大肠杆菌

This article microbiofilmtechonology fermention colibacillosis ASP-311 technology, to the strains to fumaric to slfia to fermetation asparate; qsdhmm oluv in movingbed emulsifications, decolourization decolourization of temperature, time, as well as the pH value of l - omniberaing conditionson decolouring asparate, the resulting effect of: movingbed or by 1.0% concentration, temperature is 60 700℃ decolourization, 0.5h, decolourization time for the pH of 6, in this condition, oluv of 3.2% absorbance at decolouring.

毕业论文翻译（本文生物学术语多，切勿使用在线翻译）本文利用微生物发酵培养技术，以大肠杆菌ASP-311为发酵菌株，以富马酸为底物来发酵生产天冬氨酸；研究了发酵液中活性炭的使用量、脱色的温度、脱色的时间以及pH值等各种条件对L-天冬氨酸脱色效果的影响，最终得出：活性炭的浓度为1.0%，脱色温度为60℃，脱色时间为0.5h，pH为6，在此条件下，发酵液的吸光度为3.2%，此时的脱色效果达到最佳。

The developed Indirect Sandwich ELISA was specific, the measure could not cross react to the negative antigen of duck swollen head septicemia virus, Duck Plague Virus, E. colibacillosis, Duck Hepatictis Virus.

建立的间接夹心ELISA法具有高度特异性，不与鸭肿头败血症阴性抗原、鸭大肠杆菌抗原、鸭瘟、鸭肝炎病毒抗原呈现交叉反应。

Firstly, MiCy, Citrine, mKO were inser into expression vectors of pRSETB, and then MiCy, Citrine,mKO were expressed in E.coli and purified by using Ni-NTA collum chromatography.

首先，构建了含有六个组氨酸标签的三种荧光蛋白MiCy、Citrine和mKO，而后在大肠杆菌中表达了MiCy、mKO和Citrine三种蛋白，并用金属鳌合亲和层析进一步纯化，纯化产物使用特异性酶去除His-tag。

Methods The coding gene of Sj32KD antigen was amplified from the cDNA library of the adult parasite by PCR and cloned into the prokaryotic expression vector pET28a to construct a re- combinant plasmid Sj32-pET28a.

应用PCR方法克隆Sj32KD抗原基因，并在大肠杆菌系统中进行表达，利用His亲和层析方法纯化融合蛋白，并应用重组的Sj32KD抗原检测羊日本血吸虫病。

Objective: To contructre combinant plasmid pET28a/hES and coexpress human Endostatin and predhPK-5 in E. coli. Methods: mRNA from human liver tissue was exracted, and the Endostatin gene was amplified by RT-PCR.

目的构建人内皮抑素原核表达质粒pET28a/hES，并与简化人纤溶酶原饼环区(predigested human PlasminogenKringle5，predhPK-5)在大肠杆菌中实现共表达。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

AFM images revealed that as the sputtering time was prolonged, the film thickness increased, the film became compacter, and the specific area of the film also increased, thus, the release rate of silver ions increased, which led to improved antibacterial properties. EDX test results indicated that increase in film thickness led to increase in silver weight percentage per unit surface, causing improved antibacterial properties. Moreover, when the film thickness was 1 nm, the inhibition percentage of Staphylococcus aureus and Escherichia coli were 100%.

结果表明：随着薄膜厚度的增大，样品抗菌性能提高；溅射时间延长，薄膜厚度增大，膜层的致密性改善，单位面积上的银含量增加，膜层表面积增大，银离子释放几率增大，是提高抗菌性能的主要原因；当纳米结构银薄膜厚度为1 nm时，对大肠杆菌和金黄色葡萄球菌的抑菌率均达到100%。

To elucidate whether Era is related to the death of bacterial cells expressed YggG294, A double promoter expression vector that can express YggG294 and Era proteins controllably in cells was constructed.

yggG是从大肠杆菌全基因组文库中钓取并克隆的Era结合蛋白基因，研究表明该基因可能表达产物YggG294蛋白对宿主菌的生长具有强烈的抑制作用。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。