大肠杆菌

A multiplex PCR assay for detection of Escherichia coli O157:H7 was developed by using 3 sets of primers that specifically amplify segments of the rfbE、fliC and eaeA genes. The target genes fragment of the PCR assay were 291 bp, 625 bp and 368 bp, respectively. Analysis of 30 strains demonstrated that this PCR system was specific.

以编码大肠杆菌O157抗原的rfbE基因、编码H7抗原的fliC基因以及编码毒力因子的eaeA基因为靶基因，选择3对引物，建立并优化了检测大肠杆菌O157：H7的多重PCR体系，扩增产物分别为291 bp、625 bp、368 bp，采用30株细菌验证了该多重PCR具有特异性。

Department of Microbiology and Immunology is primarily dedicated to teaching and research in protein secretion in Escherichia coli, cell wall synthesis and cell division in Escherichia coli, interactions and functions of proteins, mechanism of DNA replication arrest, microbial pathogenesis, microbial genetics, immunodeficiency, immunogenetics, infectious diseases, molecular biology, etc.

微生物学与免疫学系主要致力于微生物学与免疫学的教学与研究，其研究领域包括大肠杆菌的蛋白质分泌，大肠杆菌细胞壁合成和细胞分裂，蛋白质的相互作用和功能，DNA 复制抑制机理，微生物致病机理，微生物遗传学，免疫缺陷，遗传学，传染病，分子生物学等。

Based on the results of water quality (physical and chemical factors: water temperature, salinity, pH, DO, DIN, PO4-P, COD, and biomarker-indicated: total bacteria and Coliforms) determining from the 4 pools and infall, outfall of the Xishan Island aquaculture pond, the variation characteristics of water quality in infall, outfall of pond and its reasons were analysised and discussed.

结果表明：养殖场和进、排口水体温度、盐度变化不明显，pH下降与养殖周期有关；营养盐含量增加，在不同养殖种类、养殖周期和养殖饲料系数变化不同；受消毒杀菌等措施影响下细菌总数与大肠杆菌总数明显减小，而养殖周期长的池塘，细菌总数仍增加，大肠杆菌总数减少幅度变小。

The results of batch equilibrium experiments show adsorption of E. coli on sand is accordance with linear isotherm.

批量平衡法测定大肠杆菌在砂土和溶液中的平衡分配系数的结果表明，大肠杆菌的吸附符合线性吸附。

The method does not need any special instruments and is more convenient than the methods that we have mentioned. It is an efficient means for rapid diagnosis. In addition, mutiple-clone rabbit IgG is made by the lipoid A of E. coli.

另外，多克隆兔抗大肠杆菌抗体IgG由大肠杆菌的类脂A制成，具有广谱免疫原性，肠杆菌科细菌的类脂A结构基本相同，可进行交叉免疫反应。

Oxford cup in vitro antibacterial medicine cup showed that it had vitro inhibitory action on beta streptococcus and colibacillus with crude drug content of 0.5g/ml; it had vitro inhibitory action on staphylococcus aureus,pneumococcus, beta streptococcus and colibacillus with crude drug content of 1.0g/ml,and decreased the death rate of mice caused by staphylococcus aureus.

牛津杯法体外抗菌试验表明0.5g生药量/ml时对乙型链球菌、大肠杆菌有体外抑制作用，1.0g生药量/ml时对金黄色葡萄球菌，肺炎球菌，乙型链球菌，大肠杆菌有体外抑制作用，降低金黄色葡萄球菌引起的小鼠病死率

And target protein can be secretively expressed in Pichia pastoris, this is helpful for separation and purification of target protein.Therefore this research intends to compare expression of Core protein in E. coli with in Pichia pastoris.

与大肠杆菌相比，毕赤酵母是单细胞真核生物，既具有生长快的特点，又能有效克服大肠杆菌系统缺乏蛋白翻译后加工、修饰的不足，表达外源蛋白可分泌到胞外，便于产品的分离纯化。

No squalene synthase polypeptide of expected molecular mass was observed in E. coli containing the putative full-length squalene synthase cDNA, however, overexpression in E. coli was achieved by truncating 30 hydrophobic amino acids at the carboxy terminus.

但在含有全长的鲨烯合酶cDNA的大肠杆菌中并没有观察到预期大小的鲨烯合酶表达，而C末端截短30个疏水氨基酸的鲨烯合酶可在大肠杆菌中过量表达。

The gene encoding leucyl-tRNA synthetase was cloned into this vector and expressed in high level.

将编码大肠杆菌亮氨酰-tRNA合成酶的基因插入到pMFT7H6的多克隆位点并在大肠杆菌JM109（DE3）中得到高表达。

Methods: The coding region of superoxide dismutase was amplified using PCR method from the E.co1i genome. The PCR product was cloned into PUC19-T vector and sequenced. In addition, the cloned coding region of Mn-SOD was inserted into the expression vector PET-28a to form the recombinant plasmid PET-28a-Mn-SOD and was then transformed into E.coli BL21 for expression.

用PCR方法从大肠杆菌2号基因组中扩增Mn-SOD基因编码区，克隆到pUCm-T Vector，测定核苷酸序列；再将基因编码区克隆到原核表达载体PET-28a，构建含Mn-SOD基因的重组表达质粒，转化到大肠杆菌BL21中进行IPTG诱导表达。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。