大肠杆菌

Firstly, the recombinant engineering technology related vaccine preparation was set up, including: 1. the gene knock-out based on linear DNA target molecular in vivo; 2. the selection and counterselection technology of exogenous gene knock in definite site on chromosome; 3. the Gap-repair clone technology in vivo; 4. the recombination technology of single-chain DNA and the overlapping primer; 5. Set up the new selection and counterselection technology of neo-E.

本研究首先建立与疫苗制备相关的重组工程技术包括：①基于线型DNA打靶分子的大肠杆菌体内基因剔除技术，②选择与反选择外源基因定位敲入染色体上特定位点技术，③Gap-repair体内克隆技术，④单链DNA重组技术和重叠引物介导的DNA重组技术，⑤建立&抗生素-酶切位点&选择反选择技术新技术。

In order to exploit the resources ofCrocus sativus and crocin further, molecular biological research of saffron wasstudied in the thesis.

为了研究西红花药用成分西红花甙的资源，更好地利用西红花甙，本论文对西红花进行了分子生物学方面的研究：对西红花酸合成酶(7，8-二加氧酶)基因的克隆，并将其在大肠杆菌中表达，纯化诱导蛋白，制备相应的特异性抗体。

Escherichia coli O157:H7 was cultured in sterilized fresh water at 4℃; 95~115 days later, plate count and most probable number showed that there was no culturable bacteria.

将大肠杆菌 O157：H7培养于低温贫养条件下，以涂布平板法和最大近似值法检测可培养细菌数，95～ 115d后表明可培养菌数下降为零。

Escherichia coli O157:H7 was cultured in sterilized fresh water at 4℃; 95~115 days later, plate count and most probable number showed that there was no culturable bacteria.

摘 要 将大肠杆菌O157：H7培养于低温贫养条件下，以涂布平板法和最大近似值法检测可培养细菌数，95~115d后表明可培养菌数下降为零。

Using microorganism fermentation cultivation technique, with escherichia coli strains of ASP - 311 for fermentation, with rich fumaric acid as the substrate to ferment produce l-aspartic acid, Study the result of activated carbon, and the temperature, the use of bleaching time and discoloring pH value of various conditions, aspartic acid decoloring effect, and finally: the concentration of carbon, decoloring temperature of 1.0% 60 degrees, the bleaching time for 0.5 h, pH for 6, in this condition, the result is the absorbency of decolour 3.2%, to achieve the best effect.

本文利用微生物发酵培养技术，以大肠杆菌ASP-311为发酵菌株，以富马酸为底物来发酵生产天冬氨酸；研究了发酵液中活性炭的使用量、脱色的温度、脱色的时间以及pH值等各种条件对L-天冬氨酸脱色效果的影响，最终得出：活性炭的浓度为1.0%，脱色温度为60℃，脱色时间为0.5h，pH为6，在此条件下，发酵液的吸光度为3.2%，此时的脱色效果达到最佳。

PDGF-BB is the most effective subtype in the PDGF family on the metabolism of osseous tissue or the treatment of wound healing, and its clinical application was approved firstly among the growth factors although it was used to treat the decubital ulcer of diabetes.

本研究通过分子克隆方法获取人血小板衍生生长因子B链成熟肽基因，构建原核表达载体；利用大肠杆菌体系原核表达人血小板衍生生长因子B链前体蛋白，并纯化、复性进而获得具有功能活性的PDGF-BB；并对获得的重组rhPDGF-BB蛋白进行体外功能活性鉴定，同时，初步探讨PDGF-BB在毕赤酵母中的表达，为寻找更优化的表达纯化方法提供实验学依据。

Objective Establishment of a hybridoma cell line secreting a monoclonal antibody to facilitate iˉdentification of human tissue kallikrein（KLK1gene,hK1protein）.Methods Mice were immunized with E.coli-exˉpressed GST-hK1fusion protein and their spleen cells were fused with SP2/0myeloblastoma cells.

目的 将人组织激肽释放酶（基因命名为KLK1，酶命名为hK1）cDNA克隆入大肠杆菌表达质粒，以重组菌表达的GST-hK1融合蛋白免疫小鼠，获得了分泌hK1特异单克隆抗体的杂交瘤细胞株。

This desaturase, when expressed in Escherichia coli, desaturated stearic acid to yield oleic acid, but did not desaturate other fatty acids in E. coli. These results indicate that PvFadA is specific to stearic acid.

通过将PvfadA基因在大肠杆菌表达系统中成功表达，PvFadA可以特异性地将C18：0脂肪酸转变成C18：1脂肪酸。

Results In animal study, lumbricus could inhibit the growth of staphylococci, bacillus coli and bacillus aeruginosus. The time of wound healing in experimental group was 4 days shorter than that in control group. At 4d and 7 day the numbers of the capillary, blood vessel endodermis and desmohemoblast desmocyte and splitting epithelium of trial group were much more than those of control group. At 4d the trial group′s numbers of splitting mesenchymal cell were much more than that of control group. From 3d on the wound healing and granulation filling of experimental group were much quicker than those of control group.

结果 动物实验中，地龙能抑制金黄色葡萄球菌、大肠杆菌及绿脓杆菌的生长；实验组创面愈合时间较对照组提前了4天；第4、7天实验组动物创面的毛细血管数、血管腔内皮细胞数及间质成纤维细胞数均较对照组明显增多，上皮细胞分裂象也高于对照组，第4天的间皮细胞分裂象也高于对照组；自第3天始创面愈合及肉芽充填速度实验组明显快于对照组。

Results: From the preliminary study, Desmodium gangeticum inhibited the biting and licking behavior induced by formalin in a dose-dependent manner. Additionally, it showed that Desmodium gangeticum could completely inhibit the growth of Pseudomonas and partly inhibited E. coli. in higher concentration. However, it had no effective on the Klebsiella species.

结果：预试实验中发现，大叶山蚂蝗粗抽物对於福马林所诱导小鼠产生的疼痛行为具有剂量依存性的抑制，在较高的浓度下，几乎可完全地抑制绿脓杆菌的生长及部分的大肠杆菌，但对克雷白氏菌则无影响。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。