大肠杆菌

Recently, it was reported that RppHis a pyrophosphohydrolase, a member of the Nudix family, which removes pyrophosphate from the RNA 5'ends.

近来，有研究报道大肠杆菌Nudix水解酶家族的一员——RppH，可以从RNA的5'端除去焦磷酸根。

Coli lpdA mutant has the potential to produce pyruvic acid from xylose and mannose.

这些结果对构建以大肠杆菌为母体的模式"细胞工厂"有参考价值。

As a new recombination system, Red recombination has been widely used in E.coli gene knockout.

Red重组作为一种新的重组系统已经被广泛用于大肠杆菌的基因敲除、基因替换。

Yesterday, the Securities and Futures Commission in the implementation of the norms of the new valuation of the Fund for a work in the future, the funds have reduced the company's suspension related to the valuation of the shares of the Fund.

涉及减少超过该基金停牌的股票净值为基础的公共200.00亿元大肠杆菌…。。昨天，在对一个在今后的工作基金的新估价规范执行证券及期货事务监察委员会，资金减少了公司暂停相关基金的股票价值。

Methods Split the recombinant E. coli for expression of rhOP-1 by ultrasomication in ice bath and extract the inclusion body. Dissolve the inclusion body with 8mol/L urea, purify by SP-Sepharose chromatography, refold by gradient dialysis.

将表达rhOP-1的大肠杆菌菌体在冰浴下超声裂解，分离提取包涵体，用8mol/L尿素溶解，纯化后进行梯度透析复性。

Source: E. Reif, Munich Paleontological Society, Saarland University.

来源：大肠杆菌赖夫，慕尼黑古生物学会，萨尔河大学。

Results The relative molecular mass of expressed product was 34 000, which was consistent with that of GST-AEP fusion protein.

结果 大肠杆菌经诱导，高效表达出相对分子质量约34000的蛋白，与GST-AEP融合蛋白相符。

The die was cast from the approved design prepared by Lieutenant Aymar E. Embry, Engineers Officer Reserve Corps.

在木已成舟中将艾马由大肠杆菌安莉芳，工程师主任后备队编制的经批准的设计。

Citri had been selected by ribosome display in previous research. In order to express the scFvs with bio-activity efficiently, the genes of the selected scFvs were transformed into Escherichia coli HB2151 for expressing resoluble antibodies. The expression levels of scFvs were detected by dot blotting and Western blotting, and the expressed scFvs were purified by affinity chromatography, and then the activity of the purified scFvs was tested by enzyme-linked immunosorbent assay .

Citri， Xac高亲和力单链抗体(Single chain variable fragment， scFv)GX13、GX44和GX95，为高效表达具有生物活性的单链抗体，本研究将前期筛选的高亲和力单链抗体基因转入大肠杆菌HB2151中进行可溶性表达，点印迹和Western印迹检测可溶性单链抗体的表达水平，亲和层析纯化表达的单链抗体，酶联免疫吸附法检测纯化单链抗体的抗原结合活性。

We have demonstrated that the renatured Escherichia colioverexpressed RpoⅠ can reconstitute with Rhizobium core RNA polymerase in vitro. A preparation procedure has been established, which is expected to be useful to further functional study.

我们证明了大肠杆菌表达变复性的RpoⅠ能够与根瘤菌的核心RNA聚合酶在体外重组成全酶，并建立了成熟的制备方案，为以后阐明RpoⅠ的功能奠定了基础。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。