大肠杆菌

Abjective to express the Hepatitis A virus genome cDNA attenuated salmonella Typhimurium and to find the immunity to hepatitis A virus after administrated that salmonella Typhimurium with hepatitis A virus gemome to the mousehepatitis A virus coding region cDNA were inserted expressive plasmid PBV221 and then were transform into E coli DH5α.

使甲型肝炎病毒基因组cDNA能够在减毒伤寒杆菌中表达，从而通过口服此种减毒伤寒杆菌达到对甲肝免疫的作用。将甲肝病毒编码区cDNA插入高效表达质粒PBV221，转化大肠杆菌DH5α，筛选并鉴定阳性克隆。

Major secondary complications of methylmalonic acidemia include mental retardation, tubulointerstitial nephritis with progressive impairment of renal function, metabolic stroke and movement disorders, pancreatitis, growth failure, acrodermatitis-enteropathica-like lesions, and functional immune impairment.

甲基丙二酸血症主要的并发症包括智力障碍、肾小管间质性肾炎及肾功能渐行性衰退、代谢性中风和运动失调、胰腺炎、生长不足、大肠杆菌性肢皮炎样损伤和功能性免疫缺陷。

In this study,we have ascertained AcrAB mediated multi-resistance and set up molecular detection method in Escherichia coli isolated from livestock and avian.

本研究以畜禽大肠杆菌为研究对象，确证AcrAB介导的多重耐药性，并建立分子生物学检测方法。

And then the expressing level of AcrAB was detected by Western blotting. The results indicated that the level of resistance to fluoroquinolones of isolates appeared closely correlated with substitution in GyrA and ParC, but expressing level of AcrAB had not significant changes.

序列分析及AcrA的Western blotting检测结果表明，临床分离的鸡大肠杆菌对喹诺酮类药物的耐药水平与GyrA和ParC的突变密切相关，而AcrAB外输泵的表达水平无显著变化。

Some gene mutations of rob gene of the 5 E.coli isolates were related to animal species and to the level of drug resistance by affecting on the expression level of AcrAB efflux pump.

不同源性大肠杆菌的rob基因的核苷酸序列与其动物源性有关，该基因的核苷酸序列的个别突变位点可能影响AcrAB外输泵的表达水平，进而影响其多重耐药水平。

Keywords: Escherichia coli;AcrAB;efflux pump;resistance

中文关键词：大肠杆菌；AcrAB；外输泵；耐药性

Following purification by Ni affinity chromatography and stepwise renaturation by dialysis, rB7-B5 showed sound antimicrobial activity in porcine actinobacillus pleuropneumoniae and antibiotics-resistant E. coli. The present study provides basis for the research and development of novel antibacterial preparations.

以经Ni亲和层析柱纯化和多步透析法复性的rB7-B5，对猪胸膜肺炎放线杆菌和耐药性大肠杆菌具有很好的抑菌活性，本研究为新型抗菌制剂的研制和开发奠定了基础。

Compared with the Giant panda in captivity, the predominantintestinal florae of Giant panda returned to wild were still enterobacteria(Escherichia coli, Enterobacter aerogenes, Proteus, Salmonella, Yersinia enterocolitica), enterococcus and lactobacillus after Giant Panda had been returned to wild for a week.

与圈养大熊猫相比，放归大熊猫在放归一周后，肠道正常菌群中优势菌群仍为肠杆菌(大肠杆菌、产气肠杆菌、变形杆菌、沙门氏菌、小肠结肠炎耶尔森氏菌)，肠球菌和乳杆菌，但细菌数量即开始发生了一定变化，表现在肠球菌数量明显增加，乳杆菌数量明显减少，肠杆菌数量有轻微减少，到采样后期，肠球菌数量超过肠杆菌；另外，放归大熊猫粪样中芽抱杆菌和酵母菌的检出率较高，分别为60%和40%。

Conclution: Aerogenesis variety strains type 14 are commonly exist in shandong province, they have high relevancy with E. coli O32 phenotype traits such as biochemical nature and antigen structure, which indicates we should pay attention to differential diagnosis on these two strains.

C14型产气变种菌株在我省普遍存在。C14型产气变种与大肠杆菌O32的生化特性和抗原结构等表型特性具有高度的相关性，提示在检验工作中应注重对两种菌株的鉴别诊断。

Here we summarize the progress of salmon calcitonin's heterogenous expression in prokaryotic and eukaryotic expression systems as well as the amidation of C-terminal. Both the biological activity of salmon calcitonin and the biologic expression system can be improved by genetic engineering techniques.

本文综述了鲑鱼降钙素基因在大肠杆菌、链霉菌、乳酸杆菌和蓝藻等原核细胞，在酵母、昆虫、动植物等真核细胞中异源表达的研究进展，以及通过基因工程的方法能够改进生物表达系统，提高鲑鱼降钙素活性的动态。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。