动态变化。

Adopting duple diluent method and stiletto method and external antibacterial test on escherichia coli, solmonella paratyhpi , staphylicoccus aureus, streptococcus,, pseudomonas aeruginosa , Erysipelothrix rhusiopathiae, pasteurellosis to get the MIC and MBC, we studied antibacterial effect of the Copmounds Solatium Iyratum Thunb injection.

本研究采用试管二倍稀释法和打孔法对大肠杆菌、沙门氏菌、金黄色葡萄球菌、链球菌、绿脓杆菌、丹毒杆菌、巴氏杆菌的最小抑菌浓度和最小杀菌浓度及体外抑菌试验，研究复方白毛藤注射液抗菌作用。

The jejunal lactase activity and erythrocytic GRd activity were significantly increased in L group.

结果发现，低剂量组粪便中大肠杆菌菌数显著降低，空肠黏膜lactase活性则显著提高，而且红血球GRd活性也显著增加。

Objective: To develop the best method to recover the freeze-injured Escherichia coil at -20℃.

目的：建立-20℃冷冻损伤大肠杆菌的最佳修复方法。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。