大肠杆菌

Results: The antibacterials experiment showed that the usnic acid material and the SMEDDS had inhibitory action to staphylococcus aureus and pneumococcus, but no inhibitory action to pseudomonas aeruginosa and bacillus coli.

结果 松萝酸原料药及其自微乳制剂对金黄色葡萄球菌和肺炎球菌的生长有抑制作用，对绿脓杆菌和大肠杆菌无效。

Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants.Pokeweed antiviral protein II is expressed with high level in summer leaves. The expression of PAPII is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPII gene by RT-PCR and then the gene was cloned into E.coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPII gene were then transferred into E.coli strain BL21 (DE3)-plysS and Pachia pastor is GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

Indirect enzyme-linked immunosorbent assay for detecting antibodies against avian influenza virus was developed by using expressed full length nucleoprotein of H9N2 AIV in E.coli.263 chicken serum samples(including 243 clinical serum samples and 20 positive serum samples from H9N2 AIV vaccinated chicks) were detected by NP-ELISA,agar gel precipitin test,and hemagglutination inhibition.

以大肠杆菌系统表达的H9N2亚型禽流感病毒核蛋白为抗原，建立了禽流感间接酶联免疫吸附试验抗体检测技术。

The gene was cloned into a prokaryotic expression vector and high efficiency expression was realized in E.coli.

结果 克隆了中国人肥胖基因，其cDNA顺序与已报道的白种人的序列完全一致，并成功在大肠杆菌中获得表达。

Objective To construct esat6-ppe68 fusion gene and its prokaryotic expression vector for expression in E.

目的 构建结核分支杆菌esat6-ppe68融合基因及其原核表达载体，在大肠杆菌中表达融合蛋白ESAT6-PPE68。

Pronucleus expression recombinant pMAL-C2X-HBV-X is constructed successfully and with the IPTG induction, the recombinant pMAL-C2X-HBV-X can express the X protein in E. coli JM109, which lays the foundation fur the HBV X protein purification and its biological study.

成功构建了HBV X基因原核表达重组体pMAL-C2X-HBV X；在IPTG的诱导下，该重组体在大肠杆菌中可表达X融合蛋白，为进一步纯化HBV X蛋白和研究其生物学作用奠定了基础。

In order to clone the VIP gene in the gastrointestinal tract from beijing duck, one pair of specific primers to VIP gene was designed and synthesized according to the chick sequence (X80906). Encoding VIP cDNA fragments were amplified by RT-PCR from the total RNA in the Proventriculus, the Duodenum and the Jejunum of Beijing duck. Their PCR products were ligated into pGEM-T easy vector, which was transformed into E. coil JM109. Positive bacteria clones were screened and identified by PCR method and digested with the double restriction enzyme EcoRⅠ. The sequence of VIP gene fragment was also determined and analyzed.

为从北京鸭胃肠道中扩增血管活性肠肽基因，根据鸡VIP基因（GenBank登录号X80906），设计了一对简并引物，从北京鸭腺胃、十二指肠和空肠提取总RNA，通过反转录－聚合酶链反应扩增，将从腺胃、十二指肠和空肠中扩增出的产物克隆到pGEM-Teasy载体上，导入大肠杆菌JM109，阳性克隆经双酶切鉴定后测序，将测序结果与鸡和鹅（GenBank登录号为DQ023161）的VIP基因进行同源性比较。

The purpose of this research is to generateengineering antibody by using phage display technology,and to investigate its binding activitywith the pseudorabies virus.

本研究的目的就是克隆抗伪狂犬病病毒抗体重链、轻链可变区基因，构建单链抗体基因，利用噬菌体展示技术制备噬菌体抗体，并在大肠杆菌中进行可溶性表达，获得具有对伪狂犬病病毒结合活性的表达产物，为以后在治疗、预防上的应用奠定基础。

This product can decrease ammonia and other putrefaction, inhibit amine produced by bacteria in intestine, neutralize E.

本品的使用可减少氨及其它腐败物质的生成，阻碍肠内细菌产生胺，中和大肠杆菌内毒素物质及外源物质的解毒，降低禽畜腹泻机率。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。