大肠杆菌

The principal purpose of this study is to set up the isolation methods for diphtheria toxin expressed by genes, to purify the toxin with complete biological activities, and to make researches on its toxicities. In the experiments, the target gene was inserted into the cloning vectors--pET22b, then the vectors were introduced into host cells -BL21(DE3). The expression of the toxin was induced by addition of IPTG. The expression products were purified by His-6 label affinity chromatography. Then the diphtheria toxin expressed was transferred to PVC membrane using semidry blotter to determine the amino acid sequence of N-terminal. The "nicked" diphtheria toxin linked by disulfide bond was gained by protease hydrolysis.

为建立重组基因表达白喉毒素的分离纯化方法，制备出具有生物活性的蛋白质毒素，构建了白喉毒素表达载体pET22b-DT，并转入大肠杆菌BL21(DE3)中诱导表达，以融合表达的His-6作为标签通过亲和层析纯化；表达毒素经蛋白酶酶切后，形成了以二硫键连接的与天然结构相同的带"缺口"的白喉毒素，并测定白喉毒素对豚鼠的急性毒性和细胞毒性。

Sepiolite powder worked by hydrochloric acid was used as a sorption carrier of abio-antimicrobial, which was characterized by SEM and EDS analysis, and their sterilization capability to E. coli were investigated.

以酸改性后的多孔海泡石为载体制备了负载金属离子的无机抗菌别，利用SEM和EDS分析手段时所制备的无机抗菌剂进行了表征，并研究其对大肠杆菌的杀灭效果。

To construct the recombinant plasmid of protein 38kDa of M. tuberculosis and express, purificate and renaturate fusion protein 38kDa and to evaluate its potential value for TB serodiagnosis.

目的 构建结核分枝杆菌38kDa的重组质粒，在大肠杆菌中表达、纯化和复性重组蛋白，并评价其在结核病血清学诊断方面的价值。

In order to evaluate the potential of this immunodominant peptide antigen in serodiagnosis of TB, we have undertaken cloning and expression of a large number of esat-6p1 genes in Escherichia coli and purification products.

本实验研究目的是通过构建结核菌ESAT-6蛋白免疫优势段P1的重组多表达载体，在大肠杆菌 BL21（DE3）中进行诱导表达，并对表达产物进行纯化和复性，以获得大量ESAT-6P1抗原，同时对重组多免疫反应性进行鉴定评价，为探索重组多在结核病血清学诊断的应用奠定前期实验基础。

Methods: The traditional method of chronic serum sickness was modified by adding followings: one kidney removed, intraperitoneal inject LPS etc., and the effect of Shenhua Tablet on 24h proteinuria, blood biochemical indices, kidney function, pathology and immunofluorescence changes was observed compared with Fosinopril, and and the effect of Shenhua Tablet on the ED-1 positive cell number in the glomerulus, protein and mRNA expression and activation of Stat3 and protein and mRNA expression of TIMP-1 in the kidney tissue, and a-SMA positive mesangial cells were observed compared with Fosinopril using western blot, northern blot and immunohistochemistry methods.

对传统的大鼠慢性血清病肾炎模型制作方法进行以下改良：切除大鼠一侧肾脏、腹腔注射大肠杆菌内毒素、以及改尾静脉注射为腹腔注射。与西药福辛普利作对照，观察中药肾华对改良慢性血清病肾炎大鼠尿蛋白、血生化、肾功能、肾脏病理变化的影响。并通过免疫组化、Western蛋白印迹及Northern杂交方法，观察中药肾华对改良慢性血清病肾炎大鼠肾组织肾小球内炎细胞浸润、Stat3蛋白与基因表达及活化、以及其下游调控的TIMP-1蛋白与基因表达、系膜细胞增殖的影响。

In this article two groups of Shigella and E. coli strains that have identical O-antigen structure were studied, S.

同时本文还对另外一组有同样O-抗原结构的鲍氏志贺氏菌8和大肠杆菌O143的O-抗原基因簇进行了分析。

It Shigella, E. coli and Staphylococcus aureus and other bacteria have a strong inhibitory effect, a "natural antibiotic" the name.

它对痢疾杆菌、大肠杆菌和金黄色葡萄球菌等多种细菌都有较强抑制作用，有"天然抗生素"的美称。

Single- step method of RNA isolation by acid guanidinium thiocyanate－phenol－chloroform extraction.

重组人骨形成蛋白2成熟肽在大肠杆菌中的表达及其诱导成骨活性。

The chlorine dioxide disinfection solution was produced by the reaction between sodium chlorate and hydrochloric acid in chlorine dioxide generator.Filter membrane method was used to observe its efficacy in killing Escherichia coli in water and its influencing factors.

二氧化氯消毒液由二氧化氯发生器将氯酸钠与盐酸反应生成，采用滤膜法对其杀灭水中大肠杆菌的杀灭效果与影响因素进行了观察。

S extract had an inhibition effect against Sphaerotheca fuligenea,The inhibition rate was 100% when extract concentration was 20mg/mL and the inhibition rate got to 67.66% even at the concentration of 2.5mg/mL. The extract of E. humifusa had different preventive effects against four strains of S. fuliginea from four districts in Shanghai. The preventive effects on the Qibao and Baoshan were better than those of Nanhui and Qingpu. The microorganism inhibition experiment by filter papers indicated that the extract of E. humifusa had higher inhibition effect against bacteria than on pathogenic fungus. Diameters of the zone of inhibition of salmonellas, Echerichia coli and Staphylococcus aureus were 15.77mm, 12.27mm and 10.53mm respectively. The extract of E. humifusa had a certain effect against Colletotrichum lagenarium, Botrytis cinerea, Rhizoctomia solan and Pyricularia oryza but no effect against Helminthosporium maydis and Gibberella zea. The 4 cation exchange resins and ethanol deposition were used to isolate the active fractions from supernate after alkali deposition.

提取液对黄瓜白粉病菌体外分生孢子萌发抑制实验表明地锦提取液对黄瓜白粉病菌分生孢子的萌发有很好的抑制作用，抑制率随着地锦提取液浓度的增大而增高，当浓度为20mg/mL时，抑制率达到100%，即使在浓度为2.5mg/mL时，抑制率也达到了67.66%；室内盆栽苗期预防性实验表明了地锦提取液对上海4个不同地区的黄瓜白粉病菌均具有不同程度的抑制效果，对七宝和宝山地区的菌株抑制效果优于南汇和青浦地区的菌株；用滤纸片法测定了地锦提取液的抑菌谱，实验结果表明地锦提取液对供试的4种植物病原真菌：黄瓜炭疽病菌、黄瓜灰霉病菌、水稻纹枯病菌和水稻稻瘟病菌均具有一定的抑制效果，而对玉米小斑病菌和小麦赤霉病菌没有抑制效果，对供试的3种人类致病细菌沙门氏菌、大肠杆菌和金黄色葡萄球菌均具有很好抑菌效果，抑菌圈直径分别为：15.77mm， 12.27mm和10.53mm。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。