大肠杆菌

Firstly,we isolated clinically and induced artificially multi-resistant Escherichia coli,detected antibacterials intake by different resistant level strains with CCCP and fluorescence spectro method and ascerained existing active efflux system in this strains; Secondly, construced internal standard of quantitive RT-PCR and detected AcrAB mRNA level of different resistant E.coli with quantitive RT-PCR; Thirdly, prepared AcrA antibody and detected AcrAB protein level of different resistant E.coli; eventually,designed and constructed Taqman probe of AcrAB and detected AcrAB level of different resistant E.coli with fluorescence quantitive PCR.

临床分离及人工诱导大肠杆菌多重耐药株，氰氯苯腙结合荧光分光法检测不同耐药水平菌株对抗菌药物的摄入，确证主动外排系统的存在；构建了定量RT-PCR的内标准DNA，通过定量RT-PCR检测动物源性大肠杆菌AcrAB mRNA的水平；制备AcrA抗体，采用Western-Blotting检测不同耐药株AcrAB蛋白的表达水平；设计和构建AcrA、AcrB的Taqman探针，检测不同耐药菌株AcrAB的水平。

In comparison with the reported corresponding sequences, the putative amino acid sequence shares high homology with assimilatory nitrate reductase of other microalgae, with identity 78% to Dunaliella tertiolecta, 60% to Volvox carteri, 58% to Chlamydomonas reinhardtii, and 50% to Chlorella vulgaris, respectively.

2.3 杜氏盐藻NR基因原核表达载体在大肠杆菌中的表达和功能鉴定(1)pMAL-NR-2组大肠杆菌SDS-PAGE结果显示：在140kDa左右出现一条特异性条带，分子量与MBP-NR融合蛋白相符（BMP+NR=42.5kDa+99.5kDa=142kDa），在IPTG（0.15mmol/L）37℃诱导时，该蛋白量占全菌蛋白的21.06％；对照组无此特异带。

The caprine IL-2 gene and the IFN-γgene without the signal peptidesequence were subcloned into (5") Bam H I and (3") HindⅢrestriction sites of pET30aplasmid, the recombinant plasmid were transformed into E. coli BL21(DE3) and induced withIPTG, they were demonstrated by SDS-PAGE that the genes were expressed as inclusion bodies.

应用DNA重组技术，将IL-2基因和去除信号肽的IFN-γ基因分别插入到原核表达载体pET-30a的BamHⅠ和HindⅢ位点上，构建原核表达质粒pET30a-CapIL-2和pET30a-CapIFN-γ，转化大肠杆菌BL21(DE3)，经IPTG诱导后进行SDS-PAGE电泳，结果表明目的基因在大肠杆菌中以包涵体形式融合表达。

In the paper,the control effect of these fators on colibacillosis was mainly discussed, and this provided theoretical basis for the control in the future.

主要探讨环境因素、疫苗免疫、药物预防等对猪大肠杆菌病的防控效果，为猪大肠杆菌病的防控提供理论依据。

By using the experiment animal and target animal to establish the pathology model of Colibacillosis, the experiment also measured out that matrine can effectively reduce the death rate of chicken caused by the Escherchia coli at the dose of 100mg/kg, showing that matrine has a good prohibiting effect on the Escherchia coli.

本实验还通过对实验和靶动物制造大肠杆菌病的病理模型，测定出苦参碱在100mg／kg的剂量下对大肠杆菌所致雏鸡死亡率有显著的降低作用。

With amikacin highly sensitive to the isolated strain of Escherichia coli as control, the cure and preventing protective test were made on colibacillosis in broilers infected artificially to evaluate its curative effect, Both Chinese herbal compound prescription and amikacin could control the clinical symptoms of broilers infected artificially by E.

方法］根据中兽医理论自拟中药复方制剂，以对分离株大肠杆菌敏感性较高的丁胺卡那霉素作为对照，对人工感染的肉仔鸡大肠杆菌病进行治疗和预防保护性试验，对其疗效进行评价。

Owing to growth rapidly and predominance in fecal contaminated water, we could detect and identify culturable E. coli cells in 50 mL of pond water in campus within one working day.

此外，本方法样品需求量低且不需繁复的前处理步骤，分析环境水样中之大肠杆菌，可在一个工作天内得到结果，为大肠杆菌检测提供一个快速、简单及经济的方法。

2Ai. In addition, cell extracts from E. coli JM109(DE3) cells harbouring the recombinant plasmid pAM28 were able to decarboxylate the histidine present in the reaction to histamine, whereas extracts prepared from control cells containing the vector plasmid alone did not.

此外，从大肠杆菌细胞提取物大肠杆菌JM109中（DE3）中细胞重组质粒pAM28窝藏能够decarboxylate在本组氨酸为组胺反应，从控制细胞制备含有载体质粒提取物，而仅仅没有。

The multi-copy expression vector pZM is a shuttle plasmid which can replicate in Escherichia coli, Streptomyces and S. erythraea. It contains PermE promoter, fd terminator, multiple clone site, thiostrepton and ampicillin resistance genes, and ColE1 ori and pJV1 ori replicons which make the vector replicate in E.

游离型多拷贝表达载体pZM是可以在大肠杆菌、链霉菌和糖多孢红霉菌中扩增的穿梭型质粒，带有PermE启动子、fd终止子、多克隆位点、硫链丝菌肽和氨苄青霉素抗性基因以及在大肠杆菌和糖多孢红霉菌中复制的ColE1 ori和pJV1 ori复制子，载体大小9880bp。

The effects of narciclasine isolated from Narcissus tazetta bulb on the growth of two model microorganisms, E.coli and Ceteuisiae fermentum, were studied in this paper. The results revealed that the growth of Ceteuisiae fermentum was obviously inhibited by 20μ.g/mL NCS. The inhibition was about 51%. However, the inhibition of NCS to E.coli growth was hardly observed.

本文以两种模式微生物—大肠杆菌和啤酒酵母为实验材料，对从水仙鳞茎中提取的生物碱Nareiclasine在两种模式微生物生长中的作用进行了比较研究，结果发现在20μg/mL NCS处理下，酵母细胞生长受到明显抑制，抑制率达到51%，而大肠杆菌生长则不受影响。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失